处理低温污水耐冷菌生物膜的研究

282Vol.28.220072JournalofHarbinEngineeringUniversityFeb.2007韩晓云1,姜安玺2,贲岳2(1.黑龙江大学生命科学学院,黑龙江哈尔滨150080;2.哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090):,.,,,.30d,(COD)82%,(BOD)92%.10~30m,800mg,.,,..:;;;:X703.1:A:10067043(2007)02023704BiofilmcharacteristicsofpsychrotrophsforlowtemperaturesewagetreatmentHANXiaoyun1,JIANGAnxi2,BENYue2(1.CollegeofLifeScience,HeilongjiangUniversity,Harbin150080,China;2.SchoolofMunicipal&EnvironmentEngineering,HarbinInstituteofTechnology,Harbin150090,China)Abstract:Itisalwaysaproblemtotreatwintersewageeffectivelyincoldarea.Inordertoimprovethetreatmentefficiencyoflowtemperaturesewage,psychrotrophicbacteriaisolatedfromactivatedsludgewereappliedtofillingmaterials(SoftPolyurethaneFoam)andaddedintothesewagetreatmentsystem.After30dayscontinuousrunning,theCOD(ChemicalOxygenDemand)removalratewasimprovedto82%andBOD(BiochemicalOxygenDemand)removalratereachedto92%.PsychrotrophicbacteriainitiallyattachedtoSPFgraduallyformedmicrobialcommunitiessizingfrom10to30m,whichunevenlydistributedthroughouttheporousspaceoffillingmaterials.Therewerearound800mgbiomasspergramattachedtothefillingmaterials,thebiofilmsimmobilizedinouterlayerwereenrichedandmoreactivecomparedtothoseininnerlayer.Themicrobialstructuredevelopedaheterogeneouscolonizationpatternmainlycomposedofcocciandbacilli,andafewfilamentousmicroorganismsplayingaskeletalroleinthebiofilmformation.Themicrobialcharacteristicsoflowtemperaturebiofilmobservedaboveassuredthesewagetreatmentrunningstablyandefficiently.Keywords:lowtemperaturesewage;psychrotrophs;immobilization;biofilm:20060707.:(11511287).:(1970-),,,Email:[e-mail].,,.,,,.[1-2].[3],[4].,.1试验装置与方法112,6615L,05t/d.,,,,.1-,2-,3-,4-,5-,6-,7-,8-,9-,10-,11-,12-.1Fig1Experimentalequipmentoflowtemperaturebioreactor12,1.1Table1WaterqualityforexperimentsCOD/mg!L-1BOD5/mg!L-1/∀pHDO/mg!L-1144~875150~2680~870~824~813,0~10∀,4~5∀.CODBOD(SS,VSS).,25%6h,HitachiS520.14[5].(SPF),40~60mm,006kg/L,90%~98%,(PVA),.,LB,4∀n=110r/min2d,3,10%,15∀pH70125r/min1(OD65015).10%PVA,,,,24h,.10%,30%.2试验结果与讨论21,,,,.,.,COD.2,COD30%80%,40d.,7h4∀,COD826%,COD60mg/L(3).BOD172~268mg/L,BOD20mg/L,90%(4).!238!282CODFig2CODremovalbyimmobilizedpsychrotrophs3CODFig3CODremovaloflowtemperaturebioreactor4BODFig4BODremovaloflowtemperaturebioreactor22,,,1/3,,.5.30d,800mg,,,VSS/SS.,..6,,,.5Fig5Biomassdistributiononfillingmaterialpergram6(#60)Fig6Biofilmimmobilizedonfillingmaterial(#60)!239!2,:237.,10~30m,.,.,.,O2[6],,.,,,.7Fig7Microbialcommunitiesinlowtemperaturebiofilm,,.,,.3结论1),,,30d.COD82%,BOD92%.2)10~30m,800mg,;,.3),,.:[1]RIVASIM,ARVINF.Sidestreambiofilmforimprovedbiofoulingcontrolincoolingwatersystem[J].WatSciTech,2001,41:445-451.[2]PAULLB,TONGY.Amicroelectrodestudyofredoxpotentialchangeinbiofilms[J].WatSciTech,1999,39(7):179-185.[3]MASSOLDEYAAA,WHALLONJ,HICKEYRF,etal.Channelstructuresinaerobicbiofilmsoffixed-filmreactorstreatingcontaminatedgroundwater[J].ApplEnvirMIcrol,1995,61(3):769-777.[4]CHRYSISL,BRUCEER.Modelingthedevelopmentofbiofilmdensityincludingactivebacteria,inertbiomass,andextracellularpolymericsubstances[J].WaterResearch,2004,38:3349-3361.[5]JIANGA,MENGX,CAOX,etal.Isolationofpsychrotrophsandtheirapplicationtotreatmentofsewageatcoldarea[J].JournalofHarbinInstituteofTechnology,2002,34(4):563-565.[6]EBERLHJ,PICIOREANUC,HEIJINENJ,etal.Athreedimensionalnumericalstudyonthecorrelationofspatialstructure,hydrodynamicconditions,andmasstransferandconversioninbiofilms[J].ChemEngSci,2000,55:6209-6222.[:]!240!28