不同植物人工湿地净化效果及基质微生物状况差异分析

24920119ResearchofEnvironmentalSciencesVol．24No[e-mail]．*[e-mail]121*211RHEEJaeSeong3IAMCHATURAPATRJanjit31．1000122．4101283．KIST130－650、、、5HRT135dTPNH4+-NCODCr．HRTHRT5d．TPNH4+-NCODCr90.4%61.7%96.4%．．．NH4+-N．X703A1001－6929201109－1035－07PurificationEffectsandDifferencesinSubstrateMicroorganismStatusinConstructedWetlandsVegetatedwithDifferentPlantsFENGXiao-you12SONGYong-hui1ZENGQing-ru2PENGJian-feng1LIUXue-yu1RHEEJaeSeong3IAMCHATURAPATRJanjit31．ChineseResearchAcademyofEnvironmentSciencesBeijing100012China2．CollegeofResourcesandEnviranmentHunanAgricultureUniversityChangsha410128China3．KoreaInstituteofScienceandTechnologySeoul130-650KoreaAbstractInordertostudytheeffectsofwetlandvegetationonpollutantremovalefficienciesandsubstratemicroorganismstatussimulatedsequencingbatchconstructedwetlandswerevegetatedwithfivetypesofplantsAcoruscalamusTyphaorientalisLythrumsalicariaLinnPhragmitesaustralisandSpathiphyllumwallisii．TheremovalefficienciesofTPNH4+-NCODCrandtheamountofsubstratemicroorganismswerestudiedunderthreedifferenthydraulicretentiontimesHRT13and5d．TheresultsshowedthatthepollutantremovalratesincreasedgraduallywiththeextentofHRT．ThehighestTPNH4+-NandCODCrremovalefficiencieswere90.4%61.7%and96.4%obtainedataHRTof5d．IncomparisonwithothersystemstheAcoruscalamussystemhadthehighestpollutantremovalefficienciesunderallHRTs．Nosignificantdifferencesofthemicroorganismabundancewereobservedamongdifferentvegetations．HighcorrelationwasshownbetweensubstratenitritebacteriaandtheNH4+-Nremovalinallsystems．Keywordsconstructedwetlandwetlandplantshydraulicretentiontimesubstratemicroorganisms2070、、24．、、、1．2．3-4．5、、、．6、、、．7．8．9TNTPTNTP．10BOD5TNTP．HUETTA11TNTPTNTP．．、、、、、．HRT．5、、、HRTHRT．11.1130cm50cm35L．．10～15mm13cm2～6mm5cm18cm．10cm．36%9.3L．1Fig．1Schematicdiagramoftheexperimentalequipment1.2、、、511212．21．30cm．2～3cm．HRTHRT135d95d．HRT163019d153d105d10．———．．25～35℃．pHρDOρNH4+-NρTPρCODCrNH4HCO3KH2PO4．pH7～9ρNH4+-N130～160mgLρTP6～8mgLρCODCr580～600mgL。1.312．ρCODCrρTPρNH4+-NρNO3－-NρNO2－-NTρDOYSIDOYSIpHpHWTW．1.48～10cm330g3、、．13．1.5SPSS13.0HRTHRT1010、HRT．22.1NH4+-N2HRT13dNH4+-N30%～40%．HRTNH4+-N．HUANG14NH4+-N．HRT5dNH4+-N13d．①HRTNH4+-N②HRT5dρCODCrCODCr．MAYO15HRTHRT5d8dTN33.4%43.4%．HRTd1—12—33—5.2NH4+-NFig．2ComparisonofNH4+-Nremovalrateinallsystems16．NH4+-NNH4+-NρDO17．ρDO0.5～0.8mgL3NH4+-N．18-20．BRIX2130%．REED19NH4+-N．HRTNH4+-N．2HRT5dNH4+-NρDO．2.2TP4TP．HRT1dTP58.9%46.9%．730124HRTd1—12—33—5.3ρDOFig．3VariationofρDOinallsystemsHRTd1—12—33—5.4TPFig．4ComparisonofTPremovalrateinallsystems．TPKYAMBADDE3．HRT3dTP50%TP．HRT5dTP90.4%68.0%．TP．2217．323．3＞≥＞≥24．HRT、TP．35d．3d．HRT5dP．．HRT5d．2.3CODCrρCODCr600mgLCODCrCODCr．CODCr．HRTCODCr．HRTCODCr．HRT3d1dHRT5d13d．HRTCODCrHRT5dNH4+-N13dρNO3－-NNO3－-NCODCr．25NH4+-NTPCODCr60%85.28%．HRT5dCODCr．HRTd1—12—33—5.5CODCrFig．5ComparisonofCODCrremovalrateinallsystems2.40.05～0.12kg．830190.725kg0.684kg、0.667kg、0.536kg0.443kg．AU6.926.78、5.54、5.574.76．NH4+-NTP．26-27、、．289、、、．MANTOVI29、．2.514、．1P＜0.01．NH4+-N30-31．14Table1Thequantityof4bacteriaineverysystem106g－1103g－1103g－1106g－121.3010.404.9716.6021.904.633.7214.2031.404.863.885.3123.904.363.6732.908.203.713.0231.901.04×104g－1、、．NH4+-NR2=0.8996ρNH4+-N．NH4+-N．4.97×103g－1、．6NH4+-NFig．6ThecorrelationbetweentheremovalratesofNH4+-Nandnitrobacteriaineverysystem4～101～1.2．ρDONH4+-NNO2－-N．NO2－-N－．DO．GREEN32ρDO．ρDOρDO3．NH4+-NρDO．NO3－-NNO3－-NN2N2O6CH2O+4NO3→－6CO2+2N2+6H2O19301243.29×107g－1、、．ρNO3－-N〔0.52±0.34mgL〕〔0.47±0.30mgL〕、〔0.44±0.23mgL〕〔0.44±0.29mgL〕〔0.42±0.27mgL〕．ρNO3－-N．ρDOρDOρNO3－-N．3a．HRTTP．NH4+-N．CODCr．b．HRT．NH4+-NCODCrHRT．TPHRT3d1dHRT5dTPHRT．HRT5d．c．、．．NH4+-N．d．NH4+-NTPCODCr．References1IAMCHATURAPATRJYISWRHEEJS．Nutrientremovalsby21aquaticplantsforverticalfreesurface-flowVFSconstructedwetlandJ．EcologicalEngineering2007295287-293．2．、J．1999334575-578．3KYAMBADDEJKANSIIMEFGUMAELIUSLetal．Acomparativestudyofcyperuspapyrusandmiscanthidiumviolaceum-basedconstructedwetlandsforwastewatertreatmentinatropicalclimateJ．WaterRes2004382475-485．4COLLINSBMCARTHURJVSHARITZRR．PlanteffectsonmicrobialassemblagesandremediationofacidiccoalpilerunoffinmesocosmtreatmentwetlandsJ．EcologicalEngineering2004232107-115．5．J．2009223337-342．6．、J．201032636-40．7．J．200726S1249-251．8．J．2009303713-719．9．TNTPJ．200720375-80．10．J．200518644-49．11HUETTADOMORRISBSGSMITHAetal．NitrogenandphosphorusremovalfromplantnurseryrunoffinvegetatedandunvegetatedsubsurfaceflowwetlandsJ．WaterRes200539143259-3272．12．M．4．2002．13．M．1985．14HUANGJRENEAUJHAGEDORNC．NitrogenremovalinconstructedwetlandsemployedtotreatdomesticwastewaterJ．WaterRes20003492582-2588．15MAYOAWMUTAMBAJ．EffectofHRTonnitrogenremovalinacoupledHRPandunplantedsubsurfaceflowgravelbedconstructedwetlandJ．PhysicsandChemistryoftheEarth200429151253-1257．16CRITESRWDOMBECKGDWASTSONRCetal．RemovalofmetalsandammoniainconstructedwetlandsJ．WaterEnvironRes1997692132-135．17ANTONIOUPHAMILTONJKOOPMANBetal．EffectoftemperatureandpHontheeffectivemaximumspecificgrowthrateofnitrifyingbacteriaJ．WaterRes199024197