不同上升流速IC反应器OCC废水启动过程功能菌群落分布周轩宇

。*2012A032300015。ICOCC*1121221．5106402．510006IC“+OCC”。COD、VLＲ、SMA、F420、DHAFISH。Ｒ12730.76%52．63%2．76Ｒ257Ｒ233．74%1．162．67。ICＲ2ICＲ1。ICOCCF420X793A1671－4571201306－0091－07ICPAQUES2080UASB1、、、。IC4～8m16～25m2IC。IC。ICIC1/2。1。IC、。IC。ICIC3。。FluorescenceinsituhybridizationFISH。ICIC。19《》2013326DOI:10.19696/j.issn1671-4571.2013.06.02311．1ICＲ11/2ICＲ29L1/3。3000mg/LCOD24h。COD5kgCOD/m3·dCOD15kgCOD/m3·d。COD80%。Ｒ12715kgCOD/m3·d4．8h10。Ｒ25715kgCOD/m3·d4.8h18。11．2“+OCC”COD∶N∶P=200∶5∶1。、KHPO4、NH4Cl、CaCl2、NaHCO3COD10%、20%、50%1/10000411/g·L－1FeSO4·7H2O1．12MnCl2·4H2O0．2NiCl2·6H2O0．02NH46Mo7O24·4H2O3．6/g·L－1CaCl2·6H2O0．8ZnCl20．02CuSO4·5H2O0．018H3BO30．02EDTA0．41．3。TSS105．95g/LVSS70．4g/LVSS/TSS0．6611．07mLCH4/hgDHA65.603TFμg/mLF4200．1165μmol/gVSS50．00%±2．33%2．47%±0．19%13．3%±0．42%。9L1/3。1．4CODXJ－Ⅲ5VSSTSS6F42067TTC89。1．5FISH1．5．1FISH、。311。SYNM700SYNM700Syn-trophomonasspp．10AWAWAcetobacteriumsp．E．limosum11MG1200MG1200Methanomicrobia-les121。TAMＲAFITC5'3'HPLC。29PaperScience＆Technology2013Vol．32No．625’to3’SYNM700ACTGGTＲTTCCTC-CTGATTTCTASyntrophomonasspp．TAMＲAAWGGCTATTCCTTTC-CATAGGGAcetobacteriumsp．E．limosumFITCMG1200CGGATAAT-TCGGGGCATGCTGMethanomicrobialesTAMＲA1．5．2FISH13－20℃。K141546℃2h50%、80%、95%、100%1∶85ng/mL1646℃Thermo-BriteIris2h。。DAPI17。LeicaTCSSP5Leica18。ImageProPlus1920。1．5．3。、ImageProPlus119DAPI。%=×100%122．12。Ｒ19kgCOD/m3·dCOD73．2%COD80%27Ｒ115kgCOD/m3·d4．8hCOD92．7%。Ｒ1Ｒ2COD43．5%CODCOD80%。Ｒ2Ｒ1。Ｒ1Ｒ2Ｒ2。Ｒ2Ｒ1COD80%。57Ｒ215kgCOD/m3·d4．8hCOD92．3%。Ｒ2。Ｒ2。ICＲ2ICＲ1。2COD2．2、F420。5kgCOD/m3·d、10kgCOD/m3·d、15kgCOD/m3·dVSSF420。3、439《》2013326F420、Ｒ1Ｒ2。11.07mLCH4/h·gF4200.1165μmol/gVSS13.3%±0.42%。Ｒ126.66mLCH4/h·gF4200.2602μmol/gVSS50.00%±1.24%Ｒ222.97mLCH4/h·gF4200.2328μmol/gVSS42.86%±1.12%。。5kgCOD/m3·dＲ2VFAＲ213.30%±0.42%12.12%±0.52%。。3、4、F420。Ｒ2Ｒ1Ｒ2Ｒ1Ｒ2Ｒ1。34F4202．321。、lhμgTF。532．8TFμg/mL·h5kg-COD/m3·dＲ172．1TFμg/mL·hＲ254．6TFμg/mL·hＲ197．1TFμg/mL·hＲ2102．8TFμg/mL·h。。Ｒ2。Ｒ2Ｒ1Ｒ1Ｒ2Ｒ1。Ｒ2Ｒ2Ｒ1Ｒ2Ｒ1Ｒ2Ｒ1。Ｒ2Ｒ2。。49PaperScience＆Technology2013Vol．32No．6、。52．4IC7。2Ｒ130.76%52．63%2．76Ｒ233．74%1．162．67Ｒ2Ｒ12．28%Ｒ141．38%Ｒ12．28%。622。Ｒ2。Ｒ2、Ｒ2Ｒ1。。350．00%±2．33%2．47%±0．19%13．3%±0．42%Ｒ165．38%±2．09%3．77%±0．17%50．00%±1．24%Ｒ266．87%±1．71%5．33%±0．07%48．86%±1．12%59《》2013326633．1ICＲ127d15kgCOD/m3·d4．8hCOD92．7%。ICＲ257d15kgCOD/m3·d4.8hCOD92.3%。Ｒ130.76%52.63%2.76Ｒ233．74%1．162．67。3．2、。。3．3Ｒ2。7FISH69PaperScience＆Technology2013Vol．32No．6ICＲ2ICＲ1。1．J．2010503400－402．2．M．2005．108．3．EGSBJ．2006221562－66．4．．ICJ．201127333－36．5．Umax-CH4J．19851431－35．6．M．1990．7．F420J．19908211－15．8．MBＲJ．2006231231－33．9．TTC－J．1995311－13．10HertoDATsukasaIKazumiYetal．PhylogeneticandFunction-alDiversityofPropionate-oxidizingBacteriainanAnaerobicDi-gestersludgeJ．ApplMicrobiolBiotechnol200775673－683．11KüselKPinkartHCDrakeHLetal．AcetogenicandSulfate-re-ducing-bacteriaInhabitingtheＲhizoplaneandDeepCortexCellsoftheSeaGrassHalodulewrightiiJ．ApplEnvironMicrobiol1999655117－5123．12ＲaskinLStrinmleyJＲittmannBEetal．GroupSpecific16SＲNAhybridizationProbestoDescribeNaturalCommunitiesofmethanogensJ．ApplEnvironMicrobiol1994601232－1240．13NuriaFEmilianoEDＲicardoAetal．AnalysisofMicrobialCommunityduringBiofilmDevelopmentinanAnaerobicWastewatertreatmentreactorJ．MicrobiolEcol200861121－132．14．FISHJ．2008404546－549．15．J．2002314362－363．16．J．20103171554－1560．17．J．20113045－8．18．J．201218149－50．19WallnerGAmannＲ．BeikerW．．OptimizingFluorescentinSituHybridizationwithrＲNA－targetedOligonucleotideProbesforFlowCytometricIdentificationofMicroorganismsJ．Cytometry199314136－14320．FISHJ．20074761038－1043．21．D．2006．17－18．22．J．2006262183－187．FunctionalBacteriaCommunityDistributioninDifferentＲiseVelocityICＲeactorStart-upProcedurebyOCCWastewaterZhouXuan-yu1MaYong-wen12WanJin-quan12WangYan21．StateKeyLaboratoryofPulpandPaperEngineeringSouthChinaUniversityofTechnologyGuangzhou510006GuangdongChina2．CollegeofEnvironmentandEnergySouthChinaUniversityofTechnologyGuangzhou510006GuangdongChinaAbstractThetraditionalICreactorＲ1andthenewtwopointreturnflowICreactorＲ2whoserisevelocitywasabout1/2ofthetraditionalICreactor’sinthesameotherconditionswasstartedupundernormaltemperature．Bythewayofenteringwaterof“composedwaterbyglucose+OCCwastewater”theratioofOCCwastewatertocomposedwatercouldbegraduallyincreased．CODremovalratevolumetricloadingrateVLＲspecificmetha-nogenicactivitySMAcoenzymeF420dehydrogenaseactivityDHAinthetwoICreactorsinthestart-upproce-durewasresearchedandfluorescenceinsituhybridizationFISHwasusedtobeacharacterizationoffunctionalbacteriacommunitydistributioninthetworeactors．Theresultsshowasthestart-upwassuccesscomparedwiththeseedsludgetheabundanceofthebutyrate-oxidizingacetogenicbacteriaSyntrophomonasspp．increasesby30．76%thehomoacetogenicbacteriaAcetobacteriumspeciesE．limosumincreasesby52．63%andmethanomi-crobiummethanomicrobialesincreasesby276%inＲ1．Andtheabundanceofthebutyrate－oxidizingacetogenicbacteriaSyntrophomonasspp．increasesby33．74%thehomoacetogenicbacteriaAcetobacteriumspeciesE．limo-sumincreasesby116%andmethanomicrobiumMethanomicrobialesincreasesby267%inＲ2．AlthoughＲ2tookalongtimetobestartedupreducedthedifficultyofmachiningandmaintenancegreatlybyreducingtheeffectiveheight．AndＲ2