HRT和载体对一体化生物膜反应器脱氮除磷效果的影响

ISSN1000-0054CN11-2223/N()JTsinghuaUniv(Sci&Tech),20094932009,Vol.49,No.3w12　宁　涛2,3,　张丽丽1(1.,100084;2.-,518055;3.,518055):2008-04-10:(50238020,50478011);:(1971),(),,E-mail:[e-mail]:根据生物脱氮除磷原理,设计了一体化生物膜反应器,用于生活污水的处理。在一体化生物膜反应器处理生活污水连续运行基础上研究水力停留时间(HRT)和载体对反应器脱氮除磷效果的影响,同时通过分析微生物群落变化对系统脱氮过程进行探讨。试验结果表明:载体特性对反应器脱氮除磷效果影响显著;HRT为9h时,载体B4反应器总氮去除率为68.9%;好氧区HRT是限制系统脱氮效果的主要因素。由于载体的作用反应器好氧区存在着好氧缺氧微环境的交替,从而实现了好氧区同步硝化反硝化的脱氮过程。:水力停留时间;载体;脱氮除磷:X703:A:1000-0054(2009)03-0360-05EffectsofHRTandmediaonnitrogenandphosphorusremovalinanintegratedbiofilmreactorsGUANYuntao1,2,3,NINGTao2,3,ZHANGLili1(1.DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China;2.Tsinghua-KyotoCooperativeResearchandEducationCenterforEnvironmentalTechnology,Shenzhen518055,China;3.GraduateSchoolatShenzhen,TsinghuaUniversity,Shenzhen518055,China)Abstract:Anintegrativebiofilmreactorwasdesignedtotreatdomesticwastewaterforbiologicalnitrogenandphosphorusremoval.Theeffectsofthehydraulicretentiontime(HRT)andthemediaonthenitrogenandphosphorusremovalwerestudiedforcontinualtreatmentofdomesticwastewatercontinuouslyinanintegratedbiofilmreactor.Thenitrogenremovalisanalyzedbasedonthevarietyofthemicrobecommunity.Theresultsindicatethatmediacharacteristicssignificantlyimpactthenitrogenandphosphorusremoval.ForHRT=9h,thetotalnitrogenremovalratewas68.9%inthereactorusingB4media.TheHRTintheaerobiczoneistheprimaryparameterlimitingthenitrogenremoval.ThemediaaffectstheA/Omicroenvironment,producingsimultaneousnitrifying,anddenitrifyingintheaerobiczone.Keywords:hydraulicretentiontime(HRT);media;nitrogenandphosphorusremoval/(anoxic/oxic,A/O)A/O,,,,(simultaneousnitrifyinganddenitrifying,SND),[1-3],,,[4-6],,[5-6]A/O,,[6];,1　材料与方法1.11,,,8.6L,3.6L5L,7,5cm5cm5cm,CaMgSiFe3:A2cm2cm,;A42cm2cm,2;B44cm4cm,71,:(COD)50500mg/L,(SS)100400mg/L,350mg/L(CaCO3),pH6.57.5COD211,3,(AA4B4);2,1B4,1HRTh%1198931009013051002167229710023030091001.2COD;SS(VSS);;;-;-;pHpH-2;(BOD5)[7](denaturinggradientgelelectrophoresis,DGGE)(polymerasechainreaction,PCR)DNA,EUBf933GCEuBr1387PCRPCR450bp,8%60,100V40%60%,12h[6,8]2　结果与讨论2.121AA4B4COD(TN)(TP),COD111.2433.3mg/L,220.6mg/L,COD60mg/L,(GB18918-2002),B4A4A,A4A21,361,:HRT,AA4B4,A4B4A,,3,A,A4B4,AB4A4,B4,B4,,B4,,,B42,TN,TP(),;,,,,,,,31,[6],NO-2NO-3,,;,,B4,,,,,B4,312.2HRT1)HRTCODB4COD81%,DO,HRT3579hB4COD4COD111.2433.3mg/L,HRT,COD79.1%87.4%90.7%92.1%,COD50mg/L,A,HRT9hCOD24mg/L4B4COD2)HRT2B4HRT,HRTHRT362()2009,49(3),,2HRTHRT,,NO-2NO-3,85%,HRT,,HRT,HRTHRT3h9h,HRT,HRT9h,,HRT7h9h,HRT2HRTB4HRT/h/(mgL-1)/(mgL-1)/%335.042.729.837.014.913.3535.543.119.226.645.738.9743.456.413.121.169.161.6940.262.69.216.377.868.93)HRT5B4B45HRT,37.0%52.4%60.4%64.4%HRT7h9h,HRT,6.1mg/L9mg/L10.4mg/L11.2mg/L,7.2mg/L11.3mg/L13.0mg/L13.3mg/LHRT,,,HRTDO,-(PHB),2.36AA4B4DGGE,Labworks(UVP)DGGEH:H1=2.5547H2=2.1026H3=2.8869H4=3.6174H5=3.5975H6=3.7231H7=3.5846H8=3.2675H9=3.2215H,DGGE,B4AA4,,B46,1278911AA4B43,,89,6A4B489A,A4B4ASlot1,A;Slot2,A;Slot3,A;Slot4,B4;Slot5,B4;Slot6,B4;Slot7,A4;Slot8,A4;Slot9,A46B4AA4DGGE7B4363,:HRTDGGE,,,DGGE,(Band1-1,Band1-4,Band1-5,Band1-6,Band1-7,Band1-8,Band1-9),,NO-2NO-3(Band1-2)(Band1-10)(Band1-11),(Band2-3)(Band2-4)(Band2-7),,(Band2-9),,1,2;2,7;3,12;4,17;5,22;6,307B4DGGE3　结　论1),,A4B4AB42)HRTHRT3h9h,HRT,,B4HRT9h,COD3),(References)[1]AndradedoCantoCS.Feasibilityofnitrification/denitrificationinasequencingbatchbiofilmreactorwithliquidcirculationappliedtopost-treatment[J].BioresourceTechnology,2008,99(3):644-654.[2]ElisabethVM.Simultaneousnitrificationanddenitrificationinbench-scalesequencingbatchreactor[J].WatRes,1996,30(2):277-284.[3]RittmannBE,LangelandWE.Simultaneousdenitrificationwithnitrificationinsingle-channeloxidationditches[J].JournalWPCF,1985,57(4):300-308.[4]ChuangSH,OuyangCF,YuangHC,etal.EffectsofSRTandDOonnutrientremovalinacombinedAS-biofilmprocess[J].WaterSciTechnol,1997,36:19-27.[5]ZHANGXiuhong,ZHOUJiti.Nitrogenremovalperformanceinanovelcombinedbiofilmreactor[J].ProcessBiochemistry,2007,42:620-626[6],.[J].(),2007,47(6):822-825.ZHANGLili,GUANYuntao.Useofintegrativebiofilmreactorfordomesticwastewatertreatment[J].JTsinghuaUniv(Sci&Tech),2007,47(6):822-825.(inChinese)[7].[M].3.:,1989.[8]MuyzerG,DeWaalEC,UitterlindenAG.Profilingofcomplexmicrobialpopulationsbydenaturinggradientgelelectrophoresisanalysisofpolymerasechainreaction-amplifiedgenescodingfor16SrRNA[J].ApplEnvironMicrobiol,1993,59:695-700.[9].[D].:,2006.ZHANGLili.Studyondomesticwastewatertreatmentwithintegratedbiofilmreactoranditsmicrobiologicalcommunity[D].Beijing:TsinghuaUniversity,2006.(inChinese)364()2009,49(3)