SBR中生物除磷颗粒污泥的反硝化聚磷研究刘小英

SBR1,2,1＊,1(1.,　710055;2.,　710054):(DNPAOs)-3-(PHB),.SBR,(AAO),(PAOs).P90%,NOx-N93%,25～33mgL,1gNOx-NP1.3g;,(SRPR)18.39mg(g·h),(SUPR)23.72mg(g·h),(SDNR)18.19mg(g·h),SUPR17.15mg(g·h);DNPAOs14.9%80.7%.,0.16～0.7,0.0031.:;AAO;;:X703.1　:A　:0250-3301(2008)08-2254-06:2007-06-22;:2007-08-10:(50478047):(1975～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]ptakeofBiologicalPhosphorousRemovalGranularSludgeinSBRLIUXiao-ying1,ZHAOHong-mei2,PENGDang-cong1,SUIXian-jie1(1.SchoolofEnvironmental&MunicipalEngineering,Xi'anUniversityofArchitecture&Technology,Xi'an710055,China;2.DepartmentofEnvironmentalScienceandEngineering,Chang'anUniversity,Xi'an710054,China)Abstract:ThedenitrificationandexcessivePremovalcanberealizedbydenitrifyingphosphate-accumulatingorganisms(DNPAOs)underlowcarboncondition.DNPAOsusepoly-3-hydroxy-butyrate(PHB)aselectrondonor,andusenitrateandnitriteaselectronacceptorduringthedenitrifyingphosphateuptake.Inthisstudythebiologicalphosphorusremovalgranularsludgewasinducedintothedenitrifyingandphosphateuptakegranularsludgeunderananaerobicanoxicaerobicalternatingoperation(referredtoasanAAO)inasequencingbatchreactor(SBR).Whenthesystemisstable,thePandNremovalratioisover90%and93%respectively;thereleasephosphorusis25-33mgLatanaerobicstage;1gNOx-NapproximatelytakesupP1.3gatanoxicstage.Inthetypicalcycletheanaerobicmaximumspecificreleasephosphorusrate(max.SRPR)is18.39mg(g·h);theanoxicmax.specificuptakephosphorusrate(SUPR)andthemax.specificdenitrificationrate(SDNR)is23.72mg(g·h)and18.19mg(g·h)respectively;theaerobicmax.SUPRis17.15mg(g·h).ThefractionofDNPAOsrisesfrom14.9%to80.7%.Comparedwiththephysical-chemicalpropertyofthebiologicalphosphorusremovalgranularsludge,thesettlingvelocityandthespecificgravityofthedenitrifyingphosphateuptakegranularsludgeiselevated0.16-0.7timesand0.0031respectively.Keywords:sequencingbatchreactor(SBR);AAO;granularsludge;denitrifyingphosphateuptake　　(SBR),.,SBR、、[1～7].、、.DNPAOs[8～10].DNPAOsPAOs[11,12],(PHAs),(),(),,[13,14].、.,,.,SBR(AAO),AAO,.1　1.1　SBR,31.529820088　　　　　　ENVIRONMENTALSCIENCEVol.29,No.8Aug.,2008DOI:10.13227/j.hjkx.2008.08.026cm,16cm,5L,4L,,(1).4h,、、、5,、、3.2L(12)1min,COD25mgL,70～105min.1～5min7～35mL,5mgL10mgL.,,NOx-N1mgL.,,300rmin;0.08～0.15m3h,20～37.5m3(m3·h);200～300mL,(SRT)10d.3～5min.25℃±2℃,pH7.0～8.5,、DO0、0～0.3、2～4mgL.1　Fig.1　SchematicdiagramoftheSBRusedinthisstudy1.2　,NaAc、NH4Cl、KH2PO4、NaNO3,,NaAc,NH4Cl,1.2.1.3　PAOs(1.0～　　　　　　1　Table1　CompositionofthesyntheticwastewaterNaAc(COD)NH4Cl(N)KH2PO4(P)NaNO3(N)300mgL13mgL10mgL5～10mgL1mL15L2　mg·L-1Table2　Compositionofthetraceelementsmg·L-1FeSO4·7H2O3000H3BO320MnSO4·4H2O26NiCl2·6H2O36CoCl2·6H2O50EDTA50CuCl2·2H2O7(NH4)6Mo7O24·4H2O21ZnCl2241.5mm,25～30mh)(14.6),AAO,COD,,,PAOsDNPAOs.,.1.4　(1)PHBBraunegg[15]　50mL(MLSS3000mgL),,3,20mL,(500W,30min),HACH.2mL3%2mLHACH,100℃240min,15minHACH.HACH20min,,0.45μm2μL(GC).GC(Trace2000)(FID),220℃,AgilentDB-FFAP(30m×0.32mm×0.25μm),190℃,110,.(2)TP　[16].(3)　Oehmen[17],[18].22558:SBR(4)　d=(4Aπ)12,,A;.(5)　(P-W)[(R-W)-(Q-P)]×F,P,W,R、4℃,Q4℃,F[19].(6)　(P)、、、、CODSVI[16].2　2.1　AAONP2N、P　　　　　　.,5mgL,NO-3-N19d0.61mgL,NO-2-N15d0.87mgL;10mgL,NO-3-N1mgL,NO-2-N3.7mgL,,51d0.08mgL,NOx-N1mgL.,PO3-4-P1mgL;60d,,,79.5mgL28.2mgL,35d25～33mgL.,60d,NOx-NP93%90%,　　　　　　2　NPFig.2　ChangesofNandPduringtheinduction25～33mgL,.2.2　PN,NOx-NP(3).18d,5mgL(2),NOx-N100%,16.6mgL,ΔPΔNOx-N,4.79,1.61,:ΔPΔNOx-N=(mg)NOx-N(mg)(1)　　10mgL,NOx-N93%,,13mgL,ΔPΔNOx-N,1.3.,PNO-3-N1.22[20].2256　　　　　　29,,.3　NOx-NPPNOx-NFig.3　ChangesofNOx-N,PandPNOx-Natanoxicstageduringtheinduction2.3　PHBTPAAO,4.,COD,,COD58mg(g·h)(VSS,),18.39mg(g·h)[AO68.5mg(g·h)],ΔPΔCOD0.32,:ΔPΔCOD=(mg)COD(mg)(2)Smolders[21]Filipe[22],0.470.55,ΔPHB-CODΔCOD0.5,:ΔPHB-CODΔCOD=PHB(mg)COD(mg)　(3)Smolders[21](PHB-COD)0.89mgmg.20min1.83mgL,3.43mgL,18.19mg(g·h),23.72mg(g·h)[AO36.8mg(g·h)],1.45mg(g·h),2.74mg(g·h),ΔPHB-CODΔP4.29,:ΔPHB-CODΔP=PHB(mg)(mg)(4)17.15mg(g·h),ΔPHB-CODΔP4.29,P4.82%(SS,),AOP(6%～9%).,ΔPHB-CODΔP0.20[23],(GAOs)PHB,20%(VSS,);,PHB..270mL,SS50mgL,SS3661mgL,VSSSS68%,4.31mgL,:TPinfluent=9.7mgL×2L×6d=116.4mgd(5),6.TPeffluent=3661mgL×0.27Ld×4.82%+4.31mgL×2L×6d=99.36mgd(6):TPinfluent=TPeffluent(7)4　SBRPHBTPFig.4　WaterqualityandthePHB&TPofsludgeduringtypicalcycleinSBR2.4　DNPAOs2,,DNPAOs;,non-DNPAOs.DNPAOs,non-DNPAOs22578:SBR,DNPAOs,DNPAOsPAOs[24～27].SBR,2,.5.,34.1mg(g·h),5.1mg(g·h),,PAOsDNPAOsPAOs14.9%[5(a)];,17.1mg(g·h),13.8mg(g·h),,DNPAOsPAOs80.7%[5(b)].211%64%[26],.1.7,DNPAOs;,0.5,.5　AOAAFig.5　PhosphorusuptakeunderAOandAAconditionswiththegranularsludge2.5　,1.0～1.5mm,29～51mh,94%,1.0470;SVI50mLg.[28],,0.16～0.7,0.0031.3　(1)AAODNPAOs.P90%,NOx-N93%,1gNOx-NP1.3g.(2)PAOs,PAOs.(3)DNPAOsAAOPAOsAO.(4),DNPAOsPAOs14.9%;AAODNPAOs80.7%.(5)1.0～1.5mm,29～51mh,94%,1.0470,SVI50mLg.:[1]　,.ASBR[J].,2003,19(8):40-43.[2]　.SBR[D].:,2006.[3]　PengDC,NicolasB,Jean-PhilippeD,etal.Aerobicgranularsludge-Acasereport[J].WatRes,1999,33(3):890-893.[4]　SungS,DanueRR.Laboratorystudiesontheanaerobicsequencingbatchreactor[J].WatEnvironRes,1995,67(3):294-301.[5]　PengDC,WangY,WangH,etal.Biologicaldenit