不同磷浓度和NP对铜绿微囊藻生长及水环境因子的影响罗东

38《》2[e-mail]。EnvironmentalScience&Technology38720157Vol.38No.7Jul.2015Microcystisaeruginosa7100550.02、0.05、0.1、0.2、0.4、0.5、12mg/L8N/P1∶1、2∶1、4∶1、8∶1、16∶140∶16DO、pH。0.02~0.05mg/L0.1mg/L0.5mg/L0.1mg/LN/PN/P8∶1、16∶140∶18∶116∶1N/P。DO、pHDO、pHDO、pH。N/PDOpHX171.4Adoi10.3969/j.issn.1003-6504.2015.07.0021003-6504(2015)07-0006-04EffectsofDifferentPhosphorusConcentrationsandRatiosofN/PontheGrowthofMicrocystisaeruginosaandtheWaterEnvironmentalFactorsLUODongCHENRongCHENGQingWANGXiaochangYUANHonglinCHENGZheSchoolofEnvironmentalandMunicipalEngineeringXi'anUniversityofArchitectureandTechnologyXi'an710055ChinaAbstractTakingMicrocystisaeruginosaastheexperimentalalgae8differentconcentrationsoftotalphosphorusindifferentreactorsweresetincluding0.02mg/L0.05mg/L0.1mg/L0.2mg/L0.4mg/L0.5mg/L1mg/Land2mg/Lrespectively.Foreachoftheconcentration6differentratiosofN/Pweresetsuchas1∶12∶14∶18∶116∶1and40∶1respectivelytotestthegrowthofalgaecontinuouslyandalsomeasureDOandpHinthereactorsduringthewholeexperimentalperiod.ResultsshowedthattheeffectofdifferenttotalphosphorusconcentrationsonMicrocystisaeruginosagrowthwassignificant.Whentheconcentrationofphosphoruswasintherangeof0.02~0.05mg/Lthealgaegrowthwasslowwhentheconcentrationwasmorethan0.1mg/Lthealgaeobviouslyspeedupthegrowthhoweverwhentheconcentrationofphosphorusexceeded0.5mg/Ltherewerenosignificantdifferencesamongtheconcentrationsoftotalphosphorus.TheeffectsofdifferentratiosofN/Ponthegrowthofalgaewerenotobserveduntiltheconcentrationoftotalphosphoruswasmorethan0.1mg/L.ThegrowthrateofalgaewasinfluencedstronglywhenN/Pwere8∶116∶140∶1andtheoptimalratiosofN/Pforthegrowthofalgaewere8∶1and16∶1.AtthesametimethechangeofthedensityofMicrocystisaeruginosacellshadalsoledtosignificantchangesofDOandpHvaluesintherapidgrowthstageofthealgaethevaluesofDOandpHwererisingwhileinthesteadygrowthstagethevaluesofDOandpHweredropping.KeywordsconcentrationoftotalphosphorusN/PMicrocystisaeruginosaDOpH[1]。[2]。7[3]。、[4-5]。N/P[6-7]N/PTP0.02mg/L2.00mg/L8N/P1∶140∶16DOpH。。11.1。3500r/min10min15mg/LNaHCO3215mg/LNaHCO3。1.2500mLN/PM/mL150mLM×V1150=1.0×105V11×105/mL。KNO3K2HPO4[8-9]KNO3K2HPO4、0.02、0.05、0.10、0.20、0.40、0.50、1.00、2.00mg/L6N/P1∶1、2∶1、4∶1、8∶1、16∶1、40∶1。2000lx12h∶12h70r/min25±0.5℃。。8d48h1。1.320μLCellometer。DO、pHWTWMulti3410。1.4233。22.1N/P8∶1。16∶1[10-11]N/P7.2∶1[12]N/P8∶1N/P。1、、[13-14]4。P=0.02mg/LP=0.05mg/L0.10mg/L≤P≤0.50mg/LP≥1.00mg/L。2.2N/P26N/P。2N∶P≤8∶1N/PN∶P≥8∶1N/P。、N/P0.1mg/LN/PN/P8∶1、16∶140∶18∶116∶1N/P。382.3pHDO34TP=0.4mg/L、N/P=8∶1pHDO3~4pH8.0~9.1DOpHDO8.1~9.0。0~4dDOpH4~6dDOpH6~8dDOpH。33.1N/PN/PN/PN/P。20.05~0.1mg/L0.5~1mg/L。。RedfieldC∶N∶P=106∶16∶1N/P=16N/PN/PN/P。3.2DO、pHDO、pHN/PDO、pH。CO2DO、pHCO2DO、pH。2。pHDO。41。0.02~0.05mg/L0.1mg/L0.5mg/L。20.05~0.1mg/L0.5~1mg/L。2N/P。0.1mg/LN/PN/P8∶1、16∶140∶18∶116∶1N/P。73N/P。[][1].BIOSTYR[J].200520442-45.LuHongyuLyuBingnanWangQingetal.ExperimentalstudyonthetreatmentofBIOSTYRonlandscapewater[J].JournalofShandongUniversityofArchitectureandEngi－neering200520442-45.inChinese[2].[J].201028693-96.ChenYoulanYangWenjin.Watertreatmentprocessofeu－trophiclace[J].EnvironmentalProtectionEngineering201028693-96.inChinese[3]ShangGPShangJC.Spatialandtemporalvariationsofeu－trophicationinWesternChaohuLakeChina[J].Environ－mentalMonitoringAssessment200713012399-109.[4]StevenTRJanS.Effectsoflightdissolvedorganiccarbonandinorganicnutrientsontherelationshipbetweenalgaeandheterotrophicbacteriainstreamperiphyton[J].Hydrobiolo－gia20024891/2/3179-184.[5].[J].200221131-36.LuoDonglian.StudyonthedistributionofdissolvedoxygeninShenhuBayanditsrelationshipwithphytoplanktonandsuspendedmatter[J].MarineScienceBulletin200221131-36.inChinese[6].[J].200730286-89.LuoGuyuanZhuLiangJiTiejunetal.EffectsofdifferentPconcentrationandaerationpatternsongrowthofalgainfreshwaters[J].JournalofChongqingUniversityNaturalScienceEdition200730286-89.inChinese[7].[J].20081751759-1763.FengMaowuWuYunhaiFengShixunetal.EffectofdifferentN/Pratioonalgalgrowth[J].EcologyandEnviron－ment20081751759-1763.inChinese[8]BulgakovNGLevichAP.Thenitrogen/phosphorusratioasafactorregulatingphytoplanktoncommunitystructure[J].ArchivFurHydrobiologie19991463-22.[9]RheeGYGothamIJ.OptimumN/Pratiosandcoexistenceofplanktonicalgae[J].JournalofPhycology198016486-489.[10]ChristopherAKElenaLTanguyDetal.Optimalnitro－gen-to-phosphorusstoichiometryofphytoplankton[J].Na－ture200442913171-174.[11]KlausmeierCALitchmanEDaufresneTetal.Optimalnitrogentophosphorusstoichiometryofphytoplankton[J].Nature2004429171-174.[12]RedfieldAC.Thebiologicalcontrolofchemicalfactorsintheenvironment[J].AmericanScientist1958463205-222.[13].[J].20113969815-9817.LiXiaoshanYangMinLiuFushunetal.Influencesofni－trogen-phosphorusratioonthecompetitivegrowthofalgae[J].JournalofAnhuiAgriSci20113969815-9817.inChinese[14].[J].201142112-16.ZhengJieHuangXianhuaiShangWeietal.ImpactofN/Pratioonalgaandwaterenvironmentfactors[J].IndustrialWaterandWastewater201142112-16.inChinese