不同物质对亚硝酸盐积累的影响冉竞龙

29720097　　　　　　ActaScientiaeCircumstantiaeVol.29,No.7Jul.,2009:(No.50678071);(No.06105409)SupportedbytheNationalNaturalScienceFoundationofChina(No.50678071)andtheNaturalScienceFoundationofGuangdongprovince(No.06105409):(1984—),,E-mail:[e-mail];＊(),E-mail:[e-mail]:RANJinglong(1984—),male,E-mail:[e-mail];＊Correspondingauthor,E-mail:[e-mail],,.2009.[J].,29(7):1405-1410RanJL,HuYY,YanJ.2009.TheeffectsoforganiccarbonandsaltsonnitriteaccumulationinanANNAMOXreactor[J].ActaScientiaeCircumstantiae,29(7):1405-1410冉竞龙,胡勇有＊,阎佳华南理工大学环境科学与工程学院,广州510006:2008-10-27　　　:2009-05-07:-.SBR,,.,COD200mg·L-1(0C/N≤0.34),,78%;,COD,COD,98.4%.、,0.5mol·L-1、0.4mol·L-10.4mol·L-1,,;:C/N.:;;;;:0253-2468(2009)07-1405-06　　　:X703　　　:ATheeffectsoforganiccarbonandsaltsonnitriteaccumulationinanANNAMOXreactorRANJinglong,HUYongyou＊,YANJiaCollegeofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou510006Received27October2008;　　　accepted7May2009Abstract:NitriteaccumulationisregardedasthekeytotheNitrification/Anammoxprocess.TheeffectofdifferentorganiccarbonandsaltsinthefeedingofSBRreactorswasstudiedaftersteadynitrificationwasachievedinthereactors.TheresultsshowedthatwhentheinfluentCODwasnotmorethan200mg·L-1(0C/N≤0.34),thenitriteaccumulationratewaspromotedupto78%.However,differentkindsoforganiccarbonhadmuchmoreobviousimpactsontheCODremovalratethannitrification.Whenglucosewasusedasorganiccarbon,theCODremovalratewas98.4%whichwasbetterthananyothertestedcarbonsource.Thenitriteaccumulationdecreasedrapidlywhentheconcentrationofchlorine,sulfateorphosphateincreased,andtheactivityofammoniaoxidizingbacteriawasrestrained.Nitriteaccumulationwascompletelyinhibitedwhentheconcentrationofchlorine,sulfateorphosphatereached0.5mol·L-1、0.4mol·L-1and0.4mol·L-1,respectively.TheorderofinfluenceoftheC/Nratioandsaltsonnitriteaccumulationwas:sulfatephosphatechlorineC/N.Keywords:nitrite;organiccarbon;chlorine;sulfate;phosphate1　(Introduction)-,1∶1.3.、pH、(FA)、(DO)、(HRT).pH7.5～8.5(HellingaC,etal.,1998;,2000;,1999)、30～35℃(Mulder,1997)、SRT1d,DO0.5～1mg·L-1(Hanaki,etal.,1990;Laanbroek,1993;,2000;Alleman,1984;Bernat,2001;Wiesmann,1994)、,.DOI:10.13671/j.hjkxxb.2009.07.013　　　　　　　　　　29,,、、.A.Mosquera-Corral(Mosquera-Corraletal.,2005)CSTRTOC/N,TOC/N0.2,,TOC/N0.3,,10%;NaCl、KCl、Na2SO4,Cl-0.5mol·L-1,.Nowak(Nowaketal.,1996)(0.2～0.03mg·L-1).Arquiaga(Arquiagaetal.,1993),、、、,.,,..SBR,SBRCSTR,C/N≤0.34,SBRCOD90%,0.17kg·m-3·d-1(,2008).,C/N=0.34,(、)(、),.2　(Materialsandmethods)2.1　实验装置及运行参数0.5LSBR,(30±1)℃.SBR,MLSS2500mg·L-1,1～2mg·L-1,80%,HRT1d.1.1　(1.,2.,3.,4.,5.SBR)Fig.1　Schematicdiagramoftheexperimentalequipment2.2　进水水质,()600mg·L-1,COD200mg·L-1,、、、、、;、、.1.1　Table1　CompositionofthefeedingsolutionCaCl2/(g·L-1)KH2PO4/(g·L-1)MgSO4/(g·L-1)FeSO4·7H2O/(g·L-1)NaHCO3/(g·L-1)EDTA/(g·L-1)1)/(mg·L-1)pH0.300.070.020.0092.160.0061.257.55±0.50　　1)(Strousetal.,1998).2.3　分析项目与测定方法:;:N—(1-)—;:;COD:;pH:EcoscanpH5/6;DO:YSI550A;MLVSS、MLSS:((4),2002;(),1985).3　(Results)3.1　不同碳源对亚硝酸盐积累的影响　　2,1,2～8、、、、、.153.7%;2～8,,79.3%,,76.4%;14067:2COD,98.4%,COD66.0%～84.6%.2　(1.;2.;3.;4.;5.;6.;7.;8.)Fig.2　Theeffectofdifferentorganiccarbonsourcesonnitriteaccumulation　2.2　不同离子对亚硝酸盐积累的影响2.2.1　氯离子对亚硝酸盐积累的影响　2.1,,().(3),.,Cl-0.043mol·L-1,,79.0%,473.4mg·L-1,136.5mg·L-1.Cl-0.5mol·L-1,,.3　Cl-Fig.3　Theeffectofchlorineonnitriteaccumulation4,CODCl-.,COD99.5%.Cl-0.5mol·L-1,COD22.6%.Cl-0.5mol·L-1,COD,.4　Cl-CODFig.4　TheeffectofchlorineontheCODremovalrate2.2.2　SO2-4对亚硝酸盐积累的影响　5,、CODSO2-4,.,COD,79.0%99.6%.SO2-40.1mol·L-1,,42.3%,,COD78.1%.SO2-40.4mol·L-1,,,COD54.4%.5　SO2-4CODFig.5　TheeffectofsulfateonnitriteaccumulationandCODremovalrate　1407　　　　　　　　　　292.2.3　PO3-4对亚硝酸盐积累的影响　6,、CODPO3-4,.PO3-40.02mol·L-1,COD,76.8%98.2%.PO3-40.02mol·L-1,COD6　PO3-4CODFig.6　TheeffectofphosphateonnitriteaccumulationandCODremovalrate　.PO3-40.4mol·L-1,,,COD70.9%.2.3　正交实验2.2,Cl-、SO2-4、PO3-4C/N43(2),,3.2　Table2　FactorsandlevelsintheorthogonalexperimentCl-/(mol·L-1)SO2-4/(mol·L-1)PO3-4/(mol·L-1)C/N10.04200020.1500.150.150.3430.3000.300.300.673,SO2-4,Cl-PO3-4,C/N.3　Table3　ResultsoforthogonalexperimentsCl-/(mol·L-1)SO2-4/(mol·L-1)PO3-4/(mol·L-1)C/NNO-2-N/(mg·L-1)10.042000332.520.0420.150.150.3441.630.0420.30.30.679.340.1500.150.6773.850.150.150.306.660.150.300.34070.300.30.347.180.30.1500.6714.490.30.30.1500Ⅰ127.8137.8115.6113.0Ⅱ26.820.938.516.2Ⅲ7.23.17.932.5　　:Ⅰ、Ⅱ、Ⅲ1、2、3,3.4　(Discussion)4.1　不同碳源对亚硝化的影响,,,,,;,,,,,,(Philipsetal.,2002).,(2～8)78.0%,(153.66%)24.34%,14087:,SteinmullerBock、(SteinmullerandBock,1976;,2008).Mosquera-Corral(Mosquera-Corraletal.,2005)CSTR,,150mgVSS·L-1,C/N00.2,43%60%;C/N0.3,.C/N0.34,1～875.0%,,:SBRCSTR;SBR(2500mg·L-1,MLVSS/MLSS70%).,2～8,COD200mg·L-1,,COD.(2～4)COD(5～8)(COD86.6%、68.4%),COD.C/N0.34,,,,.4.2　不同离子对亚硝化的影响,,,,COD.(Cl-、SO2-4PO3-4)0.5mol·L-1、0.4mol·L-10.4mol·L-1,,Mosquera-Corral(Mosquera-Corraletal.,2005).COD22.6%、54.4%70.9%,,.0.5mol·L-1,CODCOD,,,,,,,COD.SO2-4PO3-40.4mol·L-1,COD,.,.,,0.3mol·L-1,,,,(,2004).5,0.02mol·L-1(0.61mg·L-1),,Nowak(Nowaketal.,1996),0.03mg·L