UASB反应器对晚期渗滤液的碳氮协同削减效应赵琛

3211201911ＲesearchofEnvironmental[e-mail]．*[e-mail]．51678549“”SupportedbyNationalNaturalScienceFoundationofChinaNo．51678549SpecialFundingfor‘SpecializedExpertsinGuangxi’ofChinaUASB12233*1341．5410042．1000123．1000124．330031UASB+++UASB．①UASBρNOx－-NOＲPOＲP－300±10mVρCODCr313.00mgL106.00mgLρTN139.60mgL60.30mgL66.13%、56.81%．②、、、、．③16SrＲNA++UASBVadinHA17、Clostridium、Anaerolineaceae、Denitratisoma3.29%、35.17%、8.60%、2.84%．VadinHA17UASBClostridiumUASBClostridiumClostridiumNOx－-NAnaerolineaceaeDenitratisomaUASB．UASBVadinHA17、Clostridium、AnaerolineaceaeDenitratisomaCODCrTN66.13%、56.81%TN．UASBX7031001-6929201911-1913-08ADOI10.13198j.issn.1001-6929.2019.07.13TheSynergisticEffectforCNＲemovalbyUASBＲeactoronMatureLandfillLeachateZHAOChen12ZHANGLieyu2MATao3XIBeidou3*HUANGLiangliang1LIXiaoguang3YAOLei41．SchoolofEnvironmentalScienceandTechnologyGuilinUniversityofTechnologyGuilin541004China2．BasinＲesearchCenterforWaterPollutionControlChineseＲesearchAcademyofEnvironmentalSciencesBeijing100012China3．ＲesearchLaboratoryofSimulationandControlofGroundwaterPollutionChineseＲesearchAcademyofEnvironmentalSciencesBeijing100012China4．SchoolofＲesourcesEnvironmental＆ChemicalEngineeringNanchangUniversityNanchang330031ChinaAbstractInordertostrengthenthebiologicaltreatmentprocessthisresearchusedtheUASBreactorforthetreatmentofwastewaterandutilizedbiologicalprocessingtoachievesynergisticremovalofrefractoryorganicsandTN．Theresultsshowthat1ThisresearchoptimizedtheOＲPinmicroenvironmentbycontrollingρNOx－-NinUASBreactor．WhentheOＲPwas300±10mVρCODCrmainlyhumicacidfulvicacidandotherrefractoryorganicswasreducedfrom313.00mgLto106.00mgLρTNwasreducedfrom139.60mgLto60.03mgLandtheremovalratesreached66.13%and56.81%respectively．2Fourierinfraredspectrumanalysisshowedthatthefunctionalgroupsrelatedtocarboxylamideandbenzeneringwerereducedandthefunctionalgroupsrelatedtothealiphaticgroupwereincreased．316SrＲNAresultsrevealedthatthedominantstrainsofVadinHA17ClostridiumAnaerolineaceaeDenitratisomainthesystemreached3.29%35.17%8.60%2.84%respectively．VadinHA17coulduseavarietyofcomplexorganicsin32UASBreactorasthecarbonsourceinthedenitrificationprocess．ClostridiumactivatedtheextracellularoxidaseoutofthallusstrengtheningthedegradationabilitytorefractoryorganicstransferringtheintracellularelectronstotheextracellularelectronacceptorthroughthecytochromeonthecellmembranewhichconvertedNOx－-Nintoammoniaandreducedthenitrogencontent．AnaerolineaceaeandDenitratisomaactedintheanaerobicdigestionprocessandbiologicalnitrogenremovalprocessofUASBreactorrespectively．TheresearchshowedthatundertheactionofVadinHA17ClostridiumAnaerolineaceaeandDenitratisomaintheUASBreactortheremovalratesofCODCrandTNreached66.13%and56.81%respectivelyachievingtheaimofsynergisticremovalofrefractoryorganicsandTN．KeywordsUASBmaturelandfillleachatesynergisticeffectforCNremovaldominantstrains①ρNH3-NCNρTN1-3②ρBOD5ρCODCr0.1～0.24．56．7．．8、NH3-NTN63%ρCODCr60mgL40mgL．9TN．10-111.3～2mCODCrTN3∶1．WU2UASB1-AO-DUASBUASBNOx－-NCODCr、TN、NH3-N95%、98%、95%UASB1、AODUASBCODCr76.8%、2.8%、16.3%．UASBTN．++12．Silva-fcv13、、．NOx－-NTN．+UASB+NOx－-NUASB．11.1++UASBUASB1．UASB5L4.50cm8cm1.80m．LongerBT100-2J+YZ1515X2.10mLmin++UASB30～35℃UASBm312kg．60d2～4mm79.5%ρMLVSSρMLSS0.73．1.2+UASB1．UASB419111UASB1++UASBFig．1Thediagramofanaerobic-aerobic-UASBreactor．1.3ρCODCr、ρNH3-N、ρNO3－-N、ρNO2－-N《》．OＲPDQ30d．DOMdissolvedorganicmatter150W5nmλEx200～450nmλEm280～550nm5nm2400nmminPMT700V14．1+Table1Thewaterqualityoflandfillleachateofanaerobic-aerobicreactorρmgLCODCrTNNH3-NNO3－-NNO2－-NBOD54700.001229.751221.194.191.35230.00+313.00139.607.4030.40101.8042.00FouriertransforminfraredsepctrometerFT-IＲ++UASB1mm750～4000cm－1．UASB16SrＲNA16SrＲNArＲNADNA．16SrＲNA338F_806ＲF5'-ACTCCTACGGGAGGCAGCAG-3'Ｒ5'-GGACTACHVGGGTWTCTAAT-3'V3+V4．DNA、、PCＲ、PCＲ、PE、Illumina15．OTU97%MothurＲ．++UASB60d3Ld48hUASB3UASB、+CODCr、TN93.34%、88.32%．22.1UASB2+ρCODCr4700.00mgL313.00mgLρNH3-N1221.19mgL7.40mgL．、．NH3-N．UASBρNO2－-N、ρNO3－-N101.80、30.40mgL．UASBρNOx－-NOＲP．OＲPOＲP－300±10mV16．ρTN139.60mgL5191322+Fig．2Thechangeofcarbonandnitrogenconcentrationinanaerobic-aerobicreactor60.30mgL56.81%ρNO2－-N、ρNO3－-N101.80、30.40mgL41.30、15.50mgL75.40mgLρTN95.1%3．7NOx－-NN2．UASBρCODCr313.00mgL106.00mgL66.13%．NOx－-NOＲPOＲP++UASBNOx－-NTN．CODCr3UASBFig．3Thechangeofcarbonandnitrogenconcentration、、17-19．UASBCODCrUASB、4．4UASB1〔λExλEm=245nm445～450nm〕201500～1600UASBUASB2λExλEm=230nm340nm650～7503〔λExλEm=245nm445～450nm〕650～750UASBCODCr66.13%UASB．2.2UASBUASB．．5O—H、N—H3750～3000cm－13240～3550cm－1UASB、、、、、214UASBFig．4Three-dimensionalfluorescencespectrogramofUASBreactor619111UASB5UASBFig．5TheinfraredspectrogramofUASBreactor1900～1650cm－11755～1670cm－1．IＲ1755～1670cm－1、、、、、UASB7UASBFig．7TheabundanceofmajorbacteriainUASBreactor、X—HX—Y1475～1000cm－11250～1450cm－1UASB、、770～735cm－1710～690810～750cm－1830～810cm－118UASBUASB、、、、．UASB++UASB、、．Shannon-Wiener22．62000023．6Shannon-WienerFig．6ThecurveofShannon-Wiener259UASB460．++UASBUASB7．1%VadinHA1726.20%、FCPU