短程硝化工艺出水水质及微生物区系分析王永华

32*450001PCR-DGGE。50~400mg/L10mg/L300mg/L90%。DGGE50mg/L400mg/L0.24。X703.1A1003-6504(2009)07-0094-04AnalysisofEffluentWaterQualityandMicrobialCommunitiesofShortcutNitrificationWANGYong-huaZHAOJi-hong*LIUNanSchoolofChemistryandChemicalEngineeringHenanUniversityofTechnologyZhengzhou450001ChinaAbstractShortcutnitrificationwasusedtotreathighammonianitrogenwastewaterwithanalysisofmicrobialcommunitiesbyPCR-DGGE.Resultsshowedthatthesystemcouldenduretheimpactofinfluentammonianitrogenconcentrationfrom50~400mg/Ltheeffluentammonianitrogenconcentrationwasbelow10mg/L.AlongwiththeincreaseofinfluentammonianitrogenconcentrationaccumulationratioofNO2--Nincreasedwhentheconcentrationwasupto300mg/Ltheaccumulationratiowasnearly90%.DGGEresultshowedthatdiversityofmicrobialcommunitydecreasedevidentlyasthetimewenton.Wheninfluentammonianitrogenconcentrationwas50mg/Land400mg/Lthecomparabilityofmicrobialcommunitywasonly0.24.Keywordshighammonianitrogenwastewatershortcutnitrificationdenaturinggradientgelelectrophoresismicrobialcommunity、、。NH4++1.5O2→NO2-+2H++H2O1NO2-+0.5O2→NO3-2NO3-+16C2H5OH→NO2-+13CO2+12H2O3NO2-+14C2H5OH→12N2+OH-+12CO2+14H2O4NO2-N2325%、40%[1-2]。。SBR。。-PCR-DGGE。[e-mail]*[e-mail]。EnvironmentalScience&Technology32720097Vol.32No.7Jul.20097NH42SO4NH4+-NNaHCO3Na2HPO4。1。1.24.5LSBR1h5h1h、5min50%。1.3CODK2Cr2O7[3]MLSSNH4+-NGB7479-87NO3--NGB7480-87NO2--NGB7493-87pHPHS-4A。1.41.4.1DNACTABDNA。SDSbead-beating[4]。1.4.2PCR16srDNAV3F357GCCGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGCCTACGGGAGGCAGCAGR518ATTACCGCGGCTGCTCG。Touchdown94℃2.5min94℃45s65~55℃45s72℃45s200.5℃855℃72℃5min。1.4.3DGGEDGGETheDcodeTMUniverslMutationDetectionSystem10%=37.5：130%~50%100%7mol40%PCR45μL。1×TAE20mmolTris10mmol0.5mmolEDTApH8.062℃200V5min120V7h。SYBRGreen130min210min254nm。DGGENTSYS。22.156dNH4+-N50~400mg/L28±1℃pH7.5~8.01～3。NH4+-N———50、100、150、250、300、400mg/L。110mg/L。300mg/L60mg/LMLSS2000mg/LNH4+-N50mg/LMLSS。5NH42SO41Table1RawwaterqualityCOD(mg/L)NH4+-N(mg/L)TP(mg/L)pH30040~6057.5~8953250~400mg/L。1COD120mg/LNH4+-NCODCOD。3。300mg/L90%。。NH4+-N250mg/L。[5]。2.22.2.1SBRNH4+-N50、100、150、250、400mg/L。7%4DNA23Kbp。2.2.2PCRPCRDNADNAPCR5DNA230bp。2.2.3DGGEPCRDGGE6。6NH4+-N450mg/L400mg/LNH4+-N2、3、6。7。2。120.76340.71150.24。。311mg/L、9672Table2Thecomparabilityanalyseofmicrobialcommunity1234511.0020.761.0030.480.621.0040.480.520.711.0050.240.480.670.571.0028±1℃pH7.5~8。250mg/L400mg/L0.24。3DGGE。[][1]SchmidtISliekersOSchmidMetal.Newconceptofmicrobialtreatmentprocessesforthenitrogenremovalinwastewaters[J].FEMSMicrobiologyReviews2003274481-492.[2].[J].200528S2142-144.YaoYingZhangWenMaMin.Advancesintheprocessofbiologicalnitrogenremoval[J].EnvironmentalScience＆Technology200528S2142-144.[3],,.[M].2006.XiDan-liSunYu-shengLiuXiu-ying.EnvironmentalMonitoring[M].BeijingHigherEducationPress2001.inChinese[4]RMaaritNiemiIlseHeiskanenKaisaWalleniusetal.ExtractionandpurificationofDNAinrhizospheresoilsam－plesforPCR-DGGEanalysisofbacterialconsortia[J].Jour－nalofMicrobiologicalMethods2001453155-165.[5]WangJian-longYangNing.Partialnitrificationunderlim－iteddissolvedoxygenconditions[J].ProcessBiochemistry200439101223-1229.97