不同接种物启动AnammoxEGSB反应器的污泥性能丁爽

　62　5　　　　　　　　　　　　Vol.62　No.5　20115　　CIESC　Journal　　　May　2011Anammox-EGSB丁　爽,郑　平,唐崇俭,张吉强,胡安辉(,310058):(AMS)、(FAS)(SAS),(Anammox-EGSB)。,3Anammox-EGSB:FASSASAMS;3(VSS/SS),,,,,Anammox,400～420nm。,Anammox。:;;;DOI:10.3969/j.issn.0438-1157.2011.05.032:X703　　　　　　:A:0438-1157(2011)05-1395-07CharacteristicsofanaerobicsludgesinAnammox-EGSBbioreactorsstartedwithdifferentinoculaDINGShuang,ZHENGPing,TANGChongjian,ZHANGJiqiang,HUAnhui(DepartmentofEnvironmentalEngineering,ZhejiangUniversity,Hangzhou310058,China)Abstract:ThecharacteristicsoftheanaerobicsludgesinAnammox-EGSBbioreactorsstartedupwithanaerobicmethanogenicsludge(AMS),freshAnammoxsludge(FAS)andstoredAnammoxsludge(SAS)wereinvestigatedrespectively.ItwasfoundthatAnammox-EGSBbioreactorscouldbestartedupsuccessfullywiththesethreekindsofseedingsludges.TheperformanceofthebioreactorinoculatedwithSASwasbetterthanthatinoculatedwithAMS,butworsethanthatinoculatedwithFAS.Afterthestart-up,theVSS/SSratio,granulardiameter,settlingvelocityandSAAofthesethreeseedingsludgesweresuperiortothoseoftheinitialstate.Thecolorofthreeseedingsludgestendedtobebrightred.Besides,thecellsuspensionshowedthecharacteristicabsorptionpeaksbetween400nmand420nm.Thechangesoncolorandtheabsorptionspectrumofthesludgeswerecorrelatedwiththechangesofnumbersandtheactivityofmicroorganismsduringthestart-up.Thecolorandabsorptionspectrumcouldbeusedasanindicatorinthestart-upofAnammoxreactors.Keywords:Anammox;seedingsludges;start-up;sludgecultivation　　2010-07-22,2010-09-20。:。:(1987—),,。:(2009AA06Z311);(2008BADC4B05)。　　[anaerobicammoniumoxidation,Anammox,(1)]　　Receiveddate:2010-07-22.Correspondingauthor:Prof.ZHENGPing,[e-mail]　　[1-3],,9.50kgN·m-3·d-1[4],-(0.23～0.5kgN·m-3·d-1)[5];0.75·(kgN)-1,2～5·(kgN)-1[6]。,Anammox。NH+4+1.32NO-2+0.066HCO-3+0.13H+N2+0.26NO-3+0.066CH2O0.5N0.15+2.03H2O(1),Anammox[0.11gVSS·(gNH+4-N)-1][2],(11d),Anammox[4,7-8],Anammox3.5。Anammox。,Anammox,,Anammox。(anaerobicmethanogenicsludge,AMS)、Anammox(freshAnammoxsludge,FAS)Anam-mox(storedAnammoxsludge,SAS),(ex-pandedgranularsludgebed,EGSB),,,,Anammox。1　1.1　,(g·L-1):KH2PO40.01,CaCl2·2H2O0.0056,MgSO4·7H2O0.3,KHCO31.25;ⅠⅡ1.25ml·L-1。(NH4)2SO4NaNO2,。Ⅰ(g·L-1):ED-TA5,FeSO45。Ⅱ(g·L-1):EDTA15,H3BO40.014,MnCl2·4H2O0.99,CuSO4·5H2O0.25,ZnSO4·7H2O0.43,NiCl2·6H2O0.19,NaSeO4·10H2O0.21,NaMoO4·2H2O0.22。1.2　Anammox1。EGSB,,8.0L,4.0L,,。Anammox-EGSB,(),AnammoxN2。,N2,,。pH6.8～7.0,(35±1)℃。1　Anammox-EGSBFig.1　SchematicdiagramofAnammox-EGSBsystem1—influenttank;2—peristalticpump;3—waterdistributorzone;4—reactionzone;5—sampleports;6—three-phaseseparator;7—settlingzone;8—NaOHsolutiontank;9—gasoutlet;10—effluenttank　1.3　(AMS)、Anammox(FAS)Anammox(SAS),3Anammox-EGSB(R1、R2R3)。ASMIC;FASAnammox;SAS,Anammox3,。33L。1.4　:-[9];:N-(1-)-[9];:[9];(SS)(VSS):[9];:[10]。:LeciaDM2LB·1396·　　　　　62　1　Anammox-EGSBTable1　PerformancesofAnammox-EGSBreactorsstartedupwiththreeseedingsludgesReactorTimeforachievingto0.5kg·m-3·d-1/dTimeforachievingto1.0kg·m-3·d-1/dMaxvolumetricnitrogenloadingrate/kg·m-3·d-1Maxvolumetricnitrogenremovalrate/kg·m-3·d-1R183931.581.39R2007.967.63R3074.413.19(QwinV3)。:-[11]。0.5g,25ml,5ml,UV-2401PC350～800nm,。Anammox(specificAnammoxactivity,SAA):120ml(5g),100ml,5mmol·L-16mmol·L-1,95%Ar5%CO220min,3,。,,ZHWY-2102C,30℃,140r·min-1,,。,SAA[12]。2　　2.1　3AMS、FASSASR1、R2R3,Anammox(50%),。R1、R2R31。3Anammox-EGSB;,3:FASSASAMS。　2.2　32.2.1　污泥颜色和外观的变化　2,,R1,R2,R3();3。2.2.2　污泥吸收光谱的变化　3,,R1407nm,529nm,;R2407nm2　3Fig.2　Apparentcharacteristicsofthreeseedingsludgesbeforeandafterstart-up(a),(c),(e)sludgesinR1,R2andR3beforestart-up;(b),(d),(f)sludgesinR1,R2andR3afterstart-up　,524nm(529nm),,;R3407nm(407nm),529nm,。2.2.3　污泥粒径及其分布的变化　4,,R1(1.69±0.81)mm(1.76±0.91)mm,,,;R2·1397·　5　　:Anammox-EGSB·1398·　　　　　62　5　Fig.5　Changesofsettingvelocitydistributionsofthreeseedingsludgesbeforeandafterstart-up(a),(c),(e)beforestart-upofR1,R2andR3;(b),(d),(f)afterstart-upofR1,R2andR3　(1.39±0.90)mm(1.37±0.86)mm,;R3(1.37±0.80)mm(1.42±0.80)mm,,,。2.2.4　污泥沉降速度及其分布的变化　5,,R1(124.84±48.98)m·h-1(99.30±44.72)m·h-1,125～525m·h-128.07%1.70%;R2(46.39±15.67)m·h-1(48.03±15.65)m·h-1,35～75m·h-182.41%85.05%。R3(47.67±22.28)m·h-1(51.11±16.13)m·h-1,。2.2.5　污泥VSS/SS和SAA的变化　2,,R1R3VSS/SS2　R1、R2R3VSS/SSSAATable2　ChangesofVSS/SSvaluesandSAAofthreeseedingsludgesbeforeandafterstart-upofR1,R2andR3ReactorVSS/SSBeforestart-upAfterstart-upSAA/kgNH+4-N·(kgVSS)-1·d-1Beforestart-upAfterstart-upR10.68±0.010.89±0.010.00±0.000.32±0.04R20.89±0.000.89±0.001.43±0.031.44±0.03R30.79±0.020.91±0.000.05±0.000.92±0.00,R2VSS/SS;R1R3SAA,R2SAA;R1SAA[0.1～0.5kgN·(kgVSS)-1·d-1][13],R2、R3SAA。3　　3.1　Anammox,Anammox·1399·　5　　:Anammox-EGSB,Anammox。,Anammox;,Anammox。,3:R2R3R1。　Anammox400～420nm,c,Anammox。,R1400～420nm,R2400～420nm,R3400～420nm;,3520～550nm。[14-15],756c,315、415、520550nm;c,415nm。(hydroxylamineoxi-doreductase,HAO)(hydrazineox-idase,HZO)Anammox,(3)。Schalk[16-18]AnammoxHAO,420nm524nm,。,,Anammox3　AnammoxHAOHZO[18]Table3　CharacteristicsofHAOandHZOofAnammoxbacteriaFeatureB.anammoxidansHAOStrainKSU-1HZOHAOmolecularmass×10-3183±12130±10118±10subnit×10-3586253compositionα3α2α2h