除油菌的筛选及其处理采油废水的研究申泰铭

246Vol.24No.6　　　　　JOURNALOFQINZHOUUNIVERSITY200912　Dec.,2009　　　[]2009-11-11　　[]申泰铭(1982-),男,广西桂林人,钦州学院生物与化学系讲师。除油菌的筛选及其处理采油废水的研究,(,535000)　　[　]　通过对活性污泥的驯化,筛选处理采油废水的高效菌株。筛选得到三个属共5株高效菌株,除油能力在25.06～52.09%之间,COD去除能力在32.39～87.44%之间。混合菌生长迅速,没有延滞期,石油类最终去除率为94.34%,COD最终去除率为92.34%。高效细菌对石油类的去除率并不随着石油类含量的增加而上升。混合菌在石油类浓度为26.84mg/L时具有最高去除率96.64%;在COD浓度为491mg/L时具有最高去除率97.50%。混合菌株对芳香烃的去除能力最强,达到88.91%。对菌株进行紫外线辐照处理,获得3株诱变菌株,诱变菌株的除油能力以及除COD能力均有提高。[]　诱变育种;高盐度采油废水;生物处理[]X172　　[]A　　[]1673-8314(2009)06-0030-05　　,,,。[1]。,[2,3],,;,,,,,,。,[4,5]。,。,,[6,7,8]。,,,,[9,10,11]。,118,5,。,,。,3,COD。1　1.1　。,1:1Table1ThequalityofrawwastewaterCOD350-640mg/L27.4-31.8g/L10.5-21.4mg/LpH7.8-8.2　　。:KH2PO450g、K2HPO450g、Mg2SO420g、CaCl210g、MnSO40.1g、FeCl310g、NH4NO310g、H2O100mL。第6期申泰铭,戴梓茹:除油菌的筛选及其处理采油废水的研究:0.5g、1g、100mL、1mL。:(1)0.5g、1g、100mL、1mL、2g;(2)20g、100mL、1mL;(3)2g、0.1g、100mL、1mL。:100mL、1mL、2g。:100mL、1mL、0.0015、0.0020、0.0025、0.0040、0.0050g。1.2　1.2.1　耐盐除油细菌驯化及筛选,。,,30℃2d,30℃2d。100mL,0.1Mpa,120℃30min,,120r/min48h,COD。,。,。[12,13]1.2.2　高效菌株对高盐度采油废水的降解实验。1∶1∶1∶1,。1mL400mL0.1Mpa、120℃30min,120r/min,12h、24h、48h、72h、96h、144h50mL,1000rpm20min、COD,。1.2.3　高效菌株对含有不同浓度石油的模拟采油废水的降解实验1mL100mL0.1Mpa、120℃30min,,100r/min96h,COD。,,3030nm。。1.2.4　高效除油细菌的诱变育种及正突变菌株的筛选,。106/mL5mL,(20W,253.7nm)30cm,0、10、20、30、40、60、120、360s,,,,,30℃3d。,,,。100mL0.1Mpa、120℃30min,100r/min96h,COD。COD,,。2　2.1　　　118,14。COD5,COD1,2。1　COD2　a28(Plesiomonas)a32(Plesiomonas)a52(Bacillaceae)16#(Sphaerotilus)Ts6(Bacillaceae)　　,,,。,,。2.2　a28、a32、a52、16#、Ts620.14mg/L,COD367mg/L。2、33。31钦州学院学报第24卷2　3　COD3　COD(mg/L)COD(mg/L)(%)(%)a2820.149.6452.0936746.0987.44a3220.1413.0035.44367162.5955.69a5220.1415.0925.06367222.4439.3816#20.1412.0640.10367119.9667.31Ts620.1414.7126.96367248.1032.3920.141.1494.3436728.1292.34　　a52、Ts6,COD,24～48h,。16#a28。25.06～52.09%,COD32.39～87.44%。,。94.34%,COD92.34%。2.3　5。COD4、5;COD3。4　(mg/L)a28a32a5216#Ts6114.6314.6314.6314.6314.6314.639.0511.5012.519.8713.384.06(%)38.1321.3414.4632.508.4772.24220.1420.1420.1420.1420.1420.1410.5814.7916.9913.0217.202.79(%)47.4326.5215.6035.3514.5689.82326.8426.8426.8426.8426.8426.8413.1419.1721.9716.0723.030.90(%)51.0328.5718.1140.1214.1796.64442.9642.9642.9642.9642.9642.9623.8630.3935.9027.3438.498.01(%)44.4529.2316.4236.3310.3881.34557.3057.3057.3057.3057.3057.3033.4943.9749.4639.5552.5712.20(%)41.5423.2513.6730.968.2478.70　　,。,。a2826.84mg/L51.03%;26.84mg/L96.64%。5　CODCOD(mg/L)a28a32a5216#Ts6124724724724724724788.89164.13191.90129.15206.9666.03(%)64.0133.5422.3047.7016.2073.2632第6期申泰铭,戴梓茹:除油菌的筛选及其处理采油废水的研究续表(mg/L)a28a32a5216#Ts6236736736736736736774.80213.87265.18149.22293.0132.56(%)79.6138.6827.7453.3622.3791.12349149149149149149172.71292.37345.25197.46381.9312.24(%)85.1840.4529.6859.7822.2197.504787787787787787787219.94454.68589.41364.36643.41137.95(%)72.0542.2225.1053.7018.2482.475104110411041104110411041314.96660.50788.02499.87857.16209.98(%)69.7436.5524.3051.9817.6579.82　　,CODCOD。COD。a28COD491mg/L85.18%;COD491mg/LCOD97.50%。96h,COD。COD4、5:4　5　COD6　96.64%。,COD97.50%,。COD,。,26.84mg/L96.64%;COD491mg/L97.50%。6,,。,16#,1820。72h,,,88.91%。2.4　,。100mL100r/min96h,COD。7、8:33钦州学院学报第24卷7　8　CODa28、a32、16#。13%,COD20.68%。,、。3　(1),。,25.06～52.09%,COD32.39～87.44%。(2)120h94.34%,COD92.34%,。,。(3)。26.84mg/L(96.64%);COD491mg/L(97.50%)。。,,72h,88.91%。(4)。a3216#,,a3213%,16#COD20.68%。[1],.[M].:,2002.[2],,.[J].,2000,19(3):24-29.[3],.[J].,2000,18(6):22-24.[4],.[J].,2003,4(12):12-16.[5],.[J].,1999,15(3):21-24.[6],.[J].,2005,24(5):68-71.[7],.[J].,2001.2(4):49-52.[8],.[J].,1999,12(3):22-27.[9],.[J].,2003,23(2):184-188.[10],.[J].,2000,19(11):529-531.[11],.[J].,2005,24(5):68-71.[12],.[M].:,2005.[13],.[M].:,2001.BreedingandScreeningAnaerobicBacteriumtoTreatWastewaterfromOilRefiningSHENTai-ming,DAIZi-ru(DepartmentofBiologyandChemistry,QinzhouUniversity,Qinzhou535000,China)Abstract:Throughtheacclimationofactivesludge,fivekindsofbacteriumwereacquired,whichhasaCODremovalratebetween32.39%and87.44%andapetroleumhydrocarbonremovalratebetween25.06%and52.09%.ThemixedbacteriahaveahighergrowthrateandithasamaximalremovalratetoCODandpetroleumhydrocarbonat92.34%and94.34%.Thesebacteriahasaremovalratetosaturated-hydrocarbonabout96.64%at26.84mg/L,andaremovalratetoCODabout97.50%at491mg/L.Andtothearyl-hydrocarbonhasamaximalremovalrateof88.91%.Keywords:Breedingbacterium;Biologicaltreatment;Highsalinitywastewater[　]34