INTETS活性及AUR和SOUR表征污泥活性的比较

29520165ＲesearchofEnvironmentalSciencesVol．29No．5May2016．INT-ETSAUＲSOUＲJ．2016295767-773．ＲONGHongweiZHANGYaokunZHANGChaoshengetal．ComparisonofINT-ETSAUＲandSOUＲforcharacterizationofsludgeactivityJ．ＲesearchofEnvironmentalSciences20162957[e-mail]-ETSAUＲSOUＲ1112331．5100062．2660003．510627Cd2+、Zn2+Cr6+NH4+-NCODCrINT-ETS、AUＲSOUＲ3．NH4+-NNH4+-NCODCr．NH4+-NAUＲ．Cd2+INT-ETSEC5019.164mgLCd2+Zn2+Cr6+AUＲEC5040.69127.117mgLZn2+Cr6+．3EC50Cd2+＞Cr6+＞Zn6+．X7031001-6929201605-0767-07ADOI10.13198j．issn．1001-6929．2016．05．20ComparisonofINT-ETSAUＲandSOUＲforCharacterizationofSludgeActivityＲONGHongwei1ZHANGYaokun1ZHANGChaosheng1WANGLianjie2WUZhijian3HUANGXiaopeng31．SchoolofCivilEngineeringGuangzhouUniversityGuangzhou510006China2．QingdaoTourismPlanning＆ArchitectureDesignInstitutionQingdao266000China3．GuangzhouSewagePurificationCo．Ltd．Guangzhou510627ChinaAbstractInordertoexploreamethodforrapidlyestimatingtheinhibitoryeffectofheavymetalsontheactivityofsludgetheinfluenceofCd2+Zn2+andCr6+onNH4+-NandCODCrremovalwasinvestigatedviabatchtestsofinjectingdifferentvolumesofheavymetalsolution．ThreeindexesincludingINT-electrontransportsystemactivityINT-ETSAmmoniaUptakeＲateAUＲandSpecificOxygenUptakeＲateSOUＲwereusedtocharacterizetheinhibitorysituationofheavymetalsontheactivityofsludge．TheexperimentalresultsshowedthattheinhibitoryeffectofheavymetalsonremovingNH4+-Nwasmoresignificantthandecomposingorganicsandnitrifyingbacteriaweremoresensitivethanheterotrophicbacteriatothetoxicityofheavymetals．Thisreflectsthetoxicinfluenceontheactivityofsludge．BesidescomparedwiththeinhibitionrateofNH4+-NremovalAUＲwasthemosteffectiveindexthatwasusedtocharacterizethetoxicityofheavymetals．ForCd2+INT-ETSactivitywasthemostsensitiveandoptimalparametersinceitsEC50valuewastheminimum19.164mgL．ForZn2+andCr6+AUＲactivitywasthemostsensitiveoptimalparameterbecauseitsEC50valuesweretheminimum40.691and27.117mgLrespectively．AndaccordingtoEC50valuesheavymetalswererankedinthesequenceofdecreasingtoxicityCd2+＞Cr6+＞Zn6+．KeywordsheavymetalactivatedsludgeINT-electrontransportsystemactivityammoniumuptakeratespecificoxygenuptakerate、1-3．294-5．6-8．、、9-1314．．ETS、SOUＲAUＲ15-17．INT+90mVETSSOUＲAUＲ．3———Cd2+、Zn2+Cr6+18-213、．11.1、、SBＲ．GB18918—2002《》A．、NH4Cl、NaHCO3、KH2PO3CaCl2、MgSO4、FeSO4．ρCODCr280～300mgLρNH4+-N25～30mgLρNO3－-N0.10～2.00mgLρNO2－-N0～1.10mgLρTP6.50～7.50mgLpH7.0～7.5.1.2ρCODCrρNH4+-NρNO3－-NρNO2－-NN-1--ρMLSSρDOpHWTW．INT-ETS2AUＲSOUＲ13．CdCl2·2.5H2O、ZnCl2、K2Cr2O7．1.31.3.1SBＲ1.0LρMLSS2000mgL．36．ⅠCd2+ρCd2+0、10、20、25、30、35mgLⅡZn2+ρZn2+0、15、30、45、60、75mgLⅢCr6+ρCr6+0、10、25、40、55、70mgL．．ρDO3～4mgLpH7.0～7.5.15minρNH4+-N5h15minρCODCrρNH4+-N．0.6mLINT-ETS．6250mL．ρDODOρDO．1.3.2INT-ETS、AUＲSOUＲ．=X0－XX0×100%X00mgLINT-ETS、AUＲSOUＲXINT-ETS、AUＲSOUＲ．ΓΓ=100%－Origin9.0ΓΓ=150%EC50．8675INT-ETSAUＲSOUＲ22.1Cd2+、Zn2+Cr6+NH4+-NCODCr1.1ρCd2+NH4+-N．ρCd2+35mgLNH4+-N10%．CODCrρCd2+25mgL．ρZn2+NH4+-NCODCrCODCrZn2+NH4+-NρZn2+45mgLNH4+-N50%．Cd2+Zn2+ρCr2+40mgLNH4+-N50%ρCr2+55mgLCODCr90%．Cr6+NH4+-N．1NH4+-NCODCrFig.1EffectofheavymetalsonNH4+-NandCODCrremovalrateNH4+-NCODCr22-2627．Cd2+DNA、28-29．Zn2+ρZn2+30．Cr6+Cr3+31．2.2INT-ETS、AUＲSOUＲ．2.1NH4+-NCODCr．INT-ETS、AUＲSOUＲNH4+-N．2～4Cd2+、Zn2+Cr6+INT-ETS、AUＲSOUＲNH4+-N．2Cd2+INT-ETS、AUＲSOUＲNH4+-NＲ20.65013、0.781360.726980.6AUＲNH4+-N．Cd2+AUＲNH4+-N．3Zn2+INT-ETS、AUＲSOUＲNH4+-N0.69973、0.881120.828640.6AUＲNH4+-N．Zn2+AUＲNH4+-N．4Cr6+INT-ETS、AUＲSOUＲNH4+-N0.60604、0.745190.730600.6AUＲNH4+-N．Cr6+AUＲNH4+-N．967292Cd2+NH4+-NFig.2TherelationshipbetweenNH4+-NremovalrateandtheactivityofsludgeundertheactionofCd2+3Zn2+NH4+-NFig.3TherelationshipbetweenNH4+-NremovalrateandtheactivityofsludgeundertheactionofZn2+4Cr6+NH4+-NFig.4TherelationshipbetweenNH4+-NremovalrateandtheactivityofsludgeundertheactionofCr6+Cd2+、Zn2+Cr6+INT-ETS、AUＲSOUＲ3AUＲNH4+-NAUＲSOUＲINT-ETS．2.3、INT-ETS、SOUＲAUＲ．EC50．EC50．EC50-“S”．50%“S”EC50．Origin9.0Γ5．95%Γ=150%EC50．Γ＞0.94.EC50EC501.Cd2+3INT-ETS＞AUＲ＞0775INT-ETSAUＲSOUＲSOUＲINT-ETSCd2+Zn2+AUＲ＞SOUＲ＞INT-ETSAUＲZn2+Cr6+AUＲ＞INT-ETS＞SOUＲAUＲCr6+．3EC50Cd2+＞Cr6+＞Zn6+．5ρCd2+、ρZn2+、ρCr6+ΓFig.5ＲelationshipcurveofρCd2+ρZn2+ρCr6+andΓ1ρCd2+、ρZn2+、ρCr6+ΓEC50Table1FittingequationofρCd2+ρZn2+ρCr6+andΓandEC50ρCd2+ρZn2+ρCr6+EC50mgLEC50mgLEC50mgLINT-ETSy=0.0034x2－0.0141x+0.0238Ｒ2=0.995019.164y=3.2406×10－4x2－0.0083x+0.0226Ｒ2=0.980269.219y=3.1418×10－4x2+0.0260x－0.0539Ｒ2=0.995929.780AUＲy=0.0062x2－0.1167x+0.1938Ｒ2=0.942524.174y=0.0020x2－0.0622x+0.2086Ｒ2=0.987040.691y=0.0012x2－5.7703×10－4x+0.1296Ｒ2=0.990427.117SOUＲy=0.0037x2－0.0687x+0.1144Ｒ2=0.945727.537y=7.0771×10－4x2－0.0158x+0.0366Ｒ2=0.987649.775y=9.7950×10－4x2－0.0169x+0.1349Ｒ2=0.978139.5893aNH4+-NCODCr．bNH4+-NINT-ETS、SOUＲAUＲCd2+、Zn2+Cr6+AUＲNH4+-N0.78136、0.881120.74519.cCd2+INT-ETSEC5019.164mgLCd2+Zn2+Cr6+AUＲEC5040.69127.117mgLZn2+Cr6+．3EC50Cd2+＞Cr6+＞Zn6+．Ｒeferences1HAMMAINIABALLESTEＲABLAZQUEZMLetal．EffectofthepresenceofleadonthebiosorptionofcoppercadmiumandzincbyactivatedsludgeJ．Hydrometallurgy200267123109-116．2．D．20102-21．3．J．200532436-39．LIUYoucaiZHONGHongLIUHongping．Ｒesearchpresen