单级生物脱氮的特性研究曹国民

21320005　　　　　　ENVIRONMENTALSCIENCEVol.21,No.3May,2000*,,,(,　200237,E-mail:[e-mail]):PVA,,、、pH、、DO.:/=1.5∶1～3.6∶1(W∶W),.、、4,.、pHDO30℃、8.22mg/L～6mg/L.,,/9.0,.:,,,.:X172,X703.1　:A　:0250-3301(2000)03-0040-04*　(ProjectSupportedbytheNationalNaturalScienceFoundationofChina):29777007:(1963～),,.:1999-09-11CharacterizationofSingle-StageBiodenitrification*GuominCao,QingxiangZhao,JianliGong,TongZhang(DepartmentofEnvironmentalEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,ChinaE-mail:[e-mail])Abstract:Theeffectsoftheratioofnitrifyingbacteria/denitrifyingbacteria,organiccarbonsource,pH,alkalini-ty,temperatureandDOonsingle-stagebiodenitrificationprocessbycoimmobilizednitrifyingbacteriaanddeni-trifyingbacteriawerestudiedinthispaper.Theresultsshowedthatthetotalinorganicnitrogen(TIN)removalratewasthequickestwhiletheratioofnitrifyingbacteria/denitrifyingbacteriawasbetween1.5/1and3.6/1(W/W)andorganiccarbonsourcewasethanoloutofmethanol,aceticacidandglucose.TheoptimalpH,DO,andtemperaturewasabout8.2,2mg/L～6mg/Land30℃respectively.Theactivationenergyofthesingle-stagebiodenitrificationprocesswascalculatedas28.6kJ/mol.TheTINremovalrateincreasedastheratioofalkalini-ty/ammonianitrogenincreased,butwhentheratioofalkalinity/ammonianitrogenwasover9.0,theTINre-movalratetendedtobestable.Keywords:biodenitrification,nitrifyingbacteria,denitrifyingbacteria,immobilization.　　10.[1～6],11300m3/d[7].,,[8],.PVA,[9],、、pH、、,.1　1.1　.:(NH4)2SO4700mg/L,NaHCO31000mg/L,、;:KNO31800mg/L,CH3OH1500mg/L,.1.2　DOI:10.13227/j.hjkx.2000.03.009(PVA),1750±50,;(),..1.3　[9],3000r/min15min,2.8%PVA2%,4%CaCl2,、30min,3mm.CaCl2,,-15℃,.4,.1.4　(NH4)2SO4300～500mg/L,NaHCO3600～1000mg/L(),,100mol/L、pH8.2(pH),CH3OH340～570mg/L().1.5　1L(5.1cm,50cm)(30±1℃,),15%,24h..:20g200ml1000ml,100r/min、25℃24h.NH+4-N、NO-3-NNO-2-N,(TIN):TIN=NH+4-N+NO-3-N+NO-2-N=(TIN0V-TIN1V)/Vt:V;TIN0,TIN1、;t.NH+4-N、NO-3NNO-2-N,[10].2　2.1　pHpH.pH8.0～8.4,pH7.0～7.5[11].,,pH,1.1,pH8.2,pH,pH.,.pHpH.1　pH　　2(CaCO3,).2,,/9.0,.,,.pH,.SakairiMAC[12],/8.85,,,..,/9,,,,.2.2　BOD,3.3,30℃,413　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　.,10℃,30℃27%[13].,10℃,30℃46%.Arrhenius28.6kJ/mol,[14]63.6kJ/mol,.,.2　/2.3　,,;,,.,3　.,.,,,DO,DO,1.DO1～2mg/L,,DO2～6mg/L,,DO6mg/L,.DO,,;DO,DO,,DO,,,,.,NO-2-NNO-3-N.1　DO/mg·L-11～22～33～44～55～66～7/mg·(L·h)-13.434.164.254.654.203.922.4　.[15],.,[16].4:、、(3),,4.4,,,,、,.[17,18].,4　,4.,,.,,42　　　　　　21.2.5　,2,,7.5,,,1.5∶1～3.6∶1,.,,,.,(NO-2-NNO-3-N),TIN.5　3　(1),pH、8.2、30℃2mg/L～6mg/L.(2),./9,.(3),.,、,.(4),,.1.5∶1～3.6∶1,.,.1　SuminoT,NakamuraHandMoriNetal.Immobilization　　ofnitrifyingbacteriabypolyethyleneglycolprepolymer.J.Ferment.Bioeng.,1992,73(1):37～42.2　,..,1997,18(2):18～20.3　ChenKCandLinYF.Immobilizationofmicroorganismswithphosphorylatedpolyvinylalcohol(PVA)gel.En-zymeMicrob.Technol.,1994,16(1):79～83.4　TramperJ,Suwinska-BorowiceGandKlapwijkA.Char-acterizationofnitrifyingbacteriaimmobilizedincalciumalginate.EnzymeMicrob.Technol.,1985,7(4):155～160.5　WijffelsRH,SchukkingGCandTramperJ.Characteri-zationofadenitrifyingbacteriumimmobilizedinK-car-rageenan.Appl.Microb.Biotechnol.,1990,34(3):399～403.6　,..,1999,20(1):39～42.7　TanakaK,SuminoTandNakamuraHetal.Applicationofnitrificationbycellsimmobilizedinpolyethylenegly-col.Prog.Biotechnol.,1996,11(immobilizedcells):622～632.8　DosSantosVAPM,BruijnesMandTramperJetal.Themagic-beadconcept:anintegratedapproachtonitrogenremovalwithco-immobilizedmicroorganisms.ApplMi-crob.Biotechnol.,1996,45(4):447～453.9　OkazakiM,HamadaTandFujiiHetal.Developmentofpoly(vinylalcohol)hydrogelforwastewatercleaning.I.Studyofpoly(vinylalcohol)gelasacarrerforimmobiliz-ingmicroorganisms.JAppl.PolymerSci.,1995,58(12):2235～2249.10　.,.:,1997.252～275.11　..:,1992.17～30.12　SakairiMAC,YasudaKandMatsumuraM.Nitrogenre-movalinseawaterusingnitrifyinganddenitrifyingbacte-riaimmobilizedinporouscellulosecarrier.Wat.Sci.Tech.,1996,34(7,8):267～274.13　...:,1993.201.14　WillkeTHandVorlopKD.NitrificationinPVALbeads:InfluenceofpHandtemperatureonnitriteoxida-tion.ProgBiotechnol.,1996,11(immobilizedcells):718～724.15　MatejuV,CizinskaSandKrejciJetal.Biologyicalwaterdenitrification—areview.EnzymeMicrob.Technol.,1992,14(3):170～183.16　,,..,1999,5(1):64～68.17　LewandowskiZ.Denitrificationbypackedbedreactorsinthepresenceofchromium.WaterRes.,1985,19(5):589～596.18　LemoineD,JouenneandJunterGA.Reductionofnitratebypseudomonasentrappedincompositteagarlayer/mi-croporousmembranestructures.Biotechnol.Letters,1988,10(6):399～402.433