苯乙烯生物滴滤塔的工效和生物膜微群落吴献花

吴献花1　邵　丹1　林　洪1　孙石2　王树坤2　雷艳梅2①(1.,653100;2.,650093)[]苯乙烯;生物降解;生物滴滤塔工效;微生物分析[　]采用经培养驯化的污泥菌种和类球形陶粒等构建生物滴滤塔,评价了气体苯乙烯浓度、气体流量、循环液喷淋量对生物滴滤塔工效的影响,并对生物膜微群落中的微种群作了定性定量检测,通过与国外最新研究成果的对比,证实了项目研究结果结论的正确性。[]X701[]A[]1009-9506(2005)06-0084-06、、、。500℃60mg;、、、。。,、、、,,,。,189,(TLVs):(TLV-TWA)0.085gsm-3;(TLV-STEL)0.17gsm-3;8h50ppm。,,、,。。一、材料和方法(一)实验装置90mm,1500mm,、。2.5cm(121℃,60min),3.2L×2;,,(10mL)、(2mL)1(MSS1,90mL)[1],30℃48h。MSS1K2HPO41.55g,NaH2PO42H2O0.85g,(NH4)2SO42.0g,MgCl26H2O0.1g,EDTA10.0mg,Zn-SO47H2O2.0mg,CaCl22H2O1.0mg,FeSO47H2O5.0mg,Na2MoO42H2O1.0mg,CuSO45H2O0.2mg,CoCl26H2O0.4mg,MnCl24H2O1.0mg,1L,pH7.0。1mL150mLMSS130℃、120(120spm)48h150mL(NB)30h,10000rpm(1600g)20min,MSS1(NBMSS11～2),30℃24h。,1～3mm。1。84(21)20056　JournalofYuxiTeachersCollegeVo.l21No.6Jun.2005①[]2005-05-11[]吴献花(197l-),女,云南大理人,硕士,副教授,主要从事环境生态学与环境工程学教研工作。　[](863)(2002AA649050)2(MSS2)(g/L)K2HPO44.3,KH2PO43.4,(NH4)2SO42.0,MgCl26H2O0.34,1mL,H2O1000mL;(g/L):CaCl22H2O2.6,Na2SO42H2O2,MnCl24H2O1,FeSO47H2O0.6,pH6.8。1　　　　　　1.　2.　3.　4.　5.　6.　7.　8.　　　　　　9.　10.　G.　L.(二)试验方法,MSS2,(8)(5),,(6),(7)(8)。2-、,()0.1NNaOHMSS2pH6.8±0.2。,(1)(2),,(4)90%,,,CO2H2O,。(23±3℃),5.2～24.0Lh-1;200～600Lh-1,0～1.5gm-3,0200gm-3h-1;100～230Pa。(三)分析方法1.气体、液体样品苯乙烯浓度的测定;。、,、。2.生物滴滤塔工作效率(RE;%)(EC;gL-1h-1),:RE=(1-Cout/Cin)×100%EC=(Cin-Cout)Q/V-(Sout-Sin)L/VCin(mgL-1);Cout(mgL-1);Q(Lh-1);L(Lh-1);V(L);Sin(mgL-1);Sout(mgL-1)。　　3.生物膜微群落分析1g9mL0.2g1.8mL30min,1:10,10-2、10-3、10-4、10-5、10-6。,(CFUg-1);、,85:(dominantmicropopulation)。(NA,Qxoid,CM3,England)、(BHIB,Bi-omerieux,France)、(PAB,Oxoid,CM559,England)、(PCA,Merck,Germa-ny);1/10PCA、(BHIA)Bacto(Difco,LOT3069272),Vitek32GNI+、GPI、NFC、BAC。二、结果与讨论(一)进口气体苯乙烯浓度对生物滴滤塔工效的影响200Lh-110Lh-1,。2,,。500mgm-3,99%;1000mgm-3,90%。3,1500mgm-3,,,,。,1500mgm-3,35mgL-1h-1,,(individualabsorptionequilibrium)。:。　　(二)气体流量对生物滴滤塔工效的影响,,,,。45,600mgm-3,,,。200Lh-1600Lh-1,129.06s43.02s,99.46%69.73%,18mgL-1h-1。,、,;,。,,。86　　(三)循环液喷淋量对生物膜工效的影响,。6、7,,,。,。,,。(四)生物膜微群落,,(microcommunity),,。,,。NA、BHIA、PAB、PCA1/10PCA,、。、,。1、2、。1　35℃(CFU/g)24h48h72h120h(CFU/g)NA2.1×1072.5×1084.8×1084.8×108　BHIA1.2×1081.8×1083.48×1083.5×108　PAB3.0×1061.2×1086.2×1086.2×108　PCA7.4×1072.5×1088.5×1088.5×1088.5×1081/10PCA1.3×1082.0×108　　　1、2,,、、、、、,(abundance)、(diversity)(stability)。,、。(climaxcommunity)(homeostasis),。,(micro-eubiosis),。、、、,(colonizationresistance)。87:2　　35℃72h(CFU/g)NABHIAPABPCA1/10PCA(CFU/g)(Pseudomonasputida)2.5×1042.6×1043.1×1042.8×1045.5×1075.5×107(Pseudomonasfluorcens)1.0×1041.0×1041.5×1041.0×1041.5×104(Bacillusfusiformis)4.0×1043.5×1048.0×1045.0×1042.6×1062.6×106(Bacilluscereus)3.5×1044.0×1046.0×1045.2×1046.0×104(Acinetobacterlwoffii)3.6×1063.6×106(Acinetobacterjunii)8.0×1041.5×1051.5×105　　(五)其它相关研究,。Juneson,,,。50%～70%(WW/),120s、60s30s,1～3、4～610～16mm,350gm-3h-1,69～118gm-3h-1,65%～75%,100%[2]。Zilli,4∶1,(RhodococcusrhodochrousNCIMB13259),5cm、65cm,50cm,0.98L。,,0.10gm-3,0.10～1.0gm-37×107CFUg-1,1.0gm-3。0.80gm-3245mh-1,63gm-3h-1[3]。Jang,Pseudomonassp.SR-5,5cm、37cm()17cm(),236gm-3h-181gm-3h-1,CO290.4%36.7%;170gm-3h-1,95%;,[4]。,、、、,,(Pseudomonas)、(Rhodococcus)、(Xanthobacter)、(Corynebacterium)、(Enterobacter)、(Nocardia)[5]。,,/,、pH、、,。、、,、88。三、结　论1.、。200Lh-1,1000mgm-3,90%,30mgL-1h-1;600Lh-1,600mgm-3,70%,32mgL-1h-1。,35mgL-1h-1。。2.,。。3.,。、,。,、、,、、、、、pH、,,。4.,、。,。5.。[参考文献][1],,.[M].:,2004.[2]JunesonC,wardOP,SinghA.Microbialtreatmentofastyrene-contaminatedairstreaminabiofilterwithhigheliminationca-pacities[J]JIndMicrobiolBiotechno.l2001,26:196～202.[3]ZilliM,ConvertiA,FeliceRD,MacrokineticandquantitativemicrobialinvestigationonaBench-scalebiofiltertreatingstyrene-pollutedgaseousstreams[J].BiotechnologyandBioengineering,2003,83(1):29～38.[4]JangJH,HiraiM,ShodaM.StyrenedegradationbyPseudomonassp.SR-5inbiofilterswithorganicandinorganicpackingma-terials[J].EnvironmentalBiotechnology.SpringerLinkFull-TEXTARTICE,2004,May,8.[5],,.[J].,2004,14(6):79～82.PerformanceandBio-filmMicro-communityofBio-tricklingFiltertreatingStyrene-PollutedGaseousstreamWuXiang-huaeta.l(DepartmentofChemistry,YuxiTeacher’sCollege,Yuxi653100)KeyWords:styrene,biodegradation,biotricklingfilterperformance,microbialanalysesAbstract:Buildbiotricklingfiltertreatingstyrene-pollutedgaseousstreamadoptingenrichedsludgebacteriaandperlite.Theexperimentaldataofstyreneconcentrationinthegaseousstream,gasvelocity,liquidflowinrecir-culationcircuitwereusedtoevaluatetheinfluenceofstyreneloadingonbiotricklingfilterperformance.Qualitativeandquantitativemicrobialdataonmicropoputationsofbiofilmmicro-communitywerealsodetected.Reviewthenewachievementsinscientificresearchfromothercountries,confirmedtheresultsandconclusionsofthestudywerecorrec.tSuggestthemajorresearchareaandimportantproblemswhichhavetobedealtwith.89: