低CN比城市污水短程硝化特性及微生物种群分布徐浩

113Vol．11No．320173ChineseJournalofEnvironmentalEngineeringMar．2017514781342015－11－292016－02－181986—。[e-mail]*C/N1*11211．5100602．150090C/NSBＲSBBＲ2。30±1℃、pH=7.9～8.2、DO=1.0mg·L－1、29d2NH+4-N99%90%SBＲSBBＲAOBSBBＲSBＲ。PCＲSBＲAOBNOB2.3∶1。X703.1A1673-9108201703-1477-05DOI10.12030/j．cjee．201511206CharacteristicsandmicrobialpopulationsdistributionofshortcutnitrificationwithlowC/NratiourbansewageXUHao1*LIJie1LUOFan12SUIJun11．GuangzhouMunicipalEngineeringDesignandＲesearchInsitituteGuangzhou510060China2．SchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090ChinaAbstractTheinoculatedsludgeforthestudywascollectedfromthesecondarysedimentationtankofamunici-palwastewatertreatmentplant．ThecharacteristicsandmicrobialpopulationdistributionofshortcutnitrificationforurbanwastewaterwithlowC/Nwereresearched．MeanwhilethedifferencebetweenthenitrifyingpropertiesoftheSBＲandSBBＲreactorswasdiscussed．Theresultsshowedthatafter29daysofoperationatatemperatureof30±1℃pHof7.9to8.2andDOof1.0mg·L－1shortcutnitrificationcanbeachievedefficientlyandstablywiththeratesofammoniaremovalandnitriteproductionbeingabove99%and90%respectively．Com-paredtoSBＲtheconditionsinSBBＲwerefavorableforthegrowthofammoniumoxidizingbacteriaAOBwhichresultedintheenhancementoftherateofnitriteaccumulation．FromtheresultsofthepolymerasechainreactionPCＲtheratioofAOBandnitriteoxidizingbacteriaNOBwas2.3∶1inastableoperationSBＲsys-temwithshortcutnitrification．KeywordspartialnitrificationsequencingbatchreactorsegmentedaerationlowC/NratiourbansewageAOBNO－2-N。1975VOETS1--、、2-5。SHAＲON、OLAND、SHAＲON/ANAMMOX。DO1.0mg·L－130～35℃pH7.0～8.56-1011AOB。11。C/N。SBＲSBBＲC/N2NO－2-N2C/N。11.1600mm400mm1200cm230L1。10cm。SBBＲ15cm。1SBＲSBBＲFig．1SchematicdiagramofSBＲandSBBＲ1.2。CH3COONa、NaHCO3、NH4ClKH2PO4NH+4-N=40～60mg·L－1、COD=150～200mg·L－1、pH=7.9～8.2。ZnSO4·7H2O0.2mg·L－1H3BO30.10mg·L－1CuSO4·5H2O0.15mg·L－1MnCl2·4H2O0.4mg·L－1CoCl2·H2O0.2mg·L－1FeCl3·6H2O1.5mg·L－1NiCl2·6H2O0.19mg·L－1。1.310d16SrDNA。DNADNADNA。DNA6nanodrop2000DNA－20℃。87413C/N25μLDNA、、1μLmixturebuffer12.5μLH2O25μL。Touchdown94℃5min94℃1min65～49℃1min72℃45s201℃72℃7min94℃1min5550℃1min72℃45s1572℃7min。3。NCBIblast。1.4NH+4-NNO－2-NN-1--NO－3-N6DOWTWoxi330ipH、starter3cpHCODcr5B-3CCODMLSSTSSPortable。OＲPHQ-40d。1.53。1SBＲ、SBBＲMLSS=2000～2500mg·L－1/13h、、DO、OＲP、pH。22。3。2SBＲ11→→→→。12-14→41h+2h12h→+1.0h。30±1℃、pH7.9～8.2、COD150～200mg·L－1、NH+4-N40～60mg·L－1DO1.0mg·L－1。218dNO－2-NNO－2-N/NO－2-N+NO－3-N×100%90%。SBＲ、SBBＲ2、NO－2-N。2.12NO－2-N2SBＲSBBＲFig．2NitrogenconversionsofSBＲandSBBＲduringonecycle2SBＲSBBＲ0～7dAOBNOBNH+4-NAOBNO－2-NNOBNO－3-NSBＲNH+4-N0.805g6.55gNO－3-N0.69g4.72gSBBＲNH+4-N0.92g7.68gNO－3-N1.04g4.97g。NH+4-NNO－3-N28～18d2NH+4-NNO－3-NNO－2-N。2AOB9741113SBＲSBBＲFig．3NO－2-NaccumulationrateofSBＲandSBBＲNOBAOBNO－2-NSBＲSBBＲNO－2-N6.18.4mg·L－118.519.6mg·L－1NO－2-N90%319～29d2NH+4-NNO－2-N99%90%。SBＲ、SBBＲ2。SBＲSBBＲ2NH+4-NNO－2-NNH+4-N40～60mg·L－123SBBＲSBＲSBBＲ。2.22AOBSBBＲPCＲamoAnxrA。14。1Table1Semi-quantitativeanalysisofgenefunction/%amoA11.4nxrA4.8116.2%。AOB11.4%NOB4.8%2.3AOBNOB4Fig．4ElectrophoresisprofilesofamplificationproductsNH+4-N、NO－2-NNO－3-N。2.3SBBＲSBＲ15。SBBＲSBＲSBＲSBBＲSBＲSBBＲ/。4NH+4-N、NO－2-N、NO－3-N5。1～43、6、9、12h。4NO－2-N。SBＲSBBＲ2SBBＲNH+4-NNO－2-NSBＲ3SBBＲNO－2-N41.26mg·L－14NO－2-NSBＲ3NO－2-N27.39mg·L－1。SBBＲ08413C/N5NH+4-N、NO－2-N、NO－3-NFig．5ChangesofNH+4-NNO－2-NandNO－3-NduringdifferentperiodsofshortcutnitrificationSBＲ。31NH+4-NC/N30±1℃、pH=7.9～8.2、DO=1.0mg·L－1SBＲNH+4-N99%NO－2-N90%。2NH+4-N40～60mg·L－1。SBBＲSBＲSBBＲSBＲ。SBBＲSBＲ4。316.2%AOB11.4%NOB4.8%2.3。C/NAOB。1VOETSJPVANSTAENHVEＲSTＲAETEW．ＲemovalofnitrogenfromhighlynitrogenouswastewatersJ．WaterPollutionControlFederation1975472394-3982．J．200319121-53．SBＲJ．2008294931-9364．J．2006221939-435KOWALCHUKGASTIENSTＲAAWHEILIGGHJetal．Molecularanalysisofammonia-oxidisingbacteriainsoilofsuc-cessionalgrasslandsoftheDrentscheATheNetherlandsJ．FEMSMicrobiologyEcology2000313207-2156ＲUIZGJEISONDCHAMYＲ．NitrificationwithhighnitriteaccumulationforthetreatmentofwastewaterwithhighammoniaconcentrationJ．WaterＲesearch20033761371-13777TOKUTOMIT．Operationofanitrite-typeairliftreactoratlowDOconcentrationJ．WaterScienceandTechnology2004495/681-888CHANGCHHAOOJ．SequencingbatchreactorsystemfornutrientremovalOＲPandpHprofilesJ．JournalofChemicalTechnologyandBiotechnology199667127-389HELLINGACSCHELLENAAJCMULDEＲJWetal．ThesharonprocessAninnovativemethodfornitrogenremovalfromammonium-richwastewaterJ．WaterScienceandTechnology1998379135-14210．pHJ．20114210181-18511．/J．200121179-8312．SBＲJ．20042069-1113HEＲNEBＲINGCOHLSSONLANDＲEASSONMetal．InteractionbetweenthetreatmentplantandthesewersysteminHalmstadIntegratedupgradingbasedonrealtimecontrolJ．WaterScienceandTechnology1998379127-13414．J．2013452101-10515．SBBＲJ．201228712-161841