电子受体亚硝酸氮在反硝化除磷过程中的作用黄荣新

27720077　　　　　　ActaScientiaeCircumstantiaeVol.27,No.7Jul.,2007:(No.GA01C201-03)SupportedbytheScienceandTechnologyProjectItemofHeilongjiangProvince(No.GA01C201-03):(1980—),,,E-mail:[e-mail];＊()Biography:HUANGRongxin(1980—),male,Ph.Dcandidate,E-mail:[e-mail];＊Correspondingauthor,,,.2007.[J].,27(7):1141-1144HuangRX,LiD,ZhangJ,etal.2007.Theeffectofnitriteaselectronacceptoronthedenitrifyingphosphorusremovalprocess[J].ActaScientiaeCircumstantiae,27(7):1141-1144黄荣新1,李冬2,张杰1,＊,李相昆1,姜安玺1,马立1,鲍林林11.哈尔滨工业大学市政环境工程学院,哈尔滨1500902.北京工业大学建筑工程学院,北京100022:2006-07-31　　　:2007-03-20:,,.,30mg·L-1,,;25mg·L-1,,、,.:;;;:0253-2468(2007)07-1141-04　　　:X703　　　:ATheeffectofnitriteaselectronacceptoronthedenitrifyingphosphorusremovalprocess　HUANGRongxin1,LIDong2,ZHANGJie1,＊,LIXiangkun1,JIANGAnxi1,MALi1,BaoLinlin11.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin1500902.SchoolofArchitectureandCivilEngineering,BeijingUniversityofTechnology,Beijing100022Received31July2006;　　　accepted20March2007Abstract:Theelectronacceptor-nitritecanbecomeinhibitoryortoxictowardsthedenitrifyingphosphorusremovalprocess.Tostudytheinhibitoryeffectofnitriteduringtheanoxicphaseofdenitrifyingphosphorusremoval,jartestswereconductedwithnitriteastheelectronacceptor.Theresultsindicatedthatmorethan30mg·L-1nitritewillseverelyinhibitanoxicphosphateuptake,butthethresholdinhibitoryconcentrationwasdependentonthecharacteristicsoftheactivatedsludgeandtheoperatingconditions.Exposuretolessthantheinhibitoryconcentrationwasnotdetrimentaltoanoxicphosphorusuptake,rathernitriteaswellasnitratereplacedoxygenastheelectronacceptors,andhadapositiveeffectondenitrifyingbiologicalphosphorusremoval.Keywords:denitrifyingphosphorusremovalbacteria;electronacceptor;denitrifyingbiologicalnutrientremovalprocess;inhibitoryconcentration1　(Introduction)2060,(Shimuraetal.,1994).(,1998;NGetal.,2001).,(Jensetal.,1999),(,).,,.,NO-x(Huetal.,2003).,,(Kerrnetal.,1993;1994).,COD()DOI:10.13671/j.hjkxxb.2007.07.011　　　　　　　　　　27、(Kubaetal.,1993;1996).,(denitrifyingphosphorusremovalbacteria,DPB)(Wanneretal.,1992;Vlekke,1998).,(Pokethitiyooketal.,1990).(Jens,1999),4～5mg·L-1,8mg·L-1.,,,.2(Materialsandmethods)2.1　材料与设备.()4L.,(KH2PO4K2HPO4)、()(5L1mL).1.1　Table1　Compositionofthenutrientsolution1L/gFeCl3·6H2O1.50H3BO30.15CuSO4·5H2O0.03KI0.03MnCl2·4H2O0.12NaMoO4·2H2O0.06ZnSO4·7H2O0.12COCl2·6H2O0.15:,COD300～400mg·L-1,K2HPO4(KH2PO4),1L5mL.:,DPB;,,,,.K2HPO4(KH2PO4),30mg·L-1,(1mL/5L).2.2　实验过程2.2.1　厌氧释磷烧杯试验　.2000mL554L,(),.2h.2.2.2　缺氧亚硝酸氮抑制的烧杯试验　,2L,30mg·L-1,MLSS3000～3500mg·L-1,COD.SRT10d,520、25、30、35、40mL(5mg·mL-1),.240min,.2.3　分析项目与方法4L,7h(2h,0.5h,4h,0.5h).2L,2L.(5mg·mL-1),.,.,.2.2　Table2　ParametersandmethodsofmeasuringwaterqualityCODTPPO3-4-PNO-2-NN—1-MLSSpHpH11427:3　(Results)3.1　在反硝化除磷中亚硝酸氮抑制浓度的确定(5mg·mL-1),、.,,1.1　TPFig.1　TPcomcentrationsatdifferentnitriteconcentrations1:20、25mL,,20mL;2,,,“”.30、35、40mL,,,“”,;:DPB,DPB;,90min,40mL,240min.:30mL、35mL、40mL,TP,,30、90120min,330.23、34.87、38.75mg·L-1.,30mg·L-1,,DPB,.3.2　间隔性投加亚硝酸氮对缺氧吸磷的积极作用(),.30mg·L-1,3,15mL5mg·mL-1NO-2-N,90min10mLNO-2-N(15mg·mL-1),240min5mLNO-2-N,300min,.MLSS3000mg·L-1,27.35mg·L-1,TPNO-2-N2,()3.2　TPNO-2-NFig.2　TPNO-2-Novertimewithnitritesolutionsaddedatintervals2,(),.23,,15mL,NO-2-N,30min,3.9781mg·g-1·h-1,,90minNO-2-N.10mLNO-2-N,3,2.1585mg·g-1·h-1,240min;2“”,1143　　　　　　　　　　273　Fig.3　Themaximumphosphorusuptakerateundereachintervaladditionphase.5mLNO-2-N,DPBNO-2-N,1.6321mg·g-1·h-1.4　(Conclusions)1),,,,.2)30mg·L-1,,;,,.3)25mg·L-1,,,.:张杰(1938—),教授,博士生导师,中国工程院院士,建设部科技委员会委员.研究方向:水污染控制与水环境恢复.References:HuJY,OngSL,NgWJ,etal.2003.Anewmethodforcharacterizingdenitrifyingphosphorusremovalbacteriabyusingthreedifferenttypesofelectronacceptors[J].WaterRec,27:3463—3471JensMeinhold,EvaArnold,StevenIsaacs.1999.Effectofnitriteonanoxicphosphateuptakeinbiologicalphosphorusremovalactivatedsludge[J].WaterRes,33(8):1871—1883Kerrn-JespersenJP,HenzeM,StrubeR.1994.Biologicalphosphorusreleaseanduptakeunderalternatinganaerobicandanoxicconditionsinafixed-filmreactor[J].WaterRes,28:1253—1255Kerrn-JespersenJP,HenzeM.1993.Biologicalphosphorusuptakeunderanoxicandanaerobicconditions[J].WaterRes,27:617—624KubaT,SmoldersGJF,vanLoosdrenchtMCM,etal.1993.Biologicalphosphorusremovalfromwatewaterbyanaerobic-anoxicsequencingbatchreactor[J].WaterSciTech,27:241—252KubaT,MurnleitnerE,vanLoosdrenchtMCM,etal.1996.Ametabolicmodelforbiologicalphosphorusremovalbydeitrifyingorganisms[J].BiotechnolBioeng,52:685—695NgWJ,OngSL,HuJY.2001.Denitrifyingphosphorusremovalbyanserobic/anoxicsequencingbatchreactor[J].WaterSciTech,42(3):139—146PokethitiyookP,McClintockSA,RandallCW.1990.Theroleofnitrateinbiologicalphosphorusremoval.EnvironmentalEngineering[A].//Proceedingsofthe1900SpecialityConference[C].Arlington,Virginia,330—336ShimuraM,TabuchaT.1999.Theeffectoflivestockontheconcentrationofnitrogeninstreamwater[J].WatSciTech,30(7):167—170VlekkeGJFM,ComeauY,OldhamWK.1988.Biologicalphosphateremovalfromwastewaterwithoxygenornitrateinsequencingbatchreactors[J].EnvironTechn