Zn硫化物和苯酚对短程硝化污泥活性的抑制刘滔

1110Vol．11No．10201710ChineseJournalofEnvironmentalEngineeringOct．201716YFXTSF003902017－01－222017－02－161991—。[e-mail]*[e-mail]Ⅱ、11*123311.3003842.4730043.100085Zn2+、、。Zn2+、23Zn2+。Zn2+、IC5038.29、139.133.46mg·L－18h7.46%、46.09%、22.69%。Zn2+Ｒ20.99720.9908-。X703.1A1673-9108201710-5341-10DOI10.12030/j．cjee．201701135InhibitionofpartialnitritationactivitybyZnⅡsulfideandphenolinacti-vatedsludgeLIUTao1CHIYongzhi1*FUHaijuan1SONGJianyang2ZHANGYu3TIANBinghui3FEIXuening11.TianjinKeyLaboratoryofWaterQualityScienceandTechnologySchoolofEnvironmentalandMunicipalEngineeringTianjinChengjianUniversityTianjin300384China2.SchoolofCivilEngineeringNanyangInstituteofTechnologyNanyang473004China3.StateKeyLaboratoryofEnvironmentalAquaticChemistryＲesearchCenterforEco-EnvironmentalSciencesChineseAcademyofSciencesBeijing100085ChinaAbstractZincZn2+sulfideandphenolfrequentlydetectedinindustrialwastewateraffectefficienciesofmanybiologicalindustrialwastewatertreatmentsystems．Thisstudyinvestigatedtheinhibitioneffectsandrecov-eryperformanceofZn2+sulfideandphenolonpartialnitritationsludgebybatchexperiments．ToinvestigatethecombinedsuppressionofZn2+andphenolaswellassulfidesandphenolonpartialnitritationsludgethetwofactorsandthreelevelsofresponsesurfacemethodologywasusedbasedonthecentercombinationdesign．TheresultsshowedthatthehalfmaximalinhibitoryconcentrationIC50ofZn2+sulfideandphenolonpartialnitri-tationsludgewere38.29139.13and3.46mg·L－1respectively．Therecoverypercentageoftheactivityofpartialnitritationsludgeafterremovingthethreeinhibitorswithin8hourswere7.46%46.09%and22.69%respectively．Themultiplecorrelationcoefficientofthetwobinaryquadraticresponsesurfaceregressionequationswere0.9972Zn2+andphenoland0.9908sulfidesandphenolrespectively．Theexperimentalresultsareingoodagreementwiththepredictedvaluesofthemodel．Itisshownthatthemodelcanprovidetheoreticalguid-ancefortheapplicationofpartialnitritation-anammoxtotreatwastewater．KeywordspartialnitritationinhibitionZn2+sulfidephenolcombinedsuppression11-、、1。-2-。nitrite-oxidizingbacteriaNOBammonia-oxidizingbacteriaAOB31∶1.32。-、C/N、、、。、、Zn2+、、、、4-6。AOB、pH、。-。7、8、9、1011、、。。Zn2+、。Zn2+222。11.11.1.1334d。8h1430min150mg·L－10.1～0.2mg·L－185%～95%55%～65%。1.1.2750mg·L－112-131。5。1Table1Compositionofsyntheticwastewater/mg·L－1/mg·L－1NH4HCO34232.14CuSO4·5H2O1.25NaHCO34500.00ZnSO4·7H2O1.30KH2PO465.81NiCl2·6H2O0.95FeSO4·7H2O9.16Na2MoO4·2H2O1.10H3BO40.05CoCl2·6H2O1.20MnCl2·4H2O4.95243510ZnⅡ、1.1.3Zn2+、3ZnSO4·7H2O、Na2S·9H2O。。1.21.2.1250mL。totalsuspendedsolidTSSvolatilesuspendedsolidsVSS1.79g·L－1、1.32g·L－1。50mL1250mL。150mg·L－118～25℃pH7.5～8.56～8mg·L－1。3.5h4h。1.2.238h。1.3、、、TSSVSS14、0.45μm。pHPONSELODEON。12ρ=Cs－C0/t·CVSS1ρmg·g·h－1Csmg·L－1C0mg·L－1thCVSSg·L－1。IＲ=ρ0－ρc/ρ0×100%2IＲ%ρ0mg·g·h－1ρccmg·L－1mg·g·h－1。1.4LuongAOB73。r=rmax·1－x/kin3rxmg·g·h－1rmaxmg·g·h－1xmg·L－1kiLuongmg·L－1nLuong。154。y=1－1/1+x/ab×100%4yx%xmg·L－1aIC50mg·L－1b。22.1Zn2+、、2.1.1Zn2+、、Zn2+、3435112。2Table2Concentrationsofvariousinhibitorsinindividualfactorexperimentsmg·L－1123456Zn2+02040608010007014020027035002468122.1.2Zn2+、、16。Zn2+1。Zn2+Zn2+100mg·L－129.08mg·g·h－13.38mg·g·h－188.36%2。Zn2+100mg·L－18h7.46%Zn2+。Zn2+。1Zn2+Fig．1EffectofZn2+concentrationonspecificammoniaoxidationactivityofpartialnitritationsludge2Zn2+Fig．2EffectofZn2+concentrationontheinhibitionofspecificammoniaoxidationactivityforpartialnitritationsludge3。30、70、140、200、270350mg·L－128.80、21.56、13.31、11.23、8.425.33mg·g·h－1。。40mg·L－10%25.14%、53.77%、61.01%、70.75%81.50%。AOBAOBAOB17。8h46.09%50%。5。3mg·L－1443510ZnⅡ、12mg·L－129.08mg·g·h－11.99mg·g·h－10mg·L－160%93.16%。。AOBAOBAOBpHAOB18。8h22.69%。3Fig．3Effectofsulfideconcentrationonspecificammoniaoxidationactivityofpartialnitritationsludge4Fig．4Effectofsulfideconcentrationoninhibitionofspecificammoniaoxidationactivityforpartialnitritationsludge5Fig．5Effectofphenolconcentrationonspecificammoniaoxidationactivityofpartialnitritationsludge6Fig．6Effectofphenolconcentrationoninhibitionofspecificammoniaoxidationactivityforpartialnitritationsludge2.1.3Origin9.0Luong1、3、5Zn2+、、Luong3。3Zn2+、29.35、29.0329.28mg·g·h－129.08、28.8029.08mg·g·h－1Ｒ20.9854、0.97130.984735435113。Origin9.0Zn2+、4。3LuongTable3FittingresultsofLuongkineticmodelrmax/mg·g·h－1ki/mg·L－1nＲ2Zn2+r=29.35×1－x/112.300.6929.35112.300.690.9854r=29.03×1－x/472.290.6029.03472.290.600.9713r=29.28×1－x/12.710.5529.2812.710.550.98474Table4Fittingresultsofmodifiednon-competitiveinhibitionmodela/mg·L－1bＲ2Zn2+y=100×1－1/1+x/38.291.8638.291.860.9935y=100×1－1/1+x/139.131.49139.131.490.9930y=100×1－1/1+x/3.461.643.461.640.98464Zn2+IC5038.29mg·L－1。CECEN19Zn2+IC5022.75mg·L－1Zn2+、、。NOB。CECEN19AOBNOBAOBNOBNOBZn2+IC50。4IC50139.13mg·L－1。206.20mg·L－13d50%、20VSS0.15g·L－13d。4IC503.46mg·L－1。21AOB2.61mg·L－1。2.2、Zn2+25、Zn2+Table5FactorsandlevelsofresponsesurfaceexperimentofphenolandZn2+mg·L－1－101A36.5010Zn2+B1537.5602.2.122.1Zn2+2235。2.2.2A、Zn2+BY。6。643510ZnⅡ、6、Zn2+Table6DesignandresultofresponsesurfaceanalysisofphenolandZn2+AB/%10080.5621190.0131－189.6340079.6450080.336－1.414051.8370078.418－1176.579－1－142.151001.41488.09111.4