多级连续电渗析分离丝氨酸和脯氨酸袁中伟

　60　5　　　　　　　　　　　　Vol.60　No.5　20095　　CIESC　Journal　　　May　2009袁中伟,钟玉江,余立新(,,100084):;;;;:TQ028.8　　　　　　　:A:0438-1157(2009)05-1199-05SeparationofserineandprolinebymultistageelectrodialysisYUANZhongwei,ZHONGYujiang,YULixin(StateKeyLaboratoryofChemicalEngineering,DepartmentofChemicalEngineering,TsinghuaUniversity,Beijing100084,China)Abstract:Inordertoseparateaminoacidswhoseisoelectricpoints(IP)areneartoeachother(takingprolineandserineasexample),amultistageelectrodialysisprocesswasdeveloped.Intheexperiment,theinfluencesofnumberofstages,currentintensityandconcentrationofNaOHonseparationefficiencywereinvestigated.TheresultsshowedthatitwasfeasibletoseparatenearIPaminoacidsbyusingmultistageelectrodialysis.Increasingthenumberofstagescouldobviouslyimprovethepurityofprolineproduct,whilehavelittleeffectonthepurityofserineproduct,increasingcurrentintensitycouldimprovethepurityofprolineproduct,whilehavelesseffectonthepurityofserineproduct.AproperamountofNaOHinthefeedwashelpfultotheseparation.Keywords:multistageelectrodialysis;serine;proline;anionexchangemembrane;bipolarmembrane　　2008-09-03,2009-02-20。:。:(1982—),,。:(2003CB615701)。　　,。、、[1-3],、、,。Shaposhnik[4](barriereffect)。[5]、　　Receiveddate:2008-09-03.Correspondingauthor:Prof.YULixin.E-mail:[e-mail]:supportedbytheNationalBasicResearchPro-gramofChina(2003CB615701).　,,,96%[5];[6],。(,5.686.48),90%[7]。,“”(multistageelectrodialysis)。Glueckauf[8]1956。Takahashi[9]Cruz[10](Li-NaLi-K),“”。[11]DL-,,1.051.20。,,、NaOH;。1　,,、、,。,0.1mol·L-1,NaOH0、0.02、0.20mol·L,,1。1,,,。NaOH,,;NaOH,1,,NaOH,。。1,,,。,,。,NaCl,1　NaOHTable1　ConcentrationsofionsinfeedwithdifferentNaOHaddingamounts/mol·L-1Na+Ser+Ser±Ser-Pro+Pro±Pro-OH-02.64×10-51.00×10-14.33×10-51.59×10-51.00×10-16.72×10-76.13×10-90.023.78×10-88.04×10-29.98×10-22.82×10-89.96×10-23.77×10-43.45×10-60.203.00×10-141.54×10-49.98×10-29.60×10-139.03×10-39.10×10-29.19×10-31　Fig.1　Schematicdiagramofexperimentalapparatus　·1200·　　　　　60　;,[12-14]H+,。2　。AstomAM-1TokuyamaBP-1,7cm×13cm,4cm×10cm。2mm,0.85mm。1.2ml·min-1,PSA-GKX-120;25ml·min-1,ZG60-600。L-850T6。0.1mol·L-1,NaOH;400ml1%NaCl。,。3　3.1　0.1mol·L-1,NaOH0.02mol·L-1,,0.56、0.64、0.72A,。2。2,,,。,NaOH,,,。,,,。,。,3。4,,。1,1,,。3.2　5。5:,40.72A0.80A90%;。,。,,,,,。·1201·　5　　:,Ser-/Pro-Ser/Pro,Ser/Pro,,Ser-/Pro-Ser-/Pro-,,()。,,,。:0.1A,56.4%,90.7%。3.3　NaOH40.8A,,NaOH,NaOH,67。6,NaOH,63.8%55.4%,80.1%55.8%。NaOH,,,。7,NaOH,,,。,NaOH、。4　　,。,,,。,,,;,·1202·　　　　　60　。NaOH,NaOH,,NaOH,。References[1]　BaiYunfeng(),etal.Advanceofseparationandpurificationofaminoacid.FoodResearchandDevelopment(),2007,128(2):175-178[2]　WuCailian(),etal.Separationandpurificationofaminoacidsbyionexchange.AminoAcids&BioticResources(),2005,27(4):50-53[3]　WengLianjin(),etal.Separationtechniquesformixedaminoacids.ChemicalIndustryandEngineeringProgress(),2000(2):51-52[4]　ShaposhnikVA,EliseevaTV.Barriereffectduringtheelectrodialysisofampholytes.J.Mem.Sci.,1999,161:223-228[5]　WangHui(),YuLixin().Separationofmixedaminoacidsbybipolarmembraneelectrodialysis.J.TsinghuaUniv.:Sci.&Tech.(:),2004,12:1588-1591[6]　ZhongYujiang(),YuLixin().Removalofglutamicacidfromglutaminefermentationbrothbyelectrodialysis.ChemicalIndustryandEngineeringProgress(),2007(6):882-885[7]　WangHui(),YuLixin().Separationofprolineandserinebybipolarmembraneelectrodialysis.MembraneScienceandTechnology(),2005,5:21-25[8]　GlueckaufE,KittGP.Anewelectrolyticseparationtechniqueusingsemi-permeablemembranes.J.Appl.Chem.,1956,6:511-516[9]　TakahashiK,UmeharaK,etal.Mutualseparationoftwomonovalentmetalionsbymultistageelectrodialysis.Chem.Eng.Sci.,2005,60:727-734[10]　CruzGTP,GaspilloPD,etal.SelectivetransportofLi-NaandLi-Kbinarysystemsacrossacationexchangemembraneunderanelectricfield.Sep.Pur.Tech.,2000,19(1/2):21-26[11]　WangJingshi(),PengYong(),YuLixin().MultistageelectrodialysisfortheseparationofDL-phenylglycinebychiralselectivecation-exchangemembrane.MembraneScienceandTechnology(),2008,28(2):89-91[12]　XuTongwen(),SunShusheng(),etal.Thefunctionsandconstructionsofabipolartypeionexchangemembraneelectrolyticcell.MembraneScienceandTechnology(),2000,20(1):53-59[13]　XuTongwen(),WangZhiwu(),LiuNing().Therecentreviewontheprincipleandapplicationofbipolarmembrane.TechnologyofWaterTreatment(),1998,24(1):22-27[14]　TangYu(),WangXiaolin(),GongYan(),YuLixin().Theoreticalandappliedresearchonbipolarmembraneelectrodialysis.ChemicalIndustryandEngineeringProgress(),2004,23(10):1107-1112·1203·　5　　: