PlackettBurman设计和响应面法优化试验

292009,Vol.30,No.062009,Vol.30,No.0630Fig.1StandardcurveofrutinFig.2EffectsofethanolconcentrationonextractionrateoftotalflavonoidsFig.3Effectsofultrasonicextractiontimeonextractionrateoftotalflavonoids312009,Vol.30,No.06Fig.4EffectsofratioofethanolsolutiontorawmaterialonextractionrateoftotalflavonoidsFig.5EffectsofinfiltrationtimeonextractionrateoftotalflavonoidsFig.6EffectsofextractiontimesonextractionrateoftotalflavonoidsTable1Plackett-BurmantestdesignandresultsTable2Partialregressioncoefficientsandtheirsignificancetest2009,Vol.30,No.0632Table3Box-BehnkendesignandresultsTable4AnalysisofvarianceofregressionequationFig.7ResponsesurfaceplotforeffectsofethanolconcentrationandratioofethanolsolutiontorawmaterialonextractionrateoftotalflavonoidsFig.8ResponsesurfaceplotforeffectsofethanolconcentrationandextractiontimesonextractionrateoftotalflavonoidsFig.9Responsesurfaceplotforeffectsofratioofethanolsolutiontorawmaterialandextractiontimesonextractionrateoftotalflavonoids332009,Vol.30,No.06H2O2B1,2,1,23,3,1,21.,4000452.,4000453.,400044:B,,pHH2O2,B,B,,.,pHH2O2.B:(B)100mgPL,pH4143216W,H2O21177mL.,B93134%.,,3148%.:;;;:X703:A:1001-6929(2009)03-0270-07OptimizationofDegradationofAzoDyeAcidGreenBbyLow2FrequencyUltrasoundSynergizedwithH2O2UsingResponseSurfaceMethodologyZHANGZhan2mei1,2,ZHENGHuai2li1,2,HUPeng3,JIFang2ying1,21.KeyLaboratoryofThreeGorgesReservoirRegionpsEco2Environment,StateMinistryofEducation,ChongqingUniversity,Chongqing400045,China2.FacultyofUrbanConstruction&EnvironmentalEngineering,ChongqingUniversity,Chongqing400045,China3.CollegeofChemistryandChemicalEngineering,ChongqingUniversity,Chongqing400044,ChinaAbstract:Onthebasisofasinglefactorexperiment,initialpHofAcidGreenBdyewastewater,dosageofH2O2andultrasoundpowerwereselectedasindependentvariables,anddegradationratioofAcidGreenBastheresponsevalue.ResponsesurfacemethodologywasemployedtoinvestigatetheeffectsoftheindependentvariablesandtheirinteractionsonultrasonicdegradationofAcidGreenB.Apredictivepolynomialquadraticequationmodelwasdevelopedbysolvingthequadraticequationaswellasanalyzingtheresponsesurfaceplotsandtheircorrespondingcontourplots.ANOVAandresponsesurfaceanalysisresultsshowedthatinitialpHofdyewastewater,dosageofH2O2andultrasoundpowerhadsignificantcorrelationwiththedegradationofAcidGreenB.TheoptimumconditionsofultrasonicdegradationofAcidGreenBwastewaterweredeterminedtobeinitialdyeconcentration100mgPL,pHofdyewastewater4143,ultrasoundpower216WandthedosageofH2O21177mL.93134%ofAcidGreenBwasdegradedundertheoptimalconditions.Theadequacyofthemodelequationforpredictingtheoptimumresponsevalueswasverifiedeffectivelybytheexperiment,andtheexperimentalvaluesagreedwiththepredictedvaluesofthemodelequationwith3148%deviation.Keywords:responsesurfacemethodology;ultrasound;degradation;azodye:2008-07-23:2008-08-29:(708071);(20777095);(200701Y1A0250211):(1980-),,,[e-mail].3,(1957-),,,,,,[e-mail],,,,,.,22320093ResearchofEnvironmentalSciencesVol.22,No.3Mar.,2009©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[123],,,.Box-Behnken,,,,.,[4].B,H2O2B,,Box-Behnken,,B.11.1KQ-250VDB();DELTA320(-);TU-1810-().30%()H2O2;011molPLNaOH011molPLH2SO4pH;(99199%),.1.2B.B21,2,,.-,200900nmB636nm.1.3200mLB,H2SO4NaOHpH,H2O2,100mLPmin,150min,,25,(Na2SO3PNa3PO4PKI)H2O2,636nmB.22.12.1.1(B)B200mL,pH4151180mLH2O2,,200W,(B)B,1.1(B)BFig.1EffectofinitialmassconcentrationondegradationofAcidGreenB1,(B)100300mgPL,B;(B)300mgPL,B.:(B),(B),[5];,,[627],OHOOH,,,.2.1.2pHB200mL(B)100mgPL1180mLH2O2,,200W,pH150min,pHB,2.2,B.:,pH,B,OHOOH[8],;pH,pHH+OH,B.pH,OHpH,B1723:H2O2B©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(B)100mgPL,pH415,1180mLH2O2,,B,3.3BFig.3EffectofultrasonicpoweronAcidGreenB3,B,.,,,,[9-10];,,,,B[11].,150250W.2.1.4H2O2B,,H2O2B,4.4,,H2O2B.:H2O2,;,H2O2OH[12],4H2O2BFig.4EffectofH2O2dosageondegradationofAcidGreenBH2O2,[13].H2O2,H2O2OH,OH[14],B.,200mL112210mLH2O2.2.22.2.1,Box-Behnken200mL,,x1,x2x3,H2O2pH,-1,0,+1.Xi=(xi-x0)Px,,Xi;xi;x0;x.1.1Box-BehnkenTable1LevelsandcodesofBox2Behnkendesign-10+1PWx1X1150200250H2O2PmLx2X21.21.62.0pHx3X33.04.05.0:X1=(x1-200)P50;X2=(x2-116)P014;X3=(x3-410)P110.,H2O2pH,B,B:=B0+B1X1+B2X2+B3X3+B12X12+B13X13+B23X23+B11X12+B22X22+B33X32,B;B0;B1,B2B3;B12,B13B23;B11,B22B33.27222©1994-2009ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:=90190+2147X1+3161X2+22134X3+1168X1X2+1108X1X3+3100X2X3-5165X12-6142X22-28113X323.4.2Box-BenhnkenTable2Box2BenhnkenexperimentplanandresultsX1X2X3x1PWx2PmLx3()P%1-1-101501.24.075.221-102501.24.076.03-1101502.04.078.341102502.04.085.85-10-11501.63.033.2610-12501.64.036.87-1011501.65.075.381012501.65.083.290-1-12001.23.032.8100112002.03.034.8110-112001.25.071.9120112002.05.085.9130012001.64.090.1140002001.64.091.1150002001.64.091.53Table3VarianceanalysisofregressionequationFProbF97222.89802.541033.54