苯胺废水SBR工艺生物强化处理效能

42620106JOURNALOFH[e-mail]、、、.100%COD93%.、..PCR－DGGE16、.PCR－DGGEX703.1A0367－6234201006－0949－05TreatmenteffectivenessofbioaugmentationonanilinewastewaterwithSBRWANGZheWEILiMAFangZHAOGuangZHANGSiStateKeyLaboratoryofUrbanWaterResourceandEn[e-mail]-degradingbacteriaconservedbylaboratoryweretestedinSequencingBatchReactorSBRtoinvestigatethecapabilityofanilineremovalthevariationofinterrelatedindexsuchasCODNH+4NO－2andNO－3andthecommunitysuccessionofmicroorganism.TheresultsshowedthatSBRstartedupquicklyanddegradedanilineeffectively.Theremovalefficiencyofanilinereached100%andtheremovalefficiencyofCODwasabout93%whenthesystemreachedsteadyrunning.ThephotographsscreenedbyscanningelectronmicroscopeSEMindicatedthattheaniline-degradingbacteriaspreadovertheactivatedsludge.ItwasfoundfromthePCR-DGGEmapandthesimilaritymatrixthatthespeciesandtheamountsofmicroorganismchangedgreatlyanddominantspeciesinthesystemwerestrengthened.Keywordsbioaugmentationaniline-degradationcommunitysuccessionPCR-DGGE2010－03－01.50908063NQQQ923245471987—1963—.、、1.2.、、、、、3.、、4－6..7－9.10JH－9SBR12℃174mg/L97.8%.SBR.11.11.1.111.1.1.2.1.1.30.5g/LNH4Cl0.025g/LKH2PO4、K2HPO40.005g/LCaCl20.042g/LMgSO4·7H2O0.06g/LNaH-CO30.13g/L1mL/L10g/LpH7.0.1.212.CODCL－12N－1－－pHpH.1.3SBR2L.1.%1.曝气泵；2.流量计；3.取样口；4.曝气头；5.加热棒；6.水泵56412311.412h10h2h25℃4/51/5.16.、、、、COD.1.5.、JH－9Acineto-bacter-calcoaceticus11.JH－9SBR.1.6PCR－DGGEDNAPBSbufferDNA.DNA－20℃.PCR－DGGEF338GC5’－CGCCCGC-CGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGACTCCTACGGGAGGCAGCAG－3’R5185’－ATTACCGCGGCTGCTGG－3’.PCRPCR95℃5min94℃30s56℃45s72℃2min3072℃15minPCR2%.DGGEBio－RadDcodeTMPCR.6%～12%30%～60%40%～60%100%7mol/L40%.PCR6μL6μL.60℃110V9h..ImageScannerII.22.12.2121.530%·059·42.57100%..9100%16...140120100806040200苯胺质量浓度/（mg/L）进水出水去除率100806040200苯胺去除率/%246810121416周期2SBR2.2、、、、3.6050403020100氨氮、亚硝氮、硝氮质量浓度/（mg/L）进水氨氮质量浓度出水氨氮质量浓度出水亚硝氢质量浓度出水硝氮质量浓度246810121416周期3SBR、、3NH+4.、.5530%.5.1033.6mg/L10100%.11.1631.8mg/L.3、.、.、.2.3CODCOD4.SBRCODCOD1000mg/L.3COD5COD.7CODCOD200mg/L80%.COD16COD100mg/LCOD92.23%.COD.CODCOD.·159·6SBRCOD.16001400120010008006004002000COD质量浓度/（mg/L）进水出水去除率1009080706050403020100COD去除率/%2468100121416周期4SBRCOD2.455000.（a）W2（b）W6（c）W1555.W22.W6680%.W1515100%、.2.5PCR－DGGE.W2、W4、W6、W8、W15、、、.6.6PCR－DGGE6.QuantityOne/DGGEDicecoefficient.6W61.·259·421%123451100.064.665.457.857.5264.6100.061.461.656.4365.461.4100.059.756.4457.861.659.7100.075.1557.556.456.475.1100.014W85W1575.1%.31SBR72h80%120h100%.CODCOD100mg/L92.23%..210120h.、.3...1.J.20061026－30.2ITOHNNAOKIM.TOYOKOK.Oxidationofanilinetonitrobenzenebynonhemebromoperoxidase.BiochemJ.MolBiolIntl199329785－791.3GMEZJLLENGHIDALGOAMetal.Applica-tionofreverseosmosistoremoveanilinefromwastewaterJ.Desalination2009245687－693.4CHENShengSUNDezhiCHUNGJong-shik.Anaero-bictreatmentofhighlyconcentratedanilinewastewaterusingpacked-bedbiofilmreactorJ.ProcessBiochem-isty2007421666－1670.5CHENShengSUNDezhiCHUNGJong-shik.Simulta-neousmethanogenesisanddenitrificationofanilinewastewaterbyusinganaerobic-aerobicbiofilmsystemwithrecirculationJ.JournalofHazardousMaterials2009169575－580.6LEICaihongJINZanfangPANZhiyan.Studyontreat-mentofanilinewastewaterbypressurizedactivatedsludgeprocessJ.JournalofZhejiangUniversityofTechnology2008364423－6.7HUBANC.MetalbioaugmentationputmicrobestoworkJ.ChemicalEngineering1997104374－84.8.J.2008285885－891.9YUFangboALISWGUANLiboetal.Bioaugmenta-tionofasequencingbatchreactorwithpseudomonaspu-tidaONBA-17anditsimpactonreactorbacterialcom-munitiesJ.JournalOfHazardousMaterials20101761－320－26.10.SBRJ.200934577－580.11.J.2007283241－245.12.M....1989.·359·6SBR