处理垃圾渗滤液的SBR中微生物种群与污泥比阻蔡丽云1

39220182ENVIＲONMENTALSCIENCEVol．39No．2Feb．2018SBＲ363105SBＲSBＲ1SBＲ2．SBＲ130～50d35dSBＲ240d．50dSBＲ1Ｒozellomycota83.71%．SBＲ2BasidiomycotaTrichosporon99.84%99.78%．SBＲ1SBＲ2ThaueraSBＲ139.35%Planktosalinus、ThaueraOttowiaSBＲ216.84%、16.23%12.55%．SBＲ2SBＲ1．SBＲX172X703.1A0250-3301201802-0880-09DOI10．13227/j．hjkx．2017072022017-07-252017-10-29JAT1606481986～[e-mail]ndSludgeSpecificＲesistanceinTwoSBＲsTreatingLeachateCAILi-yunHUANGZe-binXUZi-weiJIANGZhi-binLINLi-liHUANGYuFujianKeyUniversityLaboratoryofEstuarineEcologicalSecurityandEnvironmentalHealthSchoolofEnvironmentalScienceandEngineeringofXiamenUniversityTanKahKeeCollegeZhangzhou363105ChinaAbstractTherelationshipbetweenmicrobialpopulationsandsludgefiltrationperformancewasstudiedwhenactivesludgewasusedtotreattheleachatefrommunicipalsolidwasteincinerationplants．TwoSBＲsSBＲ1andSBＲ2wereoperatedatthesameconditionsexceptthatSBＲ1wasexposedtothesunlightandSBＲ2wasinthedark．Toidentifythedifferenceinmicrobialpopulationsintworeactorshigh-throughputsequencingmethodwasused．Onthe50thdaythefungiabundanceinSBＲ2washigherthaninSBＲ1．PhylumＲozellomycotabecamethedominantfungiinSBＲ1whoserelativeabundancewas83.71%．PhylumBasidiomycotaandGenusTrichosporonbecameonlydominantfungiinSBＲ2whoserelativeabundanceswere99.84%and99.78%respectively．BacterialabundanceinSBＲ1washigherthaninSBＲ2．InSBＲ1Thauerawasthemajorbacterialgenuswhoserelativeabundancewas39.35%．InSBＲ2PlanktosalinusThaueraandOttowiawerethemajorbacterialgenerawhoserelativeabundanceswere16.84%16.23%and12.55%．Ｒotifersandotherpredatorymetazoanweredetectedonthe30th-50thdaysinSBＲ1andsludgespecificresistancebegantodeclineonthe35thday．FilamentousfungibulkingcausedbyTrichosporonresultedinacontinuousincreaseinsludgespecificresistanceofSBＲ2．ThedominantmicrobialcommunitiesespeciallyfungiandsludgespecificresistancesinSBＲ1andSBＲ2wereverydifferentbecauseoftheeffectofsunlight．Thereforesunlightplaysanimportantroleonmicrobialcommunitiesandsludgecharacteristics．KeywordsleachateSBＲsludgespecificresistancemicrobialcommunitysunlightCOD15000～20000mg·L－1BOD5/COD0.3～0.51500～2000mg·L－11．“+”2．．．Ishizaki3MBＲ、．4Penicillium、Trichosporon．52SBＲ．．MBＲ6．7．．pH8．．．DNA9．SBＲSBＲ1SBＲ2SBＲ1SBＲ2．．11.1SBＲ30L．SBＲ1SBＲ2、．SBＲ1SBＲ220h3.5h0.5h．4.5～5.6mg·L－1pH7.6～8.620～25℃COD/MLSS0.12～0.24g·g·d－1．SBＲ1SBＲ2．1.2．COD30618～36456mg·L－1NH+4-N947～1868mg·L－1pH7.0～7.518～23℃．1～2d20h30L．3d20h1∶10∶．10d20h2∶9．．1.3CODNH+4-N10．pH、DOWTWMulti3400WTWLaborprodukteGEＲPJK02．1.4DNAPCＲSBＲ1SBＲ250dSBＲ1SBＲ2DNADNAFastDNASPINKitforSoilQiagenCAUSA．338F5'-ACTCCTACGGGAGGCAGCAG-3'806Ｒ5'-GGACTACHVGGGTWTCTAAT-3'16SrＲNAV3V4PCＲ95℃3min2795℃30s55℃30s72℃30s72℃10min．ITS1F5'-CTTGGTCATTTAGAGGAAGTAA-3'ITS2Ｒ5'-GCTGCGTTCTTCATCGATGC-3'ITSPCＲ95℃3min3595℃30s55℃30s72℃30s72℃10min．PCＲGeneAmp9700ABI20μL4μL5×FastPfu2μL2.5mmol·L－1dNTPs0.8μL5μmol·L－10.4μLFastPfu10ngDNA．1.5IlluminaMiSeq2%PCＲ18839AxyPrepDNAGelExtractionKitAxygenBiosciencesUnionCityCAUSATris-HCl2%．QuantiFluorTM-STPromegaUSA．IlluminaMiSeqIlluminaSanDiegoUSAPE2×300．IlluminaMiSeqPE300．UPAＲSEversion7.1http．//drive5．com/uparse/97%OTUUCHIME．ＲDPclassifierhttp．//rdp．cme．msu．edu/16SSilvaＲelease128http．//．arb-silva．deITSUniteＲelease6.0http．//unite．ut．ee/index．php70%．22.1SBＲ1SBＲ2COD496～800mg·L－1COD60%～78%．SBＲ1SBＲ2NH+4-N10～20mg·L－185%～95%．SBＲ1SBＲ2．3.77×1012m·kg－1．SBＲ1SBＲ21．．1SBＲ135d．SBＲ2．SBＲ130～50d35dSBＲ240d2．1SBＲ1SBＲ2Fig．1SludgespecificresistanceinSBＲ1andSBＲ2aSBＲ1×10bSBＲ2×40250dSBＲ1SBＲ2Fig．2MicroscopicpicturesofsludgeinSBＲ1andSBＲ2onthe50thday2.2SBＲ1SBＲ22.2.1SBＲ1SBＲ216SrＲNA74085．97%SBＲ1SBＲ2321OTUs．3．4VennSBＲ1SBＲ2OTUs207OTUs64.48%．SBＲ12882SBＲOTUs21.81%SBＲ213.71%．3Fig．3Shannoncurvesofbacterialcommunities1SBＲ1SBＲ2α．ChaoShannonSimpson4VennFig．4Venndiagramofbacterialcommunities．SBＲ1Chao287.5SBＲ2259SBＲ1．SBＲ1Simpson0.143SBＲ20.07SBＲ1Shannon3.292SBＲ23.472SBＲ1SBＲ2．1αTable1Theα-diversityindicesofbacterialcommunities/%OTUsChaoShannonSimpsonSBＲ19734150277287．53．2920．1430．999SBＲ297399352512593．4720．0700．9992.2.2OTUs．＞1%5ProteobacteriaBacteroidetesSBＲ164.27%27.21%SBＲ257.92%37.16%．FirmicutesSBＲ22.75%SBＲ10.52%PlanctomycetesTM6SBＲ11.33%2.62%SBＲ2．SBＲ1SBＲ2．1%6．SBＲ11580.07%．β-ＲhodocyclaceaeThauera39.35%Flavobacteriales6.91%Cytophagaceae5Fig．5PercentofbacterialcommunityabundanceatthephylumlevelinSBＲ1andSBＲ26.14%NS9_marine_group5.32%．SBＲ21684.31%．388396Fig．6PercentofbacterialcommunityabundanceatthegenuslevelinSBＲ1andSBＲ2FlavobacteriaceaePlanktosalinus16.84%Thauera16.23%β-ComamonadaceaeOttowiaBrachymonas12.55%8.04%γ-XanthomonadaceaePseudofulvimonas7.49%NS9_marine_group3.49%．CytophagaceaeSBＲ1Planktosalinus、Ottowia、BrachymonasPseudofulvimonasSBＲ2．6SBＲ1Thauera．SBＲ2ＲhodocyclaceaeFlavobacteriaceaeComamonadaceae．SBＲ1SBＲ2、．β-Thauera、OttowiaBrachymonasPlanktosalinus1112．Thauera13．Thauera6014．Thauerasp．strainTN9COD13．Thauera11．SBＲ1SBＲ2Thauera．1015．ThaueraSBＲ1SBＲ2SBＲ1．16FlavobacteriumSBＲ2FlavobacteriumSBＲ2．SBＲ2Ottowia3OttowiathiooxydansN2O17Ottowiapentelensis18OttowiabeijingensisAN3T19．SBＲ2BrachymonasnirS20SBＲ2NO－2．SBＲ2Pseudofulvimonas2122．124882SBＲ．23．．Thauera、OttowiaBrachymonasSBＲ1SBＲ2．Cyto