氮磷缺乏对污泥沉降性能及丝状菌生长的影响杨雄1

20143CIESCJournal1040March2014653Vol.65No.3/1001244SVI200250mlg1SVI100mlg1SVI120mlg1N.limicolaIIType0092N.limicolaIDOI10.3969/j.issn.0438-1157.2014.03.038X703.1A04381157201403104009Influenceofnitrogen/phosphatedeficiencyonsludgesettleabilityandfilamentousgrowthYANGXiong,PENGYongzhen,GUOJianhua,TANGXiaoxue,SONGJichen,WANGShuying(KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentRecoveryEngineering,EngineeringResearchCenterofBeijing,BeijingUniversityofTechnology,Beijing100124,China)Abstract:Theinfluenceofnitrogen(N),phosphate(P)andN&Pdeficiencyonsludgesettleabilityandfilamentousgrowthwasinvestigatedinfoursequencingbatchreactors.TheresultsshowedthatPdeficiencyininfluentwouldcausedecreaseofpolyphosphate-accumulatingorganisms(PAOs)andinhibitthestoragecapabilityoffloc-formers.Moreover,theproportionofproteininextracellularpolymersubstances(EPS)decreasedandsludgevolumeindex(SVI)of200250mlg1wasobserved.NdeficiencyininfluenthadlessimpactonthegrowthofPAOsandthestoragecapabilityoffloc-formers.ProteininEPSwassignificantlyimproved,andSVIoflessthan100mlg1wasobservedunderthiscondition.Theshort-termdeficiencyofN&Pininfluentwouldcausedeteriorationofsludgesettleability,butnofurtherdeteriorationwasobservedafterlong-termdomestication.Noexcessivegrowthoffilamentsbutlargenumbersofsphericalfloc-formersexistedintheN&Pdeficiencysystem,resultinginSVIofaround120mlg1.ThedominantfilamentsforN,PandN&PdeficiencywereN.limicolaII,2013-05-262013-08-0519862012ZX07302002-06Receiveddate:2013-05-26.Correspondingauthor:Prof.PENGYongzhen,[e-mail]:supportedbytheNationalKeyScienceandTechnologySpecialProjectsofWaterPollutionControlandManagementDuringtheTwelfthFive-yearPlanPeriod2012ZX07302002-06andtheProjectofScientificResearchBaseandScientificInnovationPlatformofBeijingMunicipalEducationCommission.3/1041Type0092andN.limicolaI,respectively.Allofthesedominantfilamentshadlimitedeffectonsludgesettleability.Keywords:aerobic;nutrient;nitrogen/phosphatedeficiency;sludgebulking;filaments;sedimentation;molecularbiology[1-2]CNP3[3][2][4-5][6]11.14SBR200mm12L10.4m3h1DODO(250.5)90d46h10min110min180min50min10min6LHRT12h200mlSRT15d18002500mgL11SBRFig.1SBRconfiguration1influenttank2influentpump;3magneticstirrer;4sludgepump;5sludgetank;6automaticheater;7effluentvalve;8airvalve;9airflowmeter;10airpump;11effluenttank;12communicationline;13automaticcontrolsystem1.24SBR-1SBR-2SBR-3SBR-41[7]4SBRSBR-2~4SBR-14SBRA2OSVI150mlg16510421Table1CharacteristicofartificalwastewaterItemSBR-1SBR-2SBR-3SBR-4CH3COONa/mgL1COD=300mgL1384.38384.38384.38384.38NH4Cl/mgL1NH4-N=30mgL1114.64114.64KH2PO4/mgL1PO4-P=15mgL165.8165.81MgSO4·7H2O/mgL191.291.291.291.2CaCl2/mgL111.111.111.111.1traceelements[7]/mlL11111COD:N:P100:10:5100:10:0100:0:5100:0:01.3SVIMLSSMLVSSCODCr-β-PHAEPSPHA10020hOehmen[8]Agilent6890NAgilentDB-10.6molL1HCl1006hSVIMLSSMLVSSCODCr[9]EPS[10]20ml3000rmin115min1PBS40ml0.12ml36.5%41h8ml1molL1NaOH43h10000rmin1420min0.22mEPSEPSLowryDNA[10]3EPSFIOlympus_BX61Eikelboom[1]FISHPAOsGAOs[7][11]MP223MicrothrixparvicellaNLIMI91NostocoidalimicolaINLIMIII301NostocoidalimicolaIIHHYHaliscomenobacterhydrossisTNIThiothrixPAOmixPAO462PAO651PAO846PAOsGAOmixGAOOQ431GAOOQ989GAOs34d147090d83d4FISH422.1/2SVI145mlg14SBR1015dSBR-1SBR-3SBR-1SVI100mlg1SBR-3SVI100mlg1SBR-2SBR-430dSVI300mlg1SBR-2SVI200250mlg1SBR-4SVI120mlg134SBR83dSBR-1SBR-3SBR-32SBRSVIFig.2SVIprofilesindifferentSBRs3/1043SBR-2SBR-4SBR-2FI4SBR-412[12]SBR/SBR-1SBR-3SBR-1SBR-3PAOsFISHSBR-3PAOs14.7%SBR-1PAOs16%PAOs[13]SBR-3PAOsPAOsPAOsPAOs[2]SBR-3SBR-1SVI150mlg1SBR-2SVI200mlg13PAOsEPS2.2.2[1]3200mFig.3MicroscopicexaminationofdifferentSBRs(bar=200m)651044SBR-2SBR-22SBR-2FISHGAOs4%GAOsPAOsGAOsSBR-4SVI120mlg1SBR-4VSS/MLSS0.9FISHPAOs1%GAOs10%SBR-4GAOs2.2/2.2.1SBRCOD4SBR-3CODSBR-1COD86%COD40mgL1SBR-2SBR-4COD55%65%COD100mgL1483dSBR-1SBR-3PHA[5ac]SBR-2SBR-4[5(b)(d)]SBR-1SBR-3PHA4SBRCODFig.4CODconcentrationineffluentofdifferentSBRs5SBRPHAFig.5PHAandglycogenstorageperformanceofdifferentSBRs3/104574.01mgCOD(gVSS)168.18mgCOD(gVSS)130min120minPHA35.49mgCOD(gVSS)148.84mgCOD(gVSS)1SBR-1SBR-3CODSVISBR-1PHASBR-3SBR-1SBR-1PHASBR-3SBR-3Mino[14]PHA[15]TCA30%PHAPHASBR-2SBR-4PHAATPTCATCASBR-2SBR-4PHAPAOsPAOsPHASBR-3CODPHATCAATPSBR-2SBR-4PHASBR-1SBR-35[16]PHAPHASBR-2SBR-4PHA2.2.2EPS[17-19]EPSEPS24EPS83d[20]EPS2SBR-3SBR-24DNALiao[21]SVIEPSEPS2SBREPSTable2ContrastofEPScompositionindifferentSBRsReactorProtein/mg(gVSS)1Carbohydrates/mg(gVSS)1DNA/mg(gVSS)1EPS/mg(gVSS)1SVI/mlg1SBR-188±345±38±4140±998SBR-258±337±29±2105±2316SBR-3106±144±46±1157±588SBR-471±530±29±2112±8119651046EPSSVI2EPSEPS[22]Mg2+Ca2+[23]EPSEPSEPS2.3/[24]EPSEikelboomType0092Type0041M.Parvicella[1,25]83dFISH43Type0092Type0041M.ParvicellaType009283dSBR-1M.ParvicellaType0041[6(a)]Type0092SBR-2N.limicolaII[6(b)]FISH620μmFig.6Dominantfilamentsindifferentsystems(bar=20μm)3/1047ThiothrixType0092H.hydrossisSBR-3Type0092[6(c]ThiothrixType0041SBR-4N.limicola[6(d)]Type0092Thiothrix3SBRTable3FilamentsandFIindifferentSBRsReactorFilamentous