低温条件下低溶解氧污泥微膨胀的发生及分子生态学解析高春娣

37720117JOURNALOFBEIJINGUNIVERSITYOFTECHNOLOGYVol．37No．7Jul．2011100124SBRSVI150～200mL/g．FISHMPA223．CODPO34-P－80%98%NH+4-NTN52%28%SND15%SND．X703.1A0254－0037201107－1085－052009-05-31．KM200910005003PYZZ090429002183．1973—．、1-3．———4．、、5、．．SBR．11.1SBR1．7L/anoxic/oxic1.5h2.5h0.2kg/kg·d50%．ρDO13±1℃．ρMLSS3000mg/L．1pH=7.0～7.8．20111Table1Wastewatercharacteristicmg/LρCODρNH+4-NρNO－3-NρNO－2-NρPO3－4-P160～28058～780.13～1.10.04～0.244.0～6.81.21．2．3．4．5．6．1SBRFig．1SchematicdiagramoftheSBRρCOD、ρPO3－4-P、ρNH+4-N、ρNO－3-N、ρNO－2-N、ρMLSS、SVIρTNρNH+4-N、ρNO－3-NρNO－2-N．ρDOWTWMulti340i．OLYMPUS2BX51Eikelboom6．1.3FISHfluorescentinsituhybridizationFISH、、7．FISHCy3MPA223OLYMPUSDP71．22.12400Fig．2MorphologicalcharacteristicsofsludgeflocundernormalDO400timesSBRρDO2mg/LSVI90～110mL/g、2．ρDO2.0mg/L0.5mg/LSVIρDO5d166mL/g3．37dSVI167～210mL/g4．0.5hSBRSS．SBR．680173400Fig．3MorphologicalcharacteristicsofsludgeflocunderlowDO400times4SVIFig．4FluctuationofSVIduringtheexperiment2.2．、．Eikelboommicrothrixparvicella5．MPA223FISHMPA2236．51000Fig．5ThepicturesoflimitedfilamentousbulkingsludgebyGramstaining1000times6MicrothrixparvicellaMPA223FISH1000Fig．6ThepicturesofMicrothrixparvicellaMPA223inlimitedfilamentousbulkingsludgebyFISH1000times640dSVI．200～78012011500μm“”“”．．A/OA2/O．．0.5h8、．2.37Fig．7TheeffectoflowDOtotheremovalrateofpollutants7ρDOSBRCOD、PO3－4-P、NH+4-NTN．ρDOCODρDOCOD．ChudobaKSρDOCOD．ρDOPO3－4-P98%ρPO3－4-P0．ρDOρNO－xNO－x．45d52%30%．．SND15%SND．22℃10～15℃SND．31SBR13±1℃ρDO0.5mg/L0.2kg/kg·dSVI160～210mL/g．2FISHMPA223200～500μm“”SVI．3、COD、PO3－4-P80%98%．NH+4-NTN52%28%15%SND．88017．1MARTINSAPAGILLAKHEIJNENJJetal．Filamentousbulkingsludge-acriticalreviewJ．WaterRes200438793-817．2EIKELBOOMDH．ProcesscontrolofactivatedsludgeplantsbymicroscopicinvestigationM．LondonUKIWAPublishing2000．3．M．1992．4．、J．200829123342-3347．PENGYong-zhenGUOJian-huaWANGShu-yingetal．EnergysavingachievedbylimitedfilamentousbulkingunderlowdissolvedoxygenderivationoriginalityandtheoreticalbasisJ．EnvironmentalSicence200829123342-3347．inChinese5SEZGINMJENKINSDPARKERDS．AunifiedtheoryoffilamentousactivatedsludgebulkingJ．WatPollControl197850362-368．6EIKELBOOMDH．FilamentousorganismsobservedinactivatedsludgeJ．WaterRes19759365-388．7．MBRJ．200425656-58．SUNYu-jiaoWANGYongHUANGXia．MoleculeecologyanalysisoffilamentousbacteriumsludgebulkinginMBRJ．EnvironmentalSicence200425656-58．inChinese．8．J．200134279-82．CUIHong-shengBAIXiao-huiLIGangetal．SludgebulkinganditscontrolincoldregionJ．JournalofHarbinUniversityofCE＆Architecture200134279-82．inChineseInitiationandMoleculeEcologyAnalysisofLowDissolvedOxygenLimitedFilamentousBulkingatLowTemperatureGAOChun-diWULian-juHAOKunLILeiYUANJin-pingCollegeofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractActualdomesticsewagewastreatedbySBRprocess．LimitedfilamentousbulkingwasinducedbylowdissolvedoxygenatlowtemperatureandtheSVIweremaintained150－200mL/g．TheoccurrenceoflimitedfilamentousbulkingatlowtemperatureandthegrowthofmicroorganismwereresearchedthetechniqueofFISHwasadoptedtoidentifythespeciesofthedominantfilamentousbacteriainthestateoflimitedfilamentousbulkingandtheresultshowedthatthefilamentousbacteriathatledtolimitedfilamentousbulkingwasmicrothrixparvicellaMPA223．ComparedtothestatusatnormaldissolvedoxygentheremovalrateofCODandPO3－4-Pincreasedto80%and90%whiletheremovalrateofNH+4-NandTNdecreasedto52%and28%respectivelyduringlimitedfilamentousbulking．Andthedataalsoshowedthatlowdissolvedoxygenledtothephenomenonofsimultaneousnitration-denitrationSNDabout15%ofthenitrogenwasremovedbySND．Keywordslowdissolvedoxygenlimitedfilamentousbulkinglowtemperaturemicrothrixparvicella9801