不同培养基对富集筛选脱色真菌菌群的效果比较

*贾振杰李慧君杨清香**张昊陈建军(453007):RB5M-3BE,ABD11,5D,AB3A3,50mgLM-3BEA9953%9742%,16,();BD,32,AB,,D:,,,:Q93999:A:0253-2654(2007)04-0629-04EffectsofDifferentMediaonEnrichingandScreeningFungiCulturewiththeAbilitiesofDecolorizingVariousSyntheticDyes*JIAZhen-JieLIHu-iJunYANGQing-Xiang**ZHANGHaoCHENJian-Jun(CollegeofLifeSciences,HenanNormalUniversity,KeyLaboratoryofEnuironmentalPollutionControlTechnologyofHenanProvince,Xinxiang453007)Abstract:Inthispaper3differentmedia(A,foryeastcultivation;B,forlaccaseproducing;D,forwhiterotfungicultivation)werecomparedinenrichingandscreeningdecolorizingfungicultureusingReactiveBlack5(RB5)andReactiveRed(M-3BE)fromthefollowingthreepoints:decolorizationeffects,abilitiesofproducingenzymesanddiversityofmicrobialcommunity.11groupsoffungiwithobviousdecolorizationeffectswereobtainedafterenrichmentfornearonemonth.Amongthem,6groupscamefrommediumD,theothertwo3groupsfrommediumAandB,respectively.However,the3groupsfrommediumAexhibitedthehighestmicrobialdiversityandbestdecolorizationresultswith9953%and9742%colorremovalrateofReactiveRedM-3BEandAcidRed.Fromthem,16strainsoffungiwereisolatedandprimarilyidentifiedasSaprolegniaceae,Eurotiaceae(Monascuswent),ErysiphaceaeandPhysodermataceae.FungigroupsfrommediumBandDexhibitedabitlowercolorremovalrateofvariousdyesandonly3and2isolatesprimarilyclassifiedasSaccharomycetaceaeandEurotiaceae(Penicillium)wereobtainedfromthem.FungiculturesinmediumAandBcouldproduceligninperoxidase,andthoseinmediumDcouldbedetectedhigheractivityoflaccase.Allthefungalculturesexhibitedveryweakactivityofmanganesedependantperoxidase.Keywords:Fungi,Decolorizationofdyes,Medium,Enzyme*(No.NCET-05-0612);(No.20677014)**Tel:[phone],E-mail:yangqx66@163com:2006-09-27,:2006-12-18,,,,[1],,,[2],,629200734(4),[3],,,,,[2~4],,3111A[5]B:45g,15g,1g,NH4Cl05g,100mL:KH2PO42g,MgSO47H2O05g,CaCl201g,KCl05g,1LpH50D[6]1213RB5(m=596nm),M-3BE(m=541nm),(m=516nm),KNR(m=600nm),10mgmL,4141g100mL3,28140rmin,5d~7d1h,50mgLRB5M-3BE5d~7d,100mgL,154,50mgL,28140rmin,[7]=(A0-At)A0100%A0AttOD165000rmin10min,(LiP)Tien&Kirk[8]min1mol[9]240nmmin01ODABTS[10],-,420nm3min,min01OD17,,28,3d,,,221432(M-3BERB5),30,AM-3BE3,A1RA2RA5R,RB5;BB1RB3R,RB5B6B;DD1RD4RD5RRB5D6BD7B1,AM-3BEBD,A45,3,,96h5630200734(4),,48h,,96h20%221150mgLAKNR,,24h,1324hAKNR1(24h%)ABDA1RA2RA5RB1RB3RB6BD1RD4RD5RD6BD7BRB5597687741M-3BE995988803780392688616568124hAKNRA,BKNR1,11A,A1R,A100%KNR,B(B1RB5RB6B)ABD3KNR597%~70%77%~923%361%~629%2311,115,22,A,,;B;D,D1RD4RD6B,,AM-3BE,AM-3BEKNR,A2RA3RD1RD5R,,5968%~6993%BKNR,,213UL~253UL,KNR(1),KNR,,100mg30000U,50,4h,12h,70%[11],,KNR,24h,B6B9232%24,631200734(4)3A16,(),BD5,,A,BDA,23(UmL)M-3BEAKNRLiPLacMnPLiPLacMnPLiPLacMnPAA1R17--210--70--A2R20--18023--1055-A5R--907210-901910-BB1R27--60--253--B3R--10---213--B6B---24060-22727-DD1R----16--2787-D4R---633307--90-D5R223--------D6R----10--3940D7R--30--40--1003:LiP,MnP,lac,-218[1]SafiaM,XamaK,DattaM.DyesandPigments,2007,74(3):723~729[2]KapdanI,KargiF,McMullanG,etal.BioprocessEngineering,2000,22:347~351[3]HeF,HuW,LiY.Chemosphere,2004,57:293~301[4]ChenB,ChenS,LiM,ChangJ,ProcessBiochemistry,2006,41:1574~1581[5]YangQ,YedilerA,YangM,etal,Biochemicalengineeringjournal,2005,24:249~253[6],,.1999,13(2):135~140[7],,.2006,32(3):52~55[8]TienM,KirkTK.MethodsinEnzymology,1988,161:238~249[9],,.,2004,19(2):29~32[10],,.(),2003,2(1):83~90[11],,,.,2004,24(1):48~52[12],,,2005,11(6):763~766论文中有关正斜体的约定:(),,,:,,:BamHIEcoRIMspISau3AI:3,,,,,632200734(4)