次氯酸钠对扁浒苔叶绿素荧光特性及光合速率的影响

1674－5566201402－0215－072013-10-152013-12-052012BACD7B032012050122011050231988—。[e-mail][e-mail]121212121121．2013062．201306Ulvacompressa、、。NaClO10．0mmol/LNaClOab0．030mg/g0．039mg/g0～6．00mmol/LNaClOFv/Fm10．0～100．0μmol/L3min100μmol/L1315nmolO2/min·g－472nmolO2/min·g。4．0mmol/LNaClO。。5cm。S917AUlvacompressaChlorophyta、Ulvales、Ulvaceae、UlvaUlvalinza、Ulvaprolifera。、、、。1241。200720137。23。2009－201224－5。Porphyrayezoensis6－7。NaClO、、89－11。NaClONaClO、。11．1U．compressa。2320．5±0．5℃、20±0．5、60±5μmol/m2·s、12D∶12LVES。PSⅡFv/Fm0．6。1．25．2%NaClO2．00、4．00、6．00、8．00、10．00mmol/L0mmol/L。5cm1．00g100mLNaClO5min3。1．3。0．5g5mL5mL80%4200×g15min80%25mL。T6。80%350～700nm。663nma645nmb。aCabCb12mg/L。mg/g。Ca=12．7A663－2．69A6451Cb=22．9A645－4．68A66321．4PHYTOPAMPhytoWin13。5minPhyto-PAM520nm0．1μmol/m2·s。PhytoWinMLFv/Fm。5minPSⅡYield。1．5NaClO0．05gFW20℃OxygraphHansatechNaClO10．0、20．0、40．0、60．0、80．0、100．0μmol/L600μmol/m2·s。nmolO2/min·g。22．11a436nm663nmb463nm645nm。680nm。ab。1Fig．1TheabsorptionspectraofthechlorophyllextractedfromU．compressa2．2NaClO2NaClO1NaClOab2。2．00mmol/LNaClONaClOChl．a、Chl．bChl．a/Chl．b10mmol/L6122a91．10%b79．69%。2NaClOFig．2CellsurvivalofU．compressaafterNaClOtreatmentA．B．。1NaClOTab．1ChangeofchlorophyllcontentafterdifferentconcentrationNaClOtreatmentNaClO/mmol/LChl．a/mg/gChl．b/mg/gChl．a/Chl．b00．337±0．0140．192±0．0091．75±0．0282．000．206±0．0040．136±0．0051．52±0．0774．000．125±0．0070．115±0．0061．09±0．0596．000．106±0．0110．114±0．0060．93±0．1418．000．054±0．0060．066±0．0030．82±0．06110．000．030±0．0010．039±0．0040．77±0．0582．3NaClO3Fv/FmYeildNaClOFv/Fm0．644．00mmol/LFv/Fm0．110．0mmol/L。3NaClOFig．3ComparationoffluorescencecharacteristicsofU．compressaafterNaClOtreatment2．4NaClO、。4NaClO10．0μmol/L3～19min80．0μmol/L100．0μmol/L7min。4NaClO-Fig．4Ｒelationshipoftime-photosyntheticratewithdifferentconcentrationNaClOtreatment3min5NaClONaClO10．0μmol/LNaClO1300nmolO2/min·g570nmolO2/min·g10．0～100μmol/L3．85%～4．28%。5NaClO3minFig．5Thechangeofphotosyntheticrateafter3minofdifferentconcentrationNaClOtreatment712233NaClO14pH。15NaC1ONaCIO。NaClONaClO。、PSⅡPSⅠATPNADPH1617a、NaClO14a18。Chl．a/Chl．bPSⅡLHCⅡChl．a/Chl．b1．0～2．319LHCⅡ20。NaClO10mmol/LChl．a/Chl．b0．77LHCⅡ。2122。Fv/FmPSⅡPSⅡ23－24Fv/Fm0．6525。Fv/Fm26NaClOFv/FmNaClO。NaClO10mmol/LNaClOPSⅡPSⅡ27LHCⅡ。ＲOSＲOS2829ＲOS。。30。、、31、/、1cm。28Fv/Fm20%Fv/Fm60%。NaClO。NaClOOO812214。NaClO。NaClONaCl、NaClO3。NaClO32－35NaClO。36pH37pHpH12pH38。4．2、5．6、7．0mmol/L37．0mmol/LNaClO30min4．25．6mmol/LNaClO3d4．25．6mmol/L4．2mmol/LNaClO。NaClO。NaClO、NaClO、。1．J．200832813－15．2．J．2011564/5309－317．3OWENSANJPSTEWAＲTWDP．EnteromorphaandthecyclingofnitrogeninasmallestuaryJ．EstuarineCoastalandShelfScience1983173287－296．4PANGSJLIUFSHANTFetal．TrackingthealgaloriginoftheUlvabloomintheYellowSeabyacombinationofmolecularmorphologicalandphysiologicalanalysesJ．Marineenvironmentalresearch2010694207－215．5WANGJFJIANGPCUIYLetal．Molecularanalysisofgreen-tide-formingmacroalgaeintheYellowSeaJ．AquaticBotany201093125－31．6．J．19951728293．7．J．1996210－11．8．J．20021618－10．9．/J．2007373245－248．10．J．199716228－30．11．J．2008295130－136．12AＲNONDI．Cooperenzymesinisolatedchloroplasts．PolyphenoloxidaseinBetaVulgarisJ．PlantPhysiol19492411－15．13GEＲAAALCOVEＲＲOTMASCAＲOOetal．ExploringtheutilityofPosidoniaoceanicachlorophyllfluorescenceasanindicatorofwaterqualitywithintheEuropeanWaterFrameworkDirectiveJ．Environmentalmonitoringandassessment201218463675－3686．14．pHJ．2011391710353－10355．15．J．20093411－13．16．J．200413169－75．17LICHTENTHALEＲHKACAMAＲEKMVetal．DifferencesinpigmentcompositionphotosyntheticratesandchlorophyllfluorescenceimagesofsunandshadeleavesoffourtreespeciesJ．PlantPhysiologyandBiochemistry2007458577－588．18．J．2006225829－832．19ＲOWANKS．PhtotsyntheticpigmentsofalgaeM．CUPArchive1989．20．J．200326s189－92．21SCHＲEIBEＲUBILGEＲWNEUBAUEＲC．Chlorophyll91223fluorescenceasanon-intrusiveindicatorforrapidassessmentofinvivoPhotosynthesisM．EcophysiologyofPhotosynthesisEcologicalStudies．BerlinSpringer-Verlag199449－70．22SCHＲEIBEＲU．Pulse-amplitude-modulationPAMfluorometryandsaturationpulsemethodanoverviewM．ChlorophyllaFluorescent．SpringerNetherlands2004279－319．23GUODPGUOYPZHAOJPetal．PhotosyntheticrateandchlorophyllfluorescenceinleavesofstemmustardBrassicajunceavar．tsatsaiafterturnipmosaicvirusinfectionJ．PlantScience2005168157－63．24GENTYBBＲIANTAISJMBAKEＲNＲ．TherelationshipbetweenthequantumyieldofphotosyntheticelectrontransportandquenchingofchlorophyllfluorescenceJ．BiochimicaetBiophysicaActa-GeneralSubjects1989990187－92．25BISCHOFKHANELTDWIENCKEC．UV-radiationcanaffectdepth-zonationofAntarcticmacroalgaeJ．MarineBiology19981314597－605．26LIMAJDMOSQUIMPＲDAMATTAFM．LeafgasexchangeandchlorophyllfluorescenceparametersinPhaseolusVulgarisasaffectedbynitrogenandphosphorusdeficiencyJ．Photosynthetica1999371113－121．27．J．2004245812－816．28DUMMEＲMUTHALKAＲSTENUFISCHKMetal．Ｒesponsesofmarinemacroalgaetohydrogen-peroxidestressJ．JournalofExperimentalMarineBiologyandEcology．20032891103－121．29．M．20033－4．30．J．199215．31．J．2005256103－105．32．J．200739912－14．33．J．2007193202－204．34．J．20053438－10．35．D．200816－17．36SIＲTESGWALTIMOTSCHAETZLEMetal．Theeffectsoftempe