白腐菌对焦化废水中吲哚的降解及其机理任大军

34　52006　5　　　　　　　　()J.HuazhongUniv.ofSci.&Tech.(NatureScienceEdition)Vol.34No.5　May　2006:2005-05-09.:(1978-),,;,(430074).E-mail:[e-mail]　():(104250).任大军a　张晓昱b　颜克亮b　陆晓华a(华中科技大学a环境科学与工程学院;b生命科学与技术学院,湖北武汉430074):BP,,、、pH.99%;,,97%;,,;BPpH6～7,pH5～8;HPLC3～6min.　　:;;:X172　　:A　　:1671-4512(2006)05-0121-04DegradationofindoleinwastewaterfromcokingplantsbywhiterotfungiRenDajun　ZhangXiaoyu　YanKeliang　LuXiaohuaAbstract:Whiterotfungus(BP)wasselectedtodegradeindole.Studiedwerethedegradationprocessandmechanismofindoleattwoconcentrationlevelsindifferentculturemediums,therelationshipbe-tweendegradationofindoleandactivityoflaccase,thebiomassandpHvaluesofculturemediums.Theresultsshowedthatmore99%ofindolewasdegradedbywhiterotfungiindifferentcultureme-diums.Thedegradationrateofindolewascorrelatedcloselywiththeactivityoflaccase.Whentheac-tivityoflaccasereachedmaximum,more97%ofindolewasdegradedbywhiterotfungi.Indoleswithhighconcentrationcouldinhibitethegrowthofwhiterotfungiandexcitetheproductionoflaccase.Theculturemediumofstrawleachingliquidcouldpromotethegrowthofwhiterotfungiandtheac-tivityoflaccase.TheoptimizedpHvalueofwhiterotfungus(BP)was6～7.IndolecouldbedegradedeffectivelywhenthepHvaluewasbetween5～8.Thenewsubstancesdetectedonthehighperform-anceliquidchromatogrambetween3～6minmaybeoxindoleandisatin.Keywords:whiterotfungi;cokingwater;indoleRenDajun　DoctoralCandidate;CollegeofEnvironmentalSci.&Eng.,HuazhongUniv.ofSci.&Tech.,Wuhan430074,China.　　,、[1],,.,,、、[2].,.[3],,,;、、pH,,DOI:牨牥牨牫牪牬牭牤jhust牪牥牥牰牥牭牥牫牳[4].BP,.1　1.1　BP,.250mL(100mL),25℃,150r/min4d,.1.2　:(1000mL),(10g),(1g),KH2PO4(1g),K2HPO4(0.1g),MgSO47H2O(0.24g),NaCl(0.01g),CaCl22H2O(0.1g),MnSO4H2O(0.08g),FeSO47H2O(0.1g),ZnSO47H2O(0.01g),CuSO45H2O(0.02g),VB1(0.01g).:(1000mL),(20%),(2%):(1000mL),(3%),(0.8%),(0.12%).1.3　,1501mg/L.,,.1.4　10mL100mL250mL,,84mg/L230mg/L,(150r/min),25℃.1.5　:HitachiHPLC(),HitachiL-7100Hitachi,UV-visL-7420HypersilC-18(250mm×4.6mmI.D.,5μm),[5].:(1%,80∶20),0.6mL/min,270nm,20μL.:UV-754,M.H.Gold[6].mLmin0.001.pHpHS-25pH.2　2.1　1(,“”ρ=84mg/L;“”ρ=1　230mg/L,),、、99%.,(1),1,0～9d,、,k01.8mgL-1h-1,[4];,,,,2.0mgL-1h-1,1　Ck0/(mgL-1h-1)r2()-1.8048t+1.89901.80480.9742()-1.8059t+2.01101.80590.9734()-1.8155t+2.13251.81550.9639()-2.0186t+1.67652.01860.9808()-2.0836t+2.40452.08360.9844()-2.0762t+2.52502.07620.9691122　　　　　　　　　()　34,[7],0～3d,94%.2.2　2.2.1　2,,3d6d;,0～3d,,6d.2　.,0～6d,3,,,97%;,0～3d,,,,94%,6d,97%.9d,,.2.2.2　3,,0～6d(3　3d),6～9d,,9d,,,,,.,,,,.,,.2.2.3　pHpH,4,,pH.4　pH,、pH5.25～6.25,6.50～8.286.15～6.80;,、pH5.41～6.42,6.25～7.916.19～7.96.,pH,、,pH5.90(6.23),6.50(7.14),6.32(6.58).BPpH6～7,pH5～8.2.3　HPLC5,5.51,6.5min.523,6d,,HPLC,3～6min,,.0～6d,3～5min5.2min,6～15d,.BP,H2O2OH,OH,,[4].[8],,,23,,,1235　　:　　5　HPLC　1—;2—6d;3—15d23,.,4.5min5.2min、,———.3　a.9d,99%;b.,,97%;c.,,;d.BPpH6～7,pH5～8;e.HPLC3～6min,.[1],　,.[J].,2002,33(4):39-42.[2],.[J].,2001,23(4):39-41.[3]KennethMG,HammelE.Initialstepsinthedegra-dationofmethoxychlorbythewhiterotfungusphan-erochaetechrysosporium[J].ApplEnvironMicrobi-al,1997,63(3):1175-1177.[4],　,.[J].,1999,86(3):8-12.[5]MohanS,Sistla,GuruR,etal.Microbialdegrada-tionofpyridineusingPseudomonassp.andisolationofplasmidresponsiblefordegradation[J].WasteManagement,2003,23,167-171.[6]GoldMH,WariishiH,BourbonnaiaR,etal.Dem-ethylationdelignificationofkraftpulpbytrametesversicolorlaccaseinthepresenceof2,2-azinobio-(3-ethylbenthiazoline-6-sulphonate)[J].ApplMicrobiolBiotechnol,1992,36:823-827.[7]YadavJS,ReddyCA.Degradationofbenzene,tolu-ene,ethylbenzeneandxylenes(BTEX)bythelignin-degradingbasidiomycelephanerochaetechrysospori-um[J].AppliedandEnvironmentalMicrobiology,1993,59(3):756-762.[8],,,.[J].,2003,22(2):170-173.46,(Christ-church)(GarryMoore)(Da-vidson).,,.“(HUST)(UC)”..,1873,,.124　　　　　　　　　()　34