常温下厌氧氨氧化污泥的储存及活性恢复黄佳路

3811201711ENVIＲONMENTALSCIENCEVol．38No．11Nov．2017*150090AnAOB．14～30℃15、30、45、60、75100d．15、30、45、60、75100dSAASAA90.9%、64.3%、61.7%、43.2%、25.8%19.3%．SAAＲ20.978．SAASAA103.4%、129.3%、124.8%、111.7%116.9%100dSAASAASAA98.9%．100dSAAN/VSS0.0513g·g·d－19.5dSAA．X703.1A0250-3301201711-4687-09DOI10．13227/j．hjkx．2017041592017-04-152017-05-29ZD2014121991～[e-mail]*[e-mail]ofAnaerobicAmmoniumOxidationANAMMOXSludgeatＲoomTemperatureHUANGJia-luWANGXiao-longGAODa-wen*StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnologyHarbin150090ChinaAbstractSlowgrowthrateslimitthewidespreadapplicationofanaerobicammoniaoxidationANAMMOXreactionswhichpresentsaneedforthestudyoflong-termstorageandrapidreactivationofANAMMOXsludge．Underroomtemperatureconditions14～30℃thisstudyinvestigatedtheeffectsofstoragedurationontheresidualactivityandtheactivityafterreactivationofANAMMOXsludgewithouttheadditionofexternalsubstances．Thechosenstoragedurationswere1530456075and100daysrespectively．TheresultsshowthattheresidualspecificANAMMOXactivitiesSAAsofthestoredANAMMOXsludgewere90.9%64.3%61.7%43.2%25.8%and19.3%oftheinitialactivityactivitybeforesludgestorageafter1530456075and100daysrespectively．ThereforetheresidualSAAdecreaseslinearlywithanincreaseinthestoragedurationofANAMMOXsludgeＲ2was0.978．TheSAAsafterreactivationwithastoragedurationof15304560and75dayswereallhigherthantheinitialSAAandreached103.4%129.3%124.8%111.7%and116.9%oftheinitialSAAbeforeitsstoragerespectively．HowevertheSAAafterreactivationafterstoragefor100dayswasjust98.9%oftheinitialSAA．Inconclusionafteraslongas100daysofstoragedurationunderroomtemperaturetheresidualSAAofANAMMOXsludgedecreasesto0.0513g·g·d－1anditsANAMMOXactivitycanstillberecoveredafter9.5daysofreactivationculture．KeywordsroomtemperatureanaerobicammoniaoxidationANAMMOXsludgestoragestoragedurationreactivationspecificANAMMOXactivitiesANAMMOX．NO－2-NNH+4-NN2NO－31．、、-．AnAOB11～19d2．ANAMMOX423．AnAOB、pH、、、、4～8．．9．38、、．－200℃4104℃511．12．4℃NH42SO4AnAOB13．AnAOB14．．14～30℃15、30、45、60、75100dSAAAnAOB．11.1EGSB3L．NH+4-NΔNH+4-N、NO－2-NΔNO－2-NNO－3-NΔNO－3-N1∶1.1∶0.231∶1.32∶0.261．NH+4-NNO－2-N90%8.21kg·m3·d－1SAAN/VSS0.266g·g·d－1．．1.2NH42SO4NaNO2NH+4-NNO－2-N．KHCO31500mg·L－1、KH2PO425mg·L－1、CaCl2·2H2O140mg·L－1MgSO4·7H2O200mg·L－1、1mL·L－1．EDTA5000mg·L－1FeSO45000mg·L－1ZnSO4·7H2O430mg·L－1CoCl2·6H2O240mg·L－1MnCl2·4H2O990mg·L－1CuSO4·5H2O250mg·L－1NaMoO4·2H2O220mg·L－1NiCl2·6H2O190mg·L－1NaSeO4·10H2O210mg·L－1H3BO414mg·L－1．30min．1.3．30℃14℃1．1Fig．1Indoortemperaturechangesduringsludgestorage15、30、45、60、75100d3SAASAA．70mg·L－1NH+4-N92mg·L－1NO－2-NNH+4-NNO－2-NSAA．33g140r·min－135℃HＲT12h．．．1.4SAASAANH+4-NNO－2-N70mg·L－192mg·L－1．pH7.7～8.1．100mL3g20min．35℃、140r·min－11hNH+4-NNO－2-N．886411SAA．3．1.5VSS、NH+4-N、NO－2-NNO－3-N、、N-1--15．pHWTWMulti3430．a15db30dc45dd60de75df100d3Fig．3ChangesofANAMMOXsludgecolorsduringstorage22.1SAA2.1.1SAA15、30、45、60、75、100dSAA2．SAA0.242、0.171、0.164、0.115、0.0690.051g·g·d－1SAA90.9%、64.3%、61.7%、43.2%、25.8%19.3%．15d15dAnAOB．15～100dＲ20.978．100dSAA19.3%16．100dSAA15d．2SAAFig．2VariationsoftheSAAafterstorageunderdifferentstoragedurations2.1.2．3．．AnAOB1718．986438AnAOB．19c．．13．AnAOBH2S．2.22.2.14．15d4a24hNH+4-NNO－2-N1mg·L－148h99%．15dSAA2d．15d30d4b．72hNH+4-N3.5mg·L－196.1%．NO－2-N3.1mg·L－197.5%108h96%4.5d．45d4c108hNH+4-NNO－2-N79.1%、81.5%4.5d．60d4d60hNH+4-NNO－2-N80%．72hNH+4-NNO－2-N22.8%47.1%．．0.5%2021．144hNH+4-NNO－2-N12.1mg·L－114.9mg·L－1．6d30d45d4.5d6d．60d75d4e144hNH+4-NNO－2-N85%．6d．100d4fSAA0.0513g·g·d－1．84hNH+4-NNO－2-NNH+4-NNO－2-N30%．nNH+4-NnNO－2-N1∶1AnAOB22．228hNH+4-NNO－2-N23.5mg·L－115.0mg·L－167.7%80.8%．9.5d．NH+4-NNO－2-NSAA．SAA．15、30、45、60、75100d9.5dSAA．AnAOB．AnAOB11～19d．．．．．0964114Fig．4PerformanceafterdifferentstoragedurationsofANAMMOXsludgeunderroomtemperature．．Ali23AnAOB3mmol·L－190d35dAnAOBJi244℃60d14d92.9%Xing254℃50d8d．．100d9.5d．．2.2.2SAA15、30、45、60、75、100d196438SAA5．SAA0.275、0.344、0.332、0.297、0.3110.263g·g·d－1SAA103.4%、129.3%、124.8%、111.7%、116.9%98.9%．100dEGSBSAASAA．AnAOB16．a15db30dc45dd60de75df100d6Fig．6ChangesofANAMMOXsludgecolorsduringrestoration5Fig．5ＲeactivationafterdifferentstoragedurationsofANAMMOXsludge2.2.3．6．6a15d2d．．360、75100d6d～6f．AnAOB．6．．2.3ΔNH+4-N、ΔNO－2-NΔNO－3-N1∶1.32∶0.261．7．15d60d2964117Fig．7VariationsofstoichiometricratiosafterdifferentstoragedurationsunderroomtemperatureintherestorationstageofANAMMOXsludgeΔNO－2-N/ΔNH+4-N1.32ΔNO－3-N/ΔNH+4-N0.26．AnAOB、2627．AnAOB．AnAOBNH+4-NNO－2-NNO－3-NNO－2-NNO－3-NΔNO－2-N/ΔNH+4-N1.32ΔNO－3-N/ΔNH+4-N0.2611．ΔNH+4-N、ΔNO－2-N、ΔNO－3-N1∶1.32∶0.26．pH．4SAA．3964387e7f75d100dΔNO－2-N/ΔNH+4-NΔNO－3-N/ΔNH+4-N．AOBNH+4-N28．EGSB．AOB．AOBNH+4-N．AOBAnAOB．31AnAOB100dSAASAA19.3%．SAAＲ20.978．215～100d2～9.5dSAA．100dSAASAASAA．．1StrousMKuenenJGJettenMSM．KeyphysiologyofanaerobicammoniumoxidationJ．AppliedandEnvironmentalMicrobiology19996573248-3250．2．J．2012325843-849．YuYCGaoDWTaoYetal．Start-upofanammoxinasequencingbatchbiofilmreactorSBBＲJ．ChinaEnvironmentalScience2012325843-849．3VanderStarWＲLAbmaWＲBlommersDetal．Startupofreactorsforanoxicammoniumoxidationexperiencesfromthefirstfull