短程硝化颗粒污泥SBR的快速启动与维持吴蕾

　61　11　　　　　　　　　　　　Vol.61　No.11　201011　　CIESC　Journal　　　November　2010SBR吴　蕾,彭永臻,王淑莹,马　勇(,,100124):,SBR,28℃,0.2m3·h-1,(DO)2.0mg·L-1,(SRT)15d,2min,pH,,,80(19d),。1.5～2.0mm,COD80%95%,(NO-2-N/NO-x-N)95%。FISH,AOB,17.8%,NOB0.6%。FA。:SBR;;;;:X703.1　　　　　　:A:0438-1157(2010)11-2931-07QuickstartandmaintenanceofnitrifyinggranularsludgeinSBRprocessWULei,PENGYongzhen,WANGShuying,MAYong(KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentalRecoveryEngineering,CollegeofEnvironmentalandEnergyEngineering,BeijingUniversityofTechnology,Beijing100124,China)Abstract:Inordertoexploretheoptimalconditionsofnitrifyingsludgegranulation,testwascarriedoutinasequencingbatchreactor(SBR)atoperationconditions:temperature28℃,aeration0.2m3·h-1,dissolvedoxygen(DO)2.0mg·L-1,sludgeage(SRT)15d,leadingtosettlingtimeshortento2min.Thegranulationofnitrifyingfloccularactivatedsludgewasachievedsuccessfullyafter80stablecycles(19d)byparametercontrolofammoniaoxidationprocess,byoptimizationofaerobictimeandagainstover-aeration,andaveragediameterofsludgegranularobtainedwasbetween1.5—2.0mm.TheefficiencyofCODandammoniaremovalwas80%and95%,respectively.Thenitriteaccumulationrate(NO-2-N/NO-x-N)wasover95%.Thequantitativeresultsfromfluorescenceinsituhybridization(FISH)analysisformolecularbiologyshowthatthedominantmicro-organismwasstillAOB,about17.8%oftotalbacteria,whileNOBonly0.6%.ThemainfactorsforstableperformanceofnitrificationwereFAinhibitionandreal-timecontrol.　　2010-01-04,2010-03-05。:。:(1982—),,。:(50808004);(PHR20090502);(PXM2008014204050843)。　　　　　Receiveddate:2010-01-04.Correspondingauthor:Prof.PENGYongzhen,[e-mail]:supportedbytheNationalNaturalScienceFoundationofChina(50808004),FundingProjectforAcademicHumanResourcesDevelopmentinInstitutionsofHigherLearningundertheJurisdictionofBeijingMunicipality(PHR20090502)andtheProjectofScientificResearchBaseandScientificInnovationPlatformofBeijingMunicipalEducationCommission(PXM2008014204050843).　Keywords:SBR;nitrification;rapidcultivation;granularsludge;real-timecontrol　,,,[1]。。,,,[2-6]、[7-9]、[4,10]、[11]。、,,。:①;②。、、、[12]。,SBR,SBR,:①SBR,,;②SBR、,,。NO-2-N,NO-2-N,。SBR,,、DO、SRTpH,,。1　1.1　SBRSBR,50cm,20cm,12L,10L,,,(9cm),10cm,。,,,0.2m3·h-1。,(28±0.5)℃。Multi340ipH、DOpHDO。SBR,3～4,8min,3～4h,2min,2min2min。6L,6L,6/10,10～13h,,,SRT15d。1.2　,,400mg·L-1,NH+4-N200mg·L-1,1。,,,MLSS2200mg·L-1,SVI64ml·g-1。1　Table1　CompositionsofartificialwastewaterCompositionConcentrationTracenutrientcomposition/mg·L-1ethanol0.4ml·L-1FeCl3·6H2O1500NH4Cl0.76g·L-1H3BO3150KH2PO40.04g·L-1CuSO4·5H2O30MgSO4·7H2O0.02g·L-1KI180CaCl2·2H2O0.01g·L-1MnCl2·4H2O120tracenutrient0.575ml·L-1Na2MoO4·2H2O60ZnSO4·7H2O120CoCl2·6H2O150EDTA100001.3　COD、NH+4-N、NO-3-N、NO-2-N、MLSS[13]。BX-51C24040ZOOM。,,2.5%1.5h,3,50%、70%、80%、90%100%,,,·2932·　　　　　61　1500nm,。HITACHIS-4300(Japan)。,。,,2.5、2.0、1.5、1.0mm,,,。Amann(FISH)[14]。。,,50%、80%98%3min。,46℃2h。,48℃20min。,20～25IPP6.0Software。FISH2,EUBmixEUB338、EUB338Ⅱ、EUB338Ⅲ1∶1∶1,。2　FISHTable2　16SrRNA-targetedoligonucleotideprobesProbeSpecificityProbesequence(5′-3′)FA/%EUBmixalmostallEubacteriaFITCNSO190　ammonia-oxidizingβ-ProteobacteriaCy3　55　NIT3NitrobacterCy340Nstpa662NitrospiraCy3352　2.1　SBR,MLSS2172mg·L-1,SVI64ml·g-1,NH+4-NNO-3-N1mg·L-1,。,2min,,MLSS,12(3d),MLSS1498mg·L-1,(3h),NH+4-N47.3mg·L-1,NH+4-N62%。,MLSS,SVI,NH-4-N,28,MLSS3344mg·L-1,SVI35～40ml·g-1,NH+4-N15.2mg·L-1,NH+4-N85.9%,12。1　MLSSSVIFig.1　VariationofMLSSandSVIduringcultivationofnitrifyinggranules　,36,SBRpH。pH()(),NaHCO3,,。,pH,,NO-2-NNO-3-N,,NH+4-N,NH+4-N0.59～3mg·L-1,NH+4-NNO-2-N(NAR)95%97%,2。30,,,,SVI40ml·g-1。60,,,,3(c)。,,,。80,,·2933·　11　　:SBR,,3(d)。480。0～1.0mm、1.0～1.5mm、1.5～2.0mm、2.0～2.5mm18.01%、28.89%、40.9%、4.52%,2.5mm7.66%,1.5mm。Tay[6]0.25mm,[15]0.5mm,,。FISH,AOBNOB7%0.75%,AOB。,80,,。FISH,AOB·2934·　　　　　61　,17.8%,NOB,0.6%。FISH(5),AOB,,。2.2　,。,、,。3。,COD/N=2∶1,28℃,0.2m3·h-1,DO2.0mg·L-1,pH7.0,(SRT)15d,2min,pH,80(19d)。,,,1.5～2.0mm,4。,NO-2-N95%,NO-3-N1mg·L-1。。2.3　2.3.1　pH　pH,pH。Yang[21]pH4,,7mm,pH8,,4.8mm。pH5.8～8.5,pH7.0～8.0,pH6.0,。,pH7.5。,,pH,,NH+4-N,pH。,1molNH+4-N2molH+。pH,90min,NaHCO3,。6COD、NH+4-N、NO-3-N、NO-2-NpH。、,,,pH(6B),pH7.3～7.4,,。2.3.2　FA　FAEPS,。Yang[22],FA23.5mg·L-1,。,FA。FA,,。,FA0.1～1.0mgN·L-1,NOB[18]。Chang[23]·2935·　11　　:SBR3　Table3　OperationalconditionsandformationtimeforcultivationofaerobicgranulesinreferencesRef.T/℃Aeration/DOpHSubstrateSeedsludgeSettlingtimeSettingFormingtime/d[16]302L·min-1,DO1.5mg·L-17.5inorganic　wastewatermethanogenicand　nitritetypenitrifying　granule—pilotscaleairlift　typereactor30[17]25—7.0sodiumacetateanaerobicgranular　sludge10—5minSBR60[18]20—253.5L·min-1,1.8—5.3mg·L-17.0—7.6inorganic　wastewaternitrifyingfloccular　sludge4minsequencingbatch　airliftreactor50[19]—0.036—0.93L·(min·Lbed)-1NaHCO3additioninorganic　wastewaternitrifyingfloccular　sludge—aerobicupflow　fluidizedbed64[20]250.1m3·h-17.0—8.0domestic　wastewaternitrifyingfloccular　sludge8—4minSBR33thisstudy282.1—4.0mg·L-17.0—7.5organic　wastewaternitrifyingfloccular　sludge2minSBR19,FA0.2mgN·L-1,。6/10,,6,NOB,NOB。200mg·L-1,,110～120mg·L-1,FA1.1～1.8mgN·L-1。FANO-2-NNO-3-N。6　pH