SRT及碳源浓度对厌氧好氧交替运行SBR工艺中PHB的影响吴光学

SRT/SBRPHB,＊(,　100084):SRT/SBRPHB.:SBR51、SBR10SBR52PHBMLVSS9.8%、5.72%18.89%,20min46%、34%36.3%,PHB196.6mg/(L·h)、140mg/(L·h)295.35mg/(L·h);PHBPHB,;PHB;PHB.:/SBR;-β-;;:X703.1　:A　:0250-3301(2005)02-0126-05:2004-03-15;:2004-05-25:(50108007):(1979～),,,.＊EffectsofSRTandCarbonConcentrationonthePHBintheAnaerobic/AerobicAlternativeOperatingSBRProcessWUGuang-xue,GUANYun-tao(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China)Abstract:EffectsofSRTandcarbonconcentrationonthePHBoftheanaerobic/aerobicSBRprocessarestudied.TheresultsshowthatbytheorderofSBR51,SBR10andSBR52,themaximumPHBcontentofMLVSSwere9.8%,5.72%and18.89%,respec-tively,whilecarbontranslatingratioswere46%,34%and36.3%duringthefirst20minofaerobicphaseandPHBformingratewere196.6mg/(L·h),140mg/(L·h)and295.35mg/(L·h).PHBdegradationratecanbedescribedwithafirstorderdegradationratewithrespecttothePHBcontentofthecells.ReclamationofPHBshouldconsiderfromseveralfactors.Carbonconcentrationgra-dientisthemainfactorinfluencingPHBformationunderbatchexperiment.Keywords:anaerobic/aerobicalternativeSBRprocess;poly-β-hydroxybutyrate;sludgeretentiontime;carbonconcentration　　-β-(PHA,-β--β-,PHBPHV),,PHA,.PHA,PHA:/SBRPHA,;;[1].PHA,3(ASM3)PHA[2],Monod.,/SBR,/,(sludgeretentiontime,SRT)PHB(,PHB,PHAPHB,PHV),PHBPHB(MLVSS).1　1.1　(,6L)SBR:+(2h)+(4h)+(2h)++(4h);1mg/L,,;20℃;26220053　　　　　　ENVIRONMENTALSCIENCEVol.26,No.2Mar.,2005DOI:10.13227/j.hjkx.2005.02.026;3.6L.,2.0g/L,.,SBR51SBR525d,1,12;SBR1010d,1.3.,[3],,pH,1.1　/mg·L-1Table1　Syntheticwastewater/mg·L-154616762NH4Cl97K2HPO475MgSO4153CaCl227KCl117NaHCO350020　　SBR,PHB.20℃;3mg/L;pH7～8;0、1、23h2;20mg/L.2　/mg·L-1Table2　Nutritionconcentration/mg·L-1CCNCPCNP546546546546NH4Cl9797K2HPO475751.2　MLVSS、COD[4],COD0.45μm.PHB[5,6].40mg105℃,,1,2-2mL、2mL(1∶4)200μL(2.0g50mL),100℃4h,15min;4mL,20～30s,,PHB,,;PHB.1μLPHBGC14B/FID(Shimadzu,Japan)(30m,250μm,0.4μm).,2mL/min;1∶100;FID;60℃,4min,12℃/min220℃,6min;250℃,180℃.2　2.1　CODPHB3,SBR51、SBR52SBR10/SBR(2h4h)COD、PHBPHB,1、23.1　CODFig.1　CODconcentrationunderonetypicalcycle2　PHBFig.2　PHBconcentrationunderonetypicalcycle1～3,,CODPHB2h,CODPHB,PHB.1272　　　　　　3　PHBFig.3　PHBcontentunderonetypicalcycleSBR51SBR10PHB,SBR52PHB,PHB.PHB,SBR51PHB,(MLVSS0.87g/L,SBR101.34g/L),PHB;PHB.PHB,PHBPHB.2.2　PHBPHB,PHB,PHB、PHB,PHB,.,、　　SRT,PHB.PHB,SBR51、SBR10SBR52PHB85.22mg/L、76.48mg/L223.28mg/L,MLVSS9.8%、5.72%18.89%;20minPHB(COD,1.7)COD46%、34%36.3%,PHB196.6mg/(L·h)、140mg/(L·h)295.35mg/(L·h),PHB226mg/(g·h)、104.6mg/(g·h)250.3mg/(g·h).2SBR52PHB,PHB,PHBCOD29.5%,PHB181.41mg/(L·h),PHB153.7mg/(g·h).,SRT,,SRTPHB;,PHB,PHB,PHB,PHB,.,PHB,SRT,,PHB、PHBPHB,PHB,.PHB,.PHBMLVSSωPHB(PHB0)(4),SBR51、SBR10、SBR52ωPHB　　　4　PHBFig.4　ThemodellinesofPHBdegradation128　　　　　　26:ωPHB=8.9e-0.54t　　(R2=0.984)(1)ωPHB=5.9e-0.53t　　(R2=0.976)(2)ωPHB=16.5e-0.51t　　(R2=0.975)(3)　　,dωPHB/dt=-0.54×ωPHB、dωPHB/dt=-0.53×ωPHBdωPHB/dt=-0.51×ωPHB,PHBPHB,SRT,ωPHB.,PHB,PHB,PHB.2.3　PHB,PHB,PHB,,PHB,PHB;PHB.,PHB,5.5　PHBFig.5　PHBcontentunderdifferentnutritionconditions5,,PHB,PHB.、、4h,PHB35.4%、35.2%、38.3%35.6%,PHB.PHB,PHB,,,PHB,;PHB.2.4　PHBXv,CODSr,COD(PHBPHB),PHBωPHB,PHB(C4H6O2)COD1.48[7]1.7.,PHB,2、3PHB,PHBΔXvΔωPHB,:Sr=1.48ΔXv(1-ωPHB)+1.7XvΔωPHB+1.7ΔXvωPHB(4)　　,[8],PHB,PHB,ΔXv0,(5):Sr=1.7XvΔωPHB(5)　　,Xv,ΔωPHBSr,PHB;,,(4),.Sr,ΔωPHB,N、P,PHB,PHB.2.5　PHB,,/,,PHB.,,PHB、PHBPHB,PHB,.,PHB,,1292　　　　　　,PHB,PHB,.,、PHB,PHB,.3　(1)/,PHB,;PHB;PHB.(2)PHB,SBR51、SBR10SBR5246%、34%36.3%;PHB196.6mg/(L·h)、140mg/(L·h)295.35mg/(L·h),PHB226mg/(g·h)、104.6mg/(g·h)250.3mg/(g·h);PHBωPHB,.(3)PHB,,.(4),PHB;PHB,.:[1]　TakabatakeH,SatohH,MinoT,etal.Recoveryofbiode-gradebleplasticsfromactivatedsludgeprocess[J].Wat.Sci.Tech.,2000,42(3-4):351～356.[2]　GujerW,HenzeM,MinoT,etal.ActivatedsludgemodelNo.3[J].Wat.Sci.Tech.,1999,39(1):183～193.[3]　SatohH,IwamotoY,MinoT,etal.Activatedsludgeasapos-siblesourceofbiodegradableplastic[J].Wat.Sci.Tech.,1998,38(2):103～109.[4]　《》.()[M].:,1989.[5]　RiisV,MaiW.Gaschromatographicdeterminationofpoly-β-hydroxybutyricacidmicrobialbiomassafterhydrochloricacidpropanolysis[J].JournalofChromatography,1988,445:285～289.[6]　TakabatakeH,SatohH,MinoT,etal.PHA(polyhydrox-yalkanoate)productionpotentialofactivatedsludgetreatingwastewater[J].Wat.Sci.Tech.,2002,45(12):119～126.[7]　RittmannBE,McCartyPL.:[M].:,2002.[8]　DaiggerGT,GradyJrCLP.Thedynamicsofmicrobialgrowthonsolublesubstrates[J].WaterResearch,1982,16(4):365～382.()1)121890.84202100.72218910.96211540.99316920.79221310.83413860.97231270.97512230.95241090.9168600.94251070.9578310.95261030.9986340.9727840.5795450.9528780.9110·5300.9229690.92114680.9330610.92123620.9531560.85133220.8632440.87143190.7933390.89153080.9534370.80162950.9635320.89172840.7336320.84182680.9037170.88192110.953821.001)(),.(2002～2004).2004-12130　　　　　　26