畜禽废水处理下橐吾的生长和生理特性王应军

20112540812～0817JournalofNuclearAgriculturalSciences2011-02-052011-04-2910ZA0591972-、、。[e-mail]1000-8551201104-0812-066250142Ligulariasibirica。9.40%6.51%14.24%1.94%12.94%2.31%62d5651dPODMDA、、CO2＞＞。。GROWTHANDPHYSIOLOGICCHARACTERISTICSOFLigulariasibiricaUNDERLIVESTOCKWASTEWATERTREATMENTWANGYing-junZHANGJun-pingDENGShi-huaiCollegeofResourcesandEnvironmentSichuanAgriculturalUniversitySichuanYa’an625014AbstractEffectsofwastewateronthegrowthandphysiologiccharacteristicofLigulariasibiricawerestudiedthroughsimulatingconstructedwetland．ResultshowesthattheplantheightofLigulariainhigherandmiddleconcentrationlivestockwastewatertreatmentincreasedby9.40%and6.51%leaflengthincreasedby14.24%and1.94%andtheleafwidthincreasedby12.94%and2.31%comparedtothecontrolgrouprespectively．TheflorescenceofLigulariainhigherconcentrationtreatmentgroupreached62dwhilethemiddleconcentrationtreatmentgroupandthecontrolwereonly56and51d．BothPODactivityandrootactivitydecreasedatfirstandincreasedlater．ThevariationofMDAconcentrationperformedanirregulartrendbutitwaslowerthancontrol．Themembraneshowednoobviouschangeofelectrolyteleakagephenomena．ThenetphotosyntheticratestomatalconductanceintercellularCO2concentrationandtranspirationratefollowedtheorderofhigherconcentration＞middleconcentration＞control．ThereforeitcanbeconcludedthatLigulariahasgoodadaptabilityandpollutionresistanceabilitytolivestockwastewateranditisanexcellentconstructedwetlandplant．KeywordsLigularialivestockwastewatergrowthornamentalqualityphysiologiccharacteristic、1。N、P。、、2184N、P、234。5。Ligulariasibirica373～2200m、、、、、、、、、、、、、。6。。、、7～9。。、310～12、、913～14。。11.1。。1、、、、。1Table1TheeffluentwaterqualityparameterofgridindexCODTPTNNH+4-Nconcentrationmg/L309.6～598.410.36～17.1846.42～91.1943.31～69.501.233cm26cm29cm3～5cm5cm1～2cm5cm10cm6kg5cm3kg。1.3COD＜200mg/LTP＜5mg/LTN＜50mg/LNH+4－N＜30mg/L155。16。2T11T21CK。35。COD=238.4mg/LTP=15mg/LTN=36.28mg/LNH+4－N=36.28mg/L1T1T27d。2.2L7d1。4。1.41.4.147d、、。17。1.4.27d、3～5POD、1～2cm。POD18A4700.011PODTBA1919TTC20。Pn、Gs、Ci、TrLi－6400。900－11005。1.5Excel2003SPSS16.0One-WayANOVALSD。3182522.1、。23、T1＞T2＞CK。T1T2CK9.40%6.51%14.24%1.94%12.94%2.31%。7CKP＜0.013P＞0.05。3P＞0.0128T1CKP＜0.0542T1T2P＜0.05T2CKP＞0.05。721T1T2、CKP＜0.0135T1CKP＜0.05T2CK14P＜0.05。、T1T2。2Table2ThegrowthstatusofLigulariaunderdifferenttreatmentscmtreatedtimedplantheightleaflengthleafwidthCKT2T1CKT2T1CKT2T1724.41Bb28.43Aa29.38Aa16.20Aa14.88Ab16.98Aa13.24Bb13.41Bb15.05Aa1428.57Aa30.5Aa31.47Aa15.98Aa15.93Aa18.45Aa13.45Ab14.13Aa15.49Aa2127.97Aa28.8Aa29.92Aa16.05Aa16.18Aa17.83Aa14.2Bb14.26Bb15.48Aa2830.12Aa30.95Aa32.41Aa15.20Ab16.25Aab18.10Aa13.76Bb13.94ABb15.36Aa3528.19Aa29.22Aa29.06Aa14.13Ab16.00Aab17.35Aa12.84Aa13.12Aa14.6Aa4226.74Aa28.9Aa29.36Aa15.17Ab15.30Ab17.2Aa13.2Bb13.68ABb15.13Aaaverage27.6729.4730.2715.4515.7517.6513.4513.7615.190.010.05。CKT11T2T11。NoteDatainthesamerowwithdifferentcapitallowercaselettersweresignificantlydifferentatP=0.010.05thesameasfollowingtablesandfigures．CKtapewaterT1wastewaterwhichcontentassameasintable1T2T1dilutedonetime．、。、。42T2CKCKT1。3、T162dT2CK5651d。2.22.2.1PODH2O2H2O821。POD。3PODT247.42U·g－1·min－1＞T138.38U·g－1·min－1＞CK37.56U·g－1·min－1。1－AT1POD21POD19.88U·g－1·min－1CK55.40%POD42CK1.7CKT2P＜0.01。T2PODCK2160.63U·g－1·min－1CK1.697T2PODP＜0.01CK。T1T235P＞0.05。2POD。2.2.2MDA22。MDA、23。1－B。T141841POD、MDA、Fig．1PODactivityMDAconcentrationmembranepermeabilityandrootactivitychangeofLigulariaunderlivestockwastewatertreatment21dCK28dCKCK1.123542CK。282。T2CK2128dCKP＜0.05T128dP＞0.05。282T1。2.2.3。20。3CK19.34%＞T119.06%＞T218.41%。1－C21T1、T2CK3P＞0.05。2T11423.50%CKP＜0.01CK19.48%1.2114～28T128T1CK。T214CKCKT114dT1P＜0.01P＞0.05。T2T1。2.2.4。。3T25.55μg·g－1FW－1·h－1＞T14.28μg·g－1FW－1·h－1＞CK3.86μg·g－1FW－1·h－1。1－DT114CKP＜0.01213.28μg·g－1FW－1·h－1CK83.46%CKP＞0.055182528T1CK28d。T2145.06μg·g－1FW－1·h－1CK87.67%P＞0.0514T2CKT17T2T1228P＜0.01。T2T1。2.2.5、、、。。3CKP＜0.01。29P＜0.01。、。。T1CK17P＞0.05P＜0.01。T217CK。3。3Table3ChangesofphotosyntheticcharacteristicofLigulariaunderthelivestockwastewatertreatmenttreatedtimednetphotosyntheticrateμmolCO2·m－2·s－1stomatalconductancemolH2O·m－2·s－1CO2intercellularCO2concentrationμmolCO2·m－1transpirationratemmolH2O·m－2·s－1CKT2T1CKT2T1CKT2T1CKT2T126.87Cc8.69Bb10.56Aa0.12Bb0.15Bb0.28Aa253.27Bb254.13Bb280.13Aa2.86Bc3.59Bb5.46Aa912.69Bb13.27Bb14.68Aa0.33Bb0.54Aa0.52Aa261.40Bb298.96Aa290.36Aa3.09Bb4.35Aa4.28Aa1712.93Bb13.56ABab13.98Aa0.46Aa0.34Bb0.53Aa295.30Bb275.16Cc320.08Aa5.24Aa4.21Bb5.43Aa316.1Bb7.37Aa7.56Aa0.22Cc0.46Aa0.37Bb271.28Bc301.52Aa292.64Ab2.81Bc4.44Aa3.97Ab487.8Bb8.14ABab8.74Aa0.25Bc0.32Bb0.53Aa333.54Cc341.13Bb358.14Aa3.91Cc4.62Bb5.41AaCO2。。3CO23T1308.27μmolCO2·m－1＞T2294.18μmolCO2·m－1＞CK282.96μmolCO2·m－1。CO2CO2。3。TN54.5mg/LNH+4－N24.7mg/L24。TN91.19mg/LNH+4－N69.50mg/L。POD。MDA。。、。POD。MDA。。。、、CO2。CO2。25。。618JournalofNuclearAgriculturalSciences20112540812～0817T1T27。T1POD、。4。2。1．J．2001410－132．J．20082018－113．J．20083485－84．J．200726441－4465YangQChenZHZhaoJGetal．ContaminantremovalofdomesticwastewaterbyconstructedwetlandseffectsofplantspeciesJ．JournalofIntegrativePlantBiology2007494437－4466．P．20101012281