不同类型人工湿地微生物群落的研究进展周元清

*121＊＊211112000622653100、。、、。DGGEFISH、、、、-、。。Q93A1000－489020116－1251－07Areviewonsoilmicrobialcommunityindifferenttypeconstructedwetlands．ZHOUYuan-qing12LIXiu-zhen1＊＊LIShu-ying2TANGYing-ying1XINZai-jun1JIAYue11StateKeyLaboratoryofEstuarine＆CoastalResearchEastChinaNormalUniversityShanghai200062China2CollegeofEnvironmentalResourcesofYuxiNormalUniversityYuxi653100YunnanChina．ChineseJournalofEcology20113061251－1257．AbstractAsanindispensablepartofconstructedwetlandsoilmicrobesplayanimportantroleinthematerialtransformationandenergyflowinthewetlandecosystem．Thispaperreviewedtheresearchprogressinthedetectionmethodsofsoilmicrobialcommunityitsstructureandcomposi-tionandrelationshipsofsoilmicrobialregulationeffectwithenvironmentalparametersindifferenttypeconstructedwetlands．VariouskindsofnovelmolecularbiologicalmethodshaveprovedtobeusefultoolsforexploringsoilmicrobialdiversityinconstructedwetlandamongwhichdenaturinggradientgelelectrophoresisDGGEandfluorescenceinsituhybridizationFISHwerethemostfrequentlyappliedmethods．Theregulationeffectofsoilmicrobialcommunityinconstructedwet-landmainlydependedonwetlandhydraulicconditionwastewaterpropertyconstituentcomposi-tionandpollutantscharacteristicsandavailabilityfiltermaterialorsoiltypeplantandvari-ousenvironmentalfactors．Thespecies-specificcompositionofsoilmicrobialcommunityindiffer-enttypeconstructedwetlandswasdominatedbyphylumProteobacteriafollowedbythemembersofphylumsProteobacteriaCytophaga－FlavobacteriumActinobacteriaandFirmicutes．Howtofurtherimprovetheresearchesonthediversityofsoilmicrobesinvolvinginnitrogencycleandtoincreasetheremovalefficiencyofnitrogenfromwastewaterwouldbeoneofthekeyissuesforfur-therdevelopmentofconstructedwetlandtechnology．Keywordsmicrobialcommunitymicrobialactivitymicrobialbiomassconstructedwetland．*20100470768、2010RCDW082008KYYW072008ZC161M。＊＊[e-mail]2010-11-222011-02-28ChineseJournalofEcology20113061251－1257DOI:10.13292/j.1000-4890.2011.0222、Matamorosetal．2007Schroderetal．2007。surfaceflowconstructedwetlandsSFCW、horizontalsubsurfaceflowconstructedwetlandsHSS-FCWverticallysubsurfaceflowconstructedwetlandsVSSFCW。SFCW0.4mKadlec＆Knight1996Scholz＆Lee2005。HSSFCWVSSFCW。Vymazal20052008、3。Liikanenetal．2004Manderetal．2005。、3Sundbergetal．2007b。、Nurketal．2005Truuetal．2005Nicomratetal．2006Sundbergetal．2007aTaoetal．2007bTietzetal．2007。、、。、、。1。。。Doran＆Zeiss2000。Dilly2004、、、。、、、、、、、、Schloteretal．2003Bendingetal．2004Truuetal．2008。Kangetal．1998Nguyen2000Shackleetal．2000Nurketal．2005Tietzetal．2007。Tietz2007SIR、ATP。。ReddyDangelo1997。。。、、pH、、。SOD。2521306、、Reddy＆Dangelo1997Liangetal．2003。themost-probable-numberMPNMPN。1%～10%。TorsvikOvreas2002。。rRNA2001。16SrRNA。SSCP、RFLP、DNARAPD、AFLP、DGGETGGEFISHDGGEFISH。DGGEDNATMDNADNADNA。DNA。。。Muzyersetal．1993。Truu2005DGGEHSSFCW。HSSFCW。FISHDNADNA。、2007。Flood1999Shipin2005FISHSFCWVSSFCWα-β-γ-18%～41%。HSSFCW。、、、。2HSSFCW、VSSFCWSFC-Ws。Tietz2007VSSFCW0～10cm、10～20cm20cm。Sleytr200710～20cm。Nguyen2000Nurk2005HSSF-CW。Kern2003。C/N2Nurketal．2005。3521Nicomrat20062008DGGERFLP。Ibekwe20032Acidithiobacillusfer-rooxidansAcidithiobacillusthiooxi-dans。DGGEHSSFCWBacillus、Clostridium、Mycoplasma、EubacteriumPro-teobacteria。Ahn200716SrRNALH-PCR。α-40%～60%ActinobacteriaFirmicutes。DNAWalshetal．2002Lloydetal．2004。Ibekwe20073136operationaltaxonomicunitsOTUs。Walsh200237OTUsIbekwe20072。YuMohn2001RFLPPCR16SrRNA。DO。56OTUsCytophagales。CriadoBecares2005SFCWHSSFCWα-1.9%Shipin200510.5%-Cytophaga-Flavobacterium。Baptista2008FISHDGGEHSSFCW、。。Ahn2007。Ibekwe2007SFCW50%100%。2004BOD5。、-、Yu＆Mohn2001Shipinetal．2005Ahnetal．2007。31994DuncanGroffman1994。2005。ScholzLee2005、、。2006。。Galeetal．1993Kern2003。Ko-zub＆Liehr1999Shackleetal．2000Taoetal．2007a。2008。4521306Kozub＆Liehr1999Sirivedhin＆Gray2006Paredesetal．2007。COD。Tanner2002CODHSSFCW。。Zhou2005VSSFCW、β-。NH4+-NParedesetal．2007Sirivedhin＆Gray2006。Reddy＆Dangelo1997Tanneretal．2002Paredesetal．2007。Braeckevelt2007。Sund-berg2007b。Baptista2003。pH。pH7.0～8.0Paredesetal．2007。20103pH。pH7.0Taoetal．2007b。。5℃4℃Sirivedhin＆Gray2006。Manderetal．2005。＞15℃25℃Paredesetal．2007。Kern2003HSSFCWCO2。、、。4。HSSFCW-Duseketal．2008。。。。DGGEFISHGilbrideetal．2006Nakatsu2007Nockeretal．2007。。。、、。、。Konnekeetal．2005。。．2005．．18644－49．．2001．rRNA5521．21137－43．．2004．．164312－31．．2008．．30675－77．．2006．．262225－229．．2008．．2762401－2406．．2010．．342256－261．．2007．．26457－60．AhnCGillevetPMSikaroodiM．2007．Molecularcharacter-izationofmicrobialcommunitiesintreatmentmicrocosmwetlandsasinfluencedbymacrophytesandphosphorusloading．EcologicalIndicators7852－863．BaptistaJDDavenportRJDonnellyTetal．2008．Themi-crobialdiversityoflaboratory-scalewetlandsappearstoberandomlyassembled．WaterResearch423182－3190．BaptistaJDCDonnellyTRayneDetal．2003．Microbialmechanismsofcarbonremovalinsubsurfaceflowwetlands．WaterScienceandTechnology48127－134．BendingGDTurnerMKRaynsFetal．2004．Microbialandbiochemicalsoilqualityindicatorsandtheirpotentialfordifferentiatingareasundercontrastingagriculturalmanage-mentregimes．SoilBiology＆Biochemistry361785－1792．BraeckeveltMRokadiaHImfeldGetal．2007．Assessmentofinsitubiodegra