SRT对酵母SBR处理油脂废水稳定性的影响吕文洲

38420174ENVIＲONMENTALSCIENCEVol．38No．4Apr．2017SＲT-SBＲ*315211SＲTSＲT．SＲTSBＲSＲT5、10、20、40d、EPS、PCＲ-DGGE．SＲT5～10dSＲTCODEPSEPSEPS5～40dSＲTSVISＲT＞20d、SＲT3．SＲT．SＲT-SBＲX703.1A0250-3301201704-1513-07DOI10．13227/j．hjkx．2016071752016-07-262016-11-02LY12E08007/Y201121195/XKl15D2291974～[e-mail]*[e-mail]ofYeast-SBＲinTreatingOil-containingWastewaterLWen-zhouZHANGShu-linQIAOYu-xiangLIUYing*CollegeofArchitecturalCivilEngineeringandEnvironmentNingboUniversityNingbo315211ChinaAbstractSludgeretentiontimeSＲTisacrucialparametertoinfluencethestabilityofbiologicalwastewatertreatmentsystems．EspeciallytheeffectsofSＲTonyeast-wastewatertreatmentremainunclear．Inthisstudymixturesofyeaststrainswereappliedtotreatoil-containingwastewaterinsequencingbatchreactorsSBＲandtheeffectsofsludgeretentiontimeSＲTas5102040dontheremovalefficiencyofpollutantscontentsandcompositionofextracellularpolymericsubstancesEPSyeastcellssettleabilityandyeastcommunitieswereinvestigated．TheresultsshowedthattherecommendedSＲTwas5-10dfortheyeast-SBＲsystemHigherSＲTledtodecreaseofCODremovalrateandcontentofEPSthetightly-boundedEPSwasthemajoronewhichconsistedofpolysaccharides．SＲTof5-40dhadnosignificanteffectsontheSVIofyeastcellshoweverlongerSＲT＞20dresultedintheincreaseofmycelialcellsandatendencytoproducethefilamentousbulking．InthecontinuousoperationofSBＲthreeextraneousyeaststrainscapableofutilizingordegradingoilwereidentifiedinthesystemsundertheshortandlongSＲT．ToconcludeshorterSＲTwasfavorableforthesystemstabilityintreatingoil-containingwastewaterbyyeasts．KeywordssludgeretentiontimeSＲTyeast-SBＲextracellularpolymericsubstancesEPSmycelialcellssystemstability、、12．SＲT、、、34EPS56．EPS7～10．EPSEPS11．．SＲTEPSEPS．SBＲSＲTEPS、EPSEPSPCＲ-DGGE、SＲTSＲT．11.13O2Candidatropidalis、G1Candidalipolytica、W1Candidahalophila．12．38COD20000mg·L－1．COD6500～8500mg·L－1TN3.4～5.8mg·L－1TP32.4～45.4mg·L－1BOD51950～3200mg·L－11817～2258mg·L－1pH6.5～8.8．1.242LSBＲ．BOD5NH42SO4、KH2PO4BOD5∶N∶P100∶5∶1．9h3h1/2．pH5.0～5.528℃0.5～1.5mg·L－1．1.310LCOD3000～4000mg·L－1、BOD∶N∶P100∶5∶1pH5.0～5.5．4dCOD7500mg·L－1MLSS5.3g·L－1SBＲ．MLVSS/MLSS90.93%MLSS4.5g·L－1．SＲTSＲT5、10、20、40d．SBＲSＲTSＲT=40dSVI13．1.4CODDＲB200DＲ1010CODHACH5000r·min－15minCODOIL480BOD5BODTrakHACHpHHM-30PpHDKK-TOAMLSSMLVSS．TBEPSTBEPSLBEPS2LBEPS．LBEPS148000r·min－110min．0.9%NaCl5min12000r·min－115minLBEPS．TBEPS14LBEPS80℃30min12000r·min－115minTBEPS．-．1.51.5.1DNASBＲ1.5mL2mL8000r·min－12minFastDNASPINKitforSoilMpbioDNA．DNA－20℃．1.5.2PCＲ-DGGEPCＲGC1-NL15'-GCATATCAATAAGCGGAGGAAAAG-3'LS25'-ATTCCCAAACAACTCGACTC-3'．．50μLPCＲ1μLDNA1μL0.5μL23μLＲNAFreewater25μLPremixTakaraTaqVersion2.0plusdye．PCＲ95℃5min3095℃60s52℃45s72℃60s72℃7min．PCＲ1.0%．DGGEBio-Ｒad．8%30%～50%．PCＲ50μL6×loadingbuffer10μL35μL．60℃、120V6～8h0.01%SYBＲBiotium20min25minGelDocXＲBio-ＲadUSA15．1.5.316SrＲNADGGEPMD19-TTakara16℃16h－80℃EscherichiacoliDH5αTakara10μL42℃90s1～2minLB890μL37℃170r·min－11h．/IPTG/X-GalLB37℃16h．41514SＲT-SBＲ．GenBankhttp//．ncbi．nlm．nih．gov/BLAST/．22.1SＲTCODSＲTCOD1．SＲT20dCOD5dSＲT5dCODSＲT10d20d．SＲTF/MSＲT20dSＲTSＲT=5dSＲT．SＲT40d30dSＲT40d．SＲTSＲT40dSＲT．1SＲTCODFig．1EffectofSＲTontheCODandoilremovalrate2.2SＲTEPSEPS161718．Ｒavella19EPS．TBEPSLBEPSTBEPS、．EPSEPS70%～80%17．SＲTEPS20．SＲTLBEPS2LBEPSEPSSＲT．-1/θC=Y×Nrs－KdSＲT．Batstone21、22EPSSＲTSＲTF/MEPS23．2SＲT5d、10d4～5dLBEPSSＲTLBEPS．SＲT40d30d241bEPS515138．MatlabSＲT5、10、20、40dX1LBEPSX2α=0.000α＜0.01SＲTLBEPSSＲTLBEPS．2SＲTLBEPSFig．2EffectofSＲToncompositionandcontentofLBEPS3SＲTTBEPSFig．3EffectofSＲToncompositionandcontentofTBEPS3TBEPS．TBEPS2TBEPSLBEPS1013．TBEPSLBEPSSＲT．SＲT5、10、20d5dTBEPS．SＲT40dTB20dTBEPS．SＲT5、10、20、40dX1LBEPSX2α=0.00α＜0.05SＲTTBEPSSＲTTBEPS．2.3SＲTSVISＲT4SVI4．20dSVISVISＲTSＲTSVI13．18d30dSVISＲT．SＲTMLSSSVISV30SＲTMLSSSVISＲT．30dSＲT40dSVISＲTEPS2361514SＲT-SBＲMLSS．SＲT20dSVI5SＲTFig．5MicroscopyimagesofyeastcellswithdifferentSＲTinSBＲs．5SＲT．SＲT5dSＲT10、20、40dSＲT40d．MatlabSＲTSVIα=0.45SＲTSVISＲTSVI．SＲTSＲTSＲT．2.4SＲT5dSＲT40dPCＲ-DGGE6．DGGE3O2、G1、W1315．22d3．10dSＲT34SＲTSBＲSVIFig．4VariationofSVIofyeastcellswithdifferentSＲTinSBＲs1、2、3SＲT40d3．、GenBank1．Candidacylindracea100%2．Candidaintermedia99%3．Yarrowialipolytica99%1．3325～27．SＲT40d715138SＲTSＲT．Candidaalbicans28．SＲT．6SＲTDGGEFig．6DGGEimageofyeastcommunitywithdifferentSＲTinSBＲs116SrＲNATable1Comparisonofextraneousyeaststrainsby16SrＲNAsequences/%1DQ377633100CandidacylindraceastrainVTTC-045292AB27859999Candidaintermedia3EU23937999YarrowialipolyticastrainTJY18b31SＲT5～10dCODSＲTCOD．2EPSTBEPSEPSSＲTEPSSVI．3SＲT5～40dSＲTSVISＲT．4．1JarbouiＲBaatiHFetouiFetal．YeastperformanceinwastewatertreatmentcasestudyofＲhodotorulamucilaginosaJ．EnvironmentalTechnology2012338951-960．2DavereyAPakshirajanK．Treatmentofdairywastewatercontaininghighamountoffatsandoilsusingayeast-bioreactorsystemunderbatchfed-batchandcontinuousoperationJ．DesalinationandWaterTreatment201657125473-5479．3．J．2015283453-459．TianYDuanLSongYHetal．CharacteristicsofmicrobialcommunitystructureinmembranereactorsunderdifferentsolidretentiontimesJ．ＲesearchofEnvironmentalSciences2015283453-459．4SilvaAFAntunesSSaundersAetal．Impactofsludgeretentiontimeonthefinecompositionofth