对氯代苯胺PCA在填充床生物反应器中的降解作用

(PCA)*万登榜惠阳陈云霞丘昌强(,430072)(PCA),(pDCB)PCA.PCA.,PCALogisticq=qmax/(1+e-Uv).,Ks()qmax().PCA,.3h,PCA360mgL-1,125mgL-1h-1;91%.Degradationofpchloroaniline(PCA)inpackedbedbioreactor.WanDengbang,HuiYang,ChenYunxiaandQiuChangqiang(InstituteofHydrobiology,AcademiaSinica,Wuhan430072).Chin.J.Appl.Ecol.,1998,9(3):313~317.Withpchloroaniline(PCA)asaselectedsubstrate,themixedmicroorganismsassimilatingitasthesolesourceofcarbonandnitrogenwereobtainedbyacclimationtechniquefromtheenrichedculturalproductsofdegradedpdichlorobenzen(pDCB).ThemixedmicroorganismsimmobilizedinapackedbedreactorwereusedtodegradePCA.Inthepackedbedbioreactor,thebiodegradationofPCAfollowedtheLogisticequation,q=qmax/(1+e-Uv).Themajorkineticparameters,Ks(halfvelocityconstant)andqmax(maximumspecificsubstratedegradationrate),werecalculated.PCAwasfoundtobedegradedwithaconcomitantreleaseofchlorideionsintothesubstrate.Undertheconditionsofresidencetimeof3hoursandinletstreamatPCAconcentrationof360mgL-1,thevolumetricdegradationrateofthesubstratewas125mgL-1h-1,withremovalefficiencyof91%.KeywordsPackedbedbioreactor,Pchloroaniline,Microbialdegradation.*(92J74).1996-07-20,1998-02-18.1((PCA)),.,,..,PCA.PCA,..Surovtseva[8](20mgL-1)3,4.Zeyer[9]Moraxellasp.PCA..1998693CHINESEJOURNALOFAPPLIEDECOLOGY,Jun.1998,9(3)313~317PCA,Lattorre[5]PCA.(pDCB),PCA.PCA,,,.22.164mm,380mm,1.2~5mm.400g.,.30..498mlmin-1.525ml.1Fig.1Schematicdiagramforpackedbedreactor.2.22.2.1,,.(gL-1):K2HPO41.0;KH2PO41.0;MgSO47H200.41;CaCO30.01;FeSO47H2O0.05;1000ml;pH7.2.,1530min.2.2.2,pDCB.100ml250ml(AALD)20mg,5ml,301,AALD,2,PCA.2,PCA.2.2.3PCA,30(120min-1)24h,,,,.,(gL-1).,(mgg-1).[3].2.3[2].2.4Logistic.,Q=Qmax/(1+e-s),,Q;Qmax;S,.SQ,Qmax,.Ks1/2Qmax,.,,3149Uv(mgL-1h-1),,SUv.33.13.1.16,,308h.;,.q,Logistic:q=61.92/(1+e2.022-0.0051s),(=-0.98447,p0.01).,Qmax61.92mgg-1h-1;Ks396.47mgL-1.2.2,163488mgL-1,QS.2(q)Fig.2Specificsubstratedegradingratesatdifferentanilineconcentrationsinabatchculture.3.1.2PCA250ml100ml,PCA,50~250mgL-1.,302..3,()PCA.,max0.23d-1,PCA82%.3PCAFig.3MicrobialgrowthandaccompanyingchlorideionsreleaseatdifferentconcentrationsofPCA.3.23.2.1.,,,PCA.301,.100mgL-1PCA.83mlh-1,120mlh-1.1,,2.9h,PCA.,90%.,.,.3.2.2(D)PCA,,.(100mgL-1),.63153:(PCA)D(4).4,,PCAD;,PCA100%.,.4(D)PCA(So=100mgL-1)Fig.4VolumetricdegradingratesandpercentremovalratesforPCAbyimmobilizedmicroorgnismsindesignedrangeofdilutionrates(D).3.2.3PCA(120mlh-1),(4.375h),PCA.PCA1.0.283mgg-1,,(Q).,(Uv)QPCALogistic.Q=133.81/(1+e4.27-0.19Uv)(P1PCA(120mlh-1,4.375h)Table1RemovalratesofPCAinthebioreactor(flowvelocityforinlet:120mlh-1;hydrolicretentiontime:4.375h)PCA(mgL-1)48.8290.93156.67187.5191.49InfluentforPCAPCA(mgL-1)24.9720.9523.8423.4927.52EffluentforPCAPCA48.8576.7684.7787.4785.63Percentremovalrates(%)0.01).Qmax133.81mgg-1h-1,Ks98.23mgL-1.PCA5.15,,5(q)(Uv)Fig.5Relationcurvebetweenspecificsubstratedegradingrates(q)andvolumetricloadings(Uv).;Uv10~30mgL-1h-1,,Uv,Q.,.PCA360mgL-1,200mlh-1(,2.63h),137mgL-1h-1,125.05mgL-1h-1,PCA91.4%,.4Kellogg[4],,.pDCB,,,,,3169,,.Monod,Logistic.,()(P0.01)[1],,.,Schmidt[7].,,PCAPCA,,.,,..,[6]..QmaxKs()..,PCA100mgL-1,PCA2;,200mgL-185%4.5h.,PCA,,,PCAQmax.,,,.,.1.1987.(OPA).,8(5):2~5.2.1989.().:.3Bisping,B.1986.GlycerolproductionbycellsofSaccharomycescerevisiaeimmobilizedinsinteredglass.Appl.Microbiol.Biotechnol.,23:174~179.4Kellog,S.T.1981.Plasmidassistedmolecularbreading:newtechniqueforenhancedbiodegradationofpersistenttoxicchemicals.Science,214(4525):1133~1135.5Lattorre,J.1984.Microbialmetabolismofchloroanilines:enhancedevolutionbynaturalgeneticexchange.Arch.Microbiol.,140:159~165.6Livingston,A.G.1991.Biodegradationof3,4dichloroanilineinafluidizedbedbioreactorandasteadystatebiofilmkineticmodel.Biotechnol.Bioeng.,38:260~272.7Schmidt,S.K.1985.Modelsforthekineticsofbiodegradationoforganiccompoundsnotsupportinggrowth.Appl.Environ.Microbiol.,50(2):323~331.8Surovtseva,E.G.1984.3,4Dichloroanilineasourceofcarbonandnitrogenforamixedcultureoforganisms.Microbiology,53(1):10~15.9Zeyer,J.1985.Microbialmineralizationofringsubstitutedanilinesthroughanorthecleavagepathway.Appl.Environ.Microbiol,50(2):447~453.3173:(PCA)