pH对UASB运行效能及产甲烷互营菌群的影响张立国

45820138JOURNALOFHARBININSTITUTEOFTECHNOLOGYVol.45No.8Aug．2013pHUASB张立国，李建政，班巧英，许一平150090pH．UASBpH．COD20000mg·L－1、HRT8hpH6.95.4UASBpH6.8～7.45.7～6.7COD23.3%3.9．－PCR－DGGEUASBpH．Eub19PelotomaculumpHpHMethanobacteriumferruginisUnculturedMethanobrevibacter．UASBpH．UASBpHX703A0367－6234201308－0044－06ImpactofpHonperformanceandsyntrophiccommunityofhydrogen-producingacetogensandmethanogensinanUASBZHANGLiguoLIJianzhengBANQiaoyingXUYipingStateKeyLaboratoryofUrbanWaterResourceandEnvironmentHarbinInstituteofTechnology150090HarbinChinaAbstractAnmesophilicupflowanaerobicsludgebedUASBreactortreatingsugarrefinerywastewaterwithaninfluentCOD20000mg·L－1andHRT8hwasintroducedandtheimpactofpHonitsperformanceandhydrogen-producingacetogensandmethanogenswereinvestigated．TheresultsshowedthatthepHintheUASBdroppedfrom6.8－7.4to5.7－6.7whentheinfluentpHdecreasedfrom6.9to5.4resultinginareductioninCODremovalby23.3%andapropionateincreaseineffluentby3.9times．Polymerasechainreaction-denaturinggradientgelelectrophoresisPCR－DGGEofsampledsludgeindicatedthatthediversityofhydrogen-producingacetogensintheUASBwasremarkablylowerthanthatofthemethanogens．Thedistributionanddominanceofhydrogen-producingacetogenshadbeenobservablyinfluencedbythedroppedpH．Thedominanceofpropionate-oxidizingbacteriaPelotomaculumrepresentedbybandEub19weresignificantlyreducedinapproachacidconditionwhileacetotrophicmethanogensandthedominanthydrogenotrophicmethanogensstoodsteadily．Furthermorethedominanceofsomeacid-toleranthydrogenotrophicmethanogenssuchasMethanobacteriumferruginisandunculturedMethanobrevibacterwereenhancedobservablyfollowingthedropinpH．Theseresultssuggestedthathydrogen-producingacetogensseemedtobemoresusceptibletopHthanmethanogens．KeywordsupflowanaerobicsludgebedUASBpHperformancehydrogen-producingacetogensmethanogens2012－07－19．511781362010DX06．1980—1965—[e-mail]．、、1－2．3、3．4．5－67－89－10．pHpH．pH11－13．pH、．UASBpH－PCR－DGGEpH．11．11UASB1350mm11L3∶1．1000mm10∶1350mm．200mm51～5．35±1℃．．1.2KH2PO4ρCOD∶ρN∶ρP200∶5∶1pH．UASBCOD20000mg·L－1、ALK4500mg·L－1、pH6.9HRT8hCOD90%MLVSS37.54g·L－1．污泥床反应区悬浮区温控系统出水水封气表沼气1号2号3号4号5号取样口三相分离器沉淀区进水1UASB1.3pHUASBHRT8h、COD20000mg·L－1pH6.910dⅠ．CODHRTpH6.2Ⅱ11～44d5.4Ⅲ45～71dpH．1.4COD、pH、MLVSSALKCaCO314LML－1VFAsSP－6800ASP689015．1.5DNAPCR－DGGEDNADNAMOBioLaboratoriesInc．CarlsbadCAUSAPCRDGGEWan16．341F5'－CCTACGGGAGGCAGCAG－3'GC907R5'－CCGTCAATTCMTTTGAGTTT－3'．344F5'－ACGGGGYGCAGCAGGCGCGA－3'GC915R5'－GTGCTCCCCCGCCAATTCCT－3'．NCBIBlastXMEGA3.1．22.1pH2UASBpHpH．pH6.9Ⅰ·54·8pHUASB、6.2Ⅱ5.4ⅢUASBpH7.7、7.47.0．pHUASBpH．ⅠUASB1、35pH6.8、7.17.4pH6.2Ⅱ、pH6.3、6.87.1ⅢpH5.4、pH5.7、6.56.7．pHUASB、17－18．1号5号进水3号出水%ⅠⅡⅢ8.07.06.05.0010203040506070t/dpH2UASBpH2.2VFAsUASBVFAs8．ⅠUASBpH6.8～7.42COD20000mg·L－1HRT8hVFAs500mg·L－13．乙酸丁酸总挥发酸丙酸戌酸321010203040506070t/d籽(挥发酸)/(g·L-1)ⅠⅡⅢ3pHpH6.2ⅡUASBVFAs1368mg·L－163.1%22.8%3．pHUASBpHpH6.3～7.1219VFAsVFAs．332UASB．VFAsⅠⅠ21.9%pH．pH5.4ⅢpH5.7～6.7UASBVFAsⅡ．VFAs11241407mg·L－1．100mg·L－11200～1300mg·L－1．3UASBⅢ10d558．2.317．ⅠUASB440L·d－147%4．pHⅡ6.2218dUASB413L·d－1Ⅰ7%4．pHⅢ5.424Ⅰ24%6%．4－5UASB．Ⅲ100mg·L－1·64·451200～1300mg·L－13．．102030405060705004003002001000706050403020100甲烷体积分数产气量渍(甲烷)/%产气量/(L·d-1)t/dⅠⅡⅢ4pHCH42.4CODCOD12．UASBⅠCOD91.3%5．pH6.96.25.4UASBCODpH．3ⅡⅢ3pHCOD．COD5．UASBpH6.2ⅡCOD89.2%．pH5.4ⅢCOD68%8．252015105010080604020010203040506070t/d进水COD出水CODCOD去除率COD/(g·L-1)COD去除率/%ⅠⅡⅢ5pHCODCOD2.5pHUASBUASB3UASB1、35916SrDNAPCR－DGGE．6aDGGE、NCBIBlastXUASB1Eub1916SrDNAPelotomaculumschinkiistrainHHT96%6b1、20．19DGGEUASBpHEub19．ⅠEub19pH6.8pH7.1ⅡpH6.8pH7.1ⅢpH6.5Ⅱ．Eub19UASB、COD．1号3号5号Eub190.0ⅠⅡⅢⅠⅡⅢⅠⅡⅢsp.Eub19abIIIIII3pH7．06．35．51353．6·74·8pHUASBUASB．916SrDNAPCR-DGGE7．7b4Arc3、4、7、8Methanosaetaconcilii99%4Arc1、2、5、6、9Methanolineatarda、Methanobacteriumbeijingense、Methanobacteriumferruginis、UnculturedMethanobrevibacterMethanospirillumhungatei＞98%5H24．7a4Arc3、4、7、82Arc1Arc2pHUASB．pH．ⅠⅡArc9、ⅢArc5、6．M．ferruginisArc5pH5.5～8.021．VFAs4．COD20000mg·L－1、HRT8h、MLVSS37.54g·L－1pH6.96.25.4UASBVFAs1.63.93．Ⅲ7．pHpH．0.0Arc1Arc2Arc3Arc4Arc7Arc8Arc9Arc5Arc61号3号5号ⅠⅡⅢⅠⅡⅢⅠⅡⅢMethanobrevibacterArc5Arc2Arc9Arc8Arc3Arc7Arc4Arc6Arc1ab731COD20000mg·L－1、HRT8h、37.54g·L－1pH6.95.4UASBpH6.8～7.45.7～6.7COD23.3%3.9．2pHUASBpH．3UASBpH．1LETTINGAGFIELDJLIERJVetal．Advancedanaerobicwaste-watertreatmentinthenearfutureJ．WaterScienceandTechnology199735105－12．2ANGENENTLTKARIMKAL-DAHHANMHetal．Productionofbioenergyandbiochemicalsfrom·84·45industrialandagriculturalwastewaterJ．TRENDSinBiotechnology2004229477－485．3BRIONESARASKINL．DiversityanddynamicsofmicrobialcommunitiesinengineeredenvironmentsandtheirimplicationsforprocessstabilityJ．CurrentOpinioninBiotechnology2003143270－276．4LIUYWHITMANWB．MetabolicphylogeneticandecologicaldiversityofthemethanogenicarchaeaJ．AnnualNewYorkAcademyofScience20081125171－189．5MCINERNEYMJSTRUCHTEMEYERCGSIEBERJ．PhysiologyecologyphylogenyandgenomicsofmicroorganismscapableofsyntrophicmetabolismJ．AnnualNewYorkAcademyofScience2008112558－72．6．FISHJ．20074761038－1043．7SHIGEMATSUTERASMIZUNOYetal．Microbialcommunityofamesophilicpropionate-degradingmethanogenicconsortiuminchemostatcultivationanalyzedbasedon16SrRNAandaceta