不同恢复方式对硝化颗粒污泥活性的影响郭秀丽

3410201310ENVIRONMENTALSCIENCEVol．34No．10Oct．2013112*11．1500402．150090SBR．SOURO2/VSSSOUR13.15mg·g·h－120dSOUR1.26mg·g·h－1595%13.87mg·g·h－1．30dSOUR11.63mg·g·h－15192.64%14.92mg·g·h－1SOUR．SOUR．20、30、40mg·L－140mg·L－13．1、2、3h1h．X703.1A0250-3301201310-3981-052013-01-192013-03-27211770331987～[e-mail]*[e-mail]ecoveryMethodsontheActivityofNitrificationGranularSludgeGUOXiu-li1GAODa-wen12LUJian-cong11．SchoolofForestryNortheastForestryUniversityHarbin150040China2．StateKeyLaboratoryofUrbanWaterResourceandWaterEnvironmentHarbinInstituteofTechnologyHarbin150090ChinaAbstractAerobicnitrifyinggranulesludgecultivatedinsequentialbatchreactorSBRwasusedtoinvestigatethecriticalactivitypointofgranulesandtheeffectofdifferentammoniaconcentrationandaerationtimeonreactivationafterstorage．TheresultsshowedthattherewasbigdifferenceintheactivitySOURO2/VSSofnitrifyingbacteriaafterdifferentstoragetime．ThespecificoxygenutilizationrateSOURofgranulesbeforestoragewas13.15mg·g·h－1．Afterastorageperiodof20daystheSOURdecreasedby1.26mg·g·h－1after5cyclesofreactivationtheammoniaremovalefficiencywasalreadyincreasedto95%whiletheSOURwasrecoveredto13.87mg·g·h－1．Butafterastorageperiodof30daystheSOURdecreasedby11.63mg·g·h－1after51cyclesofreactivationtheammoniaremovalefficiecnyonlyreached92.64%whiletheSOURwasrecoveredto14.92mg·g·h－1．Meanwhilethisstoragemethodrequiredalongerrecoverytime．ThereforeweputforwardthatthecriticalactivityofdenitrifyinggranularsludgeshouldbetheactivitywhenactivityrecoverystartsandthenitrifyingbacteriaSOURbeginstodecline．Onthebasisofthecriticalactivitywebegantorestoretheactivitywhentheactivityofthedenitrifyingbacteriawasreducedtocriticalactivityandthenstartedanewstoragecycle．Thisstoragemethodwasnameddynamicstorage．Differentinfluentammoniaconcentrationsof2030and40mg·L－1wereappliedtoreactivatetheaerobicgranules．HighestSOURcouldbeachievedwhenfedwithanammoniaconcentrationof40mg·L－1afterreactivation．AfterthreetimesofdynamicstoragetheSOURremainedstable．Differentaerationtimeof12and3hwasappliedtoreactivatetheaerobicgranules．HighestSOURcouldbeobtainedwhenaerationtimeof1hwasappliedafterreactivationandremainedstablealongwithdynamicstorage．KeywordsaerobicnitrifyinggranulesdynamicstorageactivityrecoveryspecificoxygenutilizationratesSOURaerationtime．、、、、12．COD、345．、．34．226．810d78．．、910．．4℃710d11．265d4h6h12．1314．．．11.11.21．11Table1Compositionofsyntheticwastewater/mg·L－1/g·L－1COD150～180FeCl31.5NH+4-N40H3BO30.15PO3－4-P5～6CuSO40.03290～320KI0.18CaCl210MnCl20.12NaMoO40.06ZnSO40.12CoCl20.15EDTA1011mL·L－11Fig．1Cultivationofdenitrifyinggranularsludge1.212L1LSBR12L60cm22cm1L44cm7cm．．12L．3h1min5min．1L21～5min5min．2Table2Experimentaldesign/mg·L－1/h12032303340344015402640474051.3COD、NH+4-N、NO－3-N、NO－2-N、MLSS、SV15．EPS16-17Lowry18．DO、pH、WTW．SOUR19．1.4289310SBRMLSS4500～5000mg·L－1．5250mL15、20、25、30、35d．72．SOUR．22.122．40～48mg·L－12mg·L－195%．、、．2Fig．2Denitrificationefficiencyofthegranularsludgebeforestorage2.215、20、25、30、35d3．SOUR13.15mg·g·h－115dSOUR13.07mg·g·h－120dSOUR11.89mg·g·h－125dSOUR4.27mg·g·h－130d1.52mg·g·h－135d1.41mg·g·h－1．15d220d5SOUR13.87mg·g·h－125d37SOUR13.52mg·g·h－130d5114.92mg·g·h－1．35d3940%35d．、20～22．．3SOURFig．3Changesinactivityofthenitrifyingbacteriawithdifferentstoragetime2.3、2324．．．0dSOUR13.15mg·g·h－127dSOUR11.31mg·g·h－1420mg·L－13297%．30、40mg·L－132100%．20、30、40mg·L－1389334．20、30、40mg·L－11SOUR11.85、12.79、13.72mg·g·h－1．255dSOUR10.29、10.34、10.98mg·g·h－1．56d40mg·L－1SOUR12.69mg·g·h－1．383d84d40mg·L－1SOUR13.08mg·g·h－1．40mg·L－1．．4SOURFig．4DifferentwaternitrogenconcentrationontheinfluenceofnitrifyingbacteriaSOURinactivationprocess2.4SBR1、2、3h6h1h62h33h2．1h360%2h382%3h399%．1hSOUR515.86mg·g·h－12h3hSOUR13.2213.72mg·g·h－1．/1h．1h25．5SOURFig．5EffectofdifferentaerationtimeontheSOURofnitrifyingbacteriaduringtheactivationprocess31．240mg·L－1SOUR13.15mg·g·h－1313.08mg·g·h－1．31hSOUR13.15mg·g·h－113.71mg·g·h－1．1．J．2009295449-473．2BartraliACarreraJPérezJ．Bioaugmentationasatoolforimprovingthestart-upandstabilityofapilot-scalepartialnitrificationbiofilmairliftreactorJ．BioresourceTechnology201110254370-4375．3．J．2003234380-384．4SuKZYuHQ．Formationandcharacterizationofaerobicgranulesinasequencingbatchreactortreatingsoybean-processingwastewaterJ．EnvironmentalScienceandTechnology20053982818-2828．5．J．201026489310410-14．6ZhangLLZhangBHuangYFetal．Re-activationcharacteristicsofpreservedaerobicgranularsludgeJ．JournalofEnvironmentalSciences2005174655-658．7GaoDWYuanXJLiangH．ReactivationperformanceofaerobicgranulesunderdifferentstoragestrategiesJ．WaterResearch201246103315-3323．8YuanXJDaWGHongL．ReactivationcharacteristicsofstoredaerobicgranularsludgeusingdifferentoperationalstrategiesJ．AppliedMicrobiologyandBiotechnology20129451365-1374．9LiuYYangSFTayJH．Improvedstabilityofaerobicgranulesbyselectingslow-growingnitrifyingbacteriaJ．JournalofBiotechnology20041082161-169．10WangXHZhangHMYangFLetal．ImprovedstabilityandperformanceofaerobicgranulesunderstepwiseincreasedselectionpressureJ．EnzymeandMicrobialTechnology2007413205-211．11WangXHZhangHMYangFM