低温工厂化养殖水体氨氮处理微生物的初步研究陈中祥

21820058TransactionsoftheCSAEVol.21　No.8Aug.　2005陈中祥,曹广斌※,刘　永,蒋树义,韩世成(,150070)　:,,。,,;,。、——。,12℃,。。:低温养殖;氨氮处理;微生物;生物滤池:S949　　　　:A　　　　:1002-6819(2005)08-0132-05,,　,.[J].,2005,21(8):132-136.ChenZhongxiang,CaoGuangbin,LiuYong,etal.Preliminarystudyonammonianitrogentreatmentofmicroorganisminindustrializedculturewateratcoldtemperature[J].TransactionsoftheCSAE,2005,21(8):132-136.(inChinesewithEnglishabstract):2004-10-20　:2005-04-05::(1979-),,,。　,150070。Email:[e-mail]:(1957-),,,。　,150070。Email:[e-mail]0　　、。,。,,。、,,,,,,,,[1,2]。。、。——,。,,,,[3-8]。,37℃,20℃,15℃[9]。,,,20℃,。,。,,。1　1.1　1)(Rainbowtrout):10～12cm,100,16.2kg·m-3。2):81.8m,1m3。3):2～5mm。4):(NH4)2SO42.0g、NaH2PO40.25g、MnSO4·4H2O0.01g、K2HPO40.75g、MgSO4·7H2O0.03g、CaCO35.0g、H2O1000mL。5):20g、KNO32.0g、MgSO4·7H2O0.03g、K2HPO40.5g、H2O1000mL,pH7.2。1.2　62。,。,,[10]。。1)。2004128,。218,132,3d。2)、pH、NH+4-N、NO-2-N、NO-3-N、CODBOD。[11]。、H+,pH,NaHCO3NaOHpH,。pH7.0,pH。3)1g,10mL,Vertex2min,5min,10-1、10-210-3,4。、[12]。:10-110-331mL10mL,3,28℃10d,Griess,,MPN。:,,30℃7d,,[13,14]。2　2.1　12℃。1。。NaHCO3NaOHpH,pHpH,。。1d,,。,。,,。1d,2d5d,。,,。,。,7d。,,。7d,,,。,。。1　Table1　Chemicalfeaturesofexperimentalteamandcontrolledteam/dpH/mg·L-1/mg·L-1/mg·L-1/mg·L-1COD/mg·L-1BOD/mg·L-1/mg·L-117.397.400.0220.0110.0230.0540.0260.0239.809.863.35—2.56—637527.477.640.1271.8430.0190.0300.9140.0239.889.693.23—3.54—747837.537.570.1712.3280.0320.0161.7610.0229.169.363.98—2.97—477547.377.510.2333.3040.0360.0262.9550.0358.838.666.00—3.20—648157.247.410.8014.4050.0460.0753.2900.0528.507.405.46—3.24—738266.897.221.4085.1930.0650.0274.8390.0528.027.216.58—3.96—609976.417.312.5267.6770.1500.0426.4590.0757.936.798.11—3.51—7411386.85—3.447—0.071—12.111—7.71—8.69—3.94—74—96.51—4.226—0.077—11.984—7.83—8.54—3.81—76—106.52—5.121—0.234—12.965—8.33—8.64—3.60—85—117.26—4.831—0.118—16.218—7.25—9.00—3.78—82—127.05—3.640—0.163—19.256—7.10—8.98—3.92—84—137.12—1.733—0.160—18.859—7.31—9.53—3.62—98—147.02—0.684—0.135—17.402—6.71—9.51—3.84—106—157.28—0.241—0.095—14.079—6.84—9.06—3.75—106—　　,。pH、。1pHpH。1d10d,11d。11d。1d12d,7d10d,133　8:,13d。1d12d,8d,。13d。。COD113d,,14d。BOD1d3d,3.0～4.0。,,,。2.2　,。、,。。,、[15,16]。2,、。1.13×106～1.85×106·g-1;1.26×105～1.70×105·g-1;2.01×103～3.42×103·g-1。,。,、。2　Table2　Changesofamountofmicrobes/-/106·g-1/105·g-1/103·g-1/104·g-1/103·g-1/102·g-102-181.131.702.010.0430.20—02-211.251.312.500.0750.75—02-241.551.262.740.240.93—02-271.601.342.880.461.500.3603-011.471.522.131.104.602.0003-031.851.363.421.104.607.502.3　1)1,,0.043×104～0.46×104·g-1。NH+-N,,。11d,,13d1.10×104·g-1,,。,,NH+4-N,,。,。,,,。2)NH+4-NNO-2-NNO-3-N。2,,NO-2-N,,0.20×103～1.50×103·g-1,。13d,,4.60×103·g-1。,。2.4　3,7d,,图1　亚硝化细菌数量变化Fig.1　Changesoftheamountofnitrosobacteria图2　硝化细菌数量变化Fig.2　Changesoftheamountofnitrobacteria1342005　,NO-2-NNO-3-N,;,,,,。,,。,,10d0.26×102·g-115d7.50×102·g-1,。图3　反硝化细菌数量变化Fig.3　Changesoftheamountofdenitrifyingbacteria2.5　pH1,223～27(6～10d),pH,pH。pH,126d10d,pH7,11d,。pH。2.6　——。,,,“”。。5～35℃,25～30℃。20℃,18d[17]。12℃,128,31,31d。,12℃,——,。——。,,。,。、,,[18,19]。2.7　、、,、,。、(synergism),,。,。,,,。3　　1),、,—12℃,,,。2),。12℃,31d。,pH7.0。。,。[　　　][1]　,,,.[J].(),2003,22(1):35-39.[2]　　,.[J].,2002,3:26-28.[3]　　,.[J].,2002,3(1):65-68.[4]　　,,,.[M].:,2004:202-206.[5]　RuizaG,JeisonbD,ChamyR.Nitrificationwithhighnitriteaccumulationforthetreatmentofwastewaterwithhighammoniaconcentration[J].WaterResearch,2003,37:1371-1377.[6]　ChenS,JuawC,ChengS.Nitrificationanddenitrificationofhighstrengthammoniumandnitritewastewaterwithbiofilmreactors[J].WaterScienceTechnology,1991,23(7):1417-1425.[7]　Sung-KooKim,InsooKong,Byung-HunLee,etal.Removalofammonium-Nfromarecirculationaquaculturalsystemusinganimmobilizednitrifier[J].AquaculturalEngineering,2000,21:139-150.135　8:[8]　.[M].:,2003:226-255.[9]　YooH,AhnK,LeeH,etal.Nitrogenremovalfromsyntheticwastewaterbysimultaneousnitrificationanddenitrification(SND)vianitriteinanintermittentlyaeratedreactor[J].WaterResearch,1999,33(1):146-154.[10]　.[J].,2004,4:26-28.[11]　　.[S].:,2002.[12]　　,,.[M].:,2002.[13]　,,.[J].,1996,15(3):18-20.[14]　,,　,.[J].,2001,8(1):33-36.[15]　,　,.[J].,2003,25(4):26-28.[16]　,,.[M].:,2002.[17]　,,.[J].,2003,19(13):58-59.[18]　,,.[J].,2003,31(2):33-35.[19]　　,,.[M].:,2004.PreliminarystudyonammonianitrogentreatmentofmicroorganisminindustrializedculturewateratcoldtemperatureChenZhongxiang,CaoGuangbin※,LiuYong,JiangShuyi,HanShicheng(HeilongjiangRiverFisheryResearchInstituteofChineseAcademyofFisherySciences,Harbin150070,China)Abstract:Tostudythemicroorganismfortreatingammonianitrogeninindustrializedculturewateratcoldtemperature,platecountmethodandmostprobablenumberwereappliedforquantitativedetectionofmicroorganismofthebiologicalbeadfilterfortreatingammonianitrogeninarecycleculturesystem.Atthesametime,physicalandchemicalfeaturesofexperimentalteamandcontrolledteamweremeasured.Theresultshowedthattheamountsofnitrobacteriaandnitrosobacteriaincreasedcontinuouslyandtheyreachedastablelevelatanaphase.Therewerenodenitrifyingbacteriaatthebeginningoftheexperiment;itsamountbegantoincreaseatanaphase.Comparingtheconcentrationsofammonianitrogen,nitrateandn