反硝化除磷菌驯化富集方式的探讨刘建广

:,,1964,,,。＊(No.2005GG21006001);(No.XN040105)。反硝化除磷菌驯化富集方式的探讨＊刘建广1　宋武昌2　王珊珊1(1.,　250101;2.,　250033)　　　SBR。,,(SVI)60、50mL/g,77%71%。PO3-4-P97%95%,PO3-4-P1mg/L。3.7%()。,。　　　　SBR　　Thestudyoncultureandenrichmentofdenitrifyingphosphorus-removingbacteria　LiuJianguang1,SongWuchang2,WangShanshan1.(1.SchoolofMunicipal&EnvironmentalEngineering,ShandongJianzhuUniversity,JinanShandong250101;2.JinanWaterandWastewaterMonitoringCentre,JinanShandong250033)Abstract:　Theenrichmentcultureofdenitrifyingphosphorus-removingbacteria(DPB)insequencingbatchre-actorswerestudiedbytwoprocesses:one-phasemodelandtwo-phasemodel.Theresultsdemonstratedthatthesludgesettleabilitywasbetterafteracclimationbythetwoprocesses,showingSVIof60mL/gand50mL/g,andtheRDPBreached77%and71%,respectively.Phosphorusandnitrateremovalefficienciesinthetwoprocesseswereabove97%and95%,respectively.Theconcentrationofphosphorusattheendofanoxiccyclewaslowerthan1mg/L,andthephosphoruscontentinthesludgereached3.7%.Thus,theDPBwasdevelopedandfunctioningeffec-tivelyinbothprocesses.Keywords:　denitrifyingphosphorus-removingbacteria;SBR;sludgeculture;phosphoruscontentinsludge　　,[1],[2]31-32。(DPB),,[3]3971-3974,[4]。,(COD)、(PAOs)。　　,,[5-7]、[8][9],。,。,/(A/O),/(A/A)[10-12],//(A/A/O)。,。A/A/O,;A/O,A/A/O。,,,。1　1.1　试验装置SBR,SBR,300mm,20L。60r/min,,,DO2～3mg/L。SBR。·24·　　30　12　200812DOI:10.15985/j.cnki.1001-3865.2008.12.007。。1。1　Fig.1　SchematicdiagramofSBRsystem1—;2—;3—;4—;5—;6—SBR;7—;8—;9—;10—;11—1.2　试验用水与接种污泥,NH4HCO3、KH2PO4、K2HPO4。,。HClNaOHpH,NaNO3(100g/L)。1。1　Table1　CompositionofsyntheticwastewaterCOD/(mg·L-1)NH+4-N/(mg·L-1)PO3-4-P/(mg·L-1)pH200～40010～207～157.0～7.5　　,MLSS4500mg/L,MLVSS3000mg/L,MLVSS/MLSS0.667,(SV30)50%,(SVI)110mL/g。1.3　分析项目及方法2。2　Table2　AnalyticparametersandmethodspHPHS-3CpHDOHQ20LDOCOD[13]PO3-4-P[13]NH+4-N[13]NO-3-N[14]MLSS/MLVSSSV3030min2　2.1　二段式驯化2.1.1　20074,153,6.5h,0.5h,2.0h,3.0h,1.0h;162,8.5h,0.5h,4.0h,3.0h,1.0h。MLSS4000～6000mg/L,MLSS。2。2　A/OFig.2　PhosphorusremovalinA/Ophaseoftwo-phasemodel　　,,(PO3-4-P(),)(PO3-4-P()),PO3-4-P30%。,16,4.0h。2,,。41(2mg/L),7mg/L,PO3-4-P,PO3-4-P。COD50%,(PHB),。,,。,。2.1.2　1,,2.0h,22.0h。20076,2,8.5h,0.5h,2.0h,3.0h,2.0h,1.0h。,50～60mg/L,。3。　　3,,,。·25·　,(PO3-4-P(),),2mg/L17mg/L,99%。17COD,PO3-4-P26.6mg/L,PO3-4-P11.6mg/L,PO3-4-P1.6mg/L;,60%,90%。3　A/A/OFig.3　PhosphorusremovalinA/A/Ophaseoftwo-phasemodel　　,,1270,,PO3-4-P97%96%。,PO3-4-P1mg/L(27。27,PO3-4-P)。,,SV3025%,SVI50mL/g。2.2　一段式驯化,A/A/O。2,8.5h,0.5h,2.0h,3.0h,2.0h,1.0h。,50～60mg/L,。MLSS4000～6000mg/L,MLSS。4。　　4,,、(PO3-4-P())。,、,PO3-4-P。35,,PO3-4-P90%,PO3-4-P,PO3-4-P97%。97.1%,PO3-4-P。55d,。PO3-4-P98%95%,,PO3-4-P1mg/L。,,SV3032%,SVI60mL/g。4　Fig.4　Phosphorusremovalinonephase-model2.3　反硝化除磷菌比例及污泥含磷率的测定,。WACHTMEIS-TER[15]MEINHOLD[2]38-41。,。KERRN-JESPER-SEN[16]2:,,,。[17],(1)SBR。RDPB=qan/qae×100%(1):RDPB,%;qan,mg/(L·h);qae,mg/(L·h)。　　(1),。　　SBR,2.0h,1L4。1,3·26·　　30　12　200812(60r/min),50、100、150mg/L。(1)RDPB,5。5,RDPB77%71%,[18](RDPB=73%),LEE[3]3972(RDPB=64%)。。　　RDPB50mg/,,RDPB。,。5　RDPBFig.5　VariationofRDPBwithdifferentnitrateconcentration　　[19]。,、、2.3%～3.7%(),。,,,。,。3　　,,。,SBR。、、2.3%～3.7%,。:[1]　KUBAT,VANLOOSDRECHTMCM,HEIJINENJJ.Phos-phorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].Wat.Res.,1996,30(7):1702-1710.[2]　MEINHOLDJ,FILIPECDM,DAIGGERGT,etal.Charac-terizationofthedenitrifyingfractionofphosphateaccumulatingorganismsinbiologicalphosphateremoval[J].Wat.Sci.Techn-ol.,1999,39(1).[3]　LEEDS,JEONCO,PARKJM.Biologicalnitrogenremovalwithenhancedphosphateuptakeinasequencingbatchreactorusingsinglesludgesystem[J].Wat.Res.,2001,35(16).[4]　AHNJ,DAIDOUT,TSUNEDAS,etal.Characterizationofdenitrifyingphosphate-accumulatingorganismscultivatedunderdifferentelectronacceptorconditionsusingpolymerasechainreaction-denaturinggradientgelelectrophoresisassay[J].Wat.Res.,2002,36(2):403-412.[5]　,,,.ORP[J].,2004,25(4):54-58.[6]　,,.CODSBR[J].,2005,5(6):30-33.[7]　,,,.[J].,2005,6(9):20-24.[8]　.[D].:,2005.[9]　HUJY,ONGSL,NGWJ,etal.Anewmethodforcharacter-izingdenitrifyingphosphorusremovalbacteriabyusingthreedifferenttypesofelectronacceptors[J].Wat.Res.,2003,37(14):3463-3471.[10]　.[D].:,2003.[11]　,,.[J].,2006,7(5):74-77.[12]　,,.[J].,2006,22(13):105-108.[13]　《》.[M].4.:,2002.[14]　,,.[J].,1999,16(1):42.[15]　WACHTMEISTERA,KUBAT,VANLOOSDRECHTMCM,etal.Asludgecharacterizationforaerobicanddenitrifyingphosphorusremovingsludge[J].Wat.Res.,1997,31(3):471-478.[16]　KERRN-JESPERSENJP,HENZEM.Biologicalphosphorusuptakeunderanoxicandaerobiccondition[J].Wat.Res.,1993,27(4):617-624.[17]　ZENGRJ,SAUNDERSAM,YUANZG,etal.Identificationandcomparisonofaerobicandpolyphosphate-accumulatingorganisms[J].Biotech.Bioeng.,2003,83(2):140-148.[18]　,,,.[J].,2004,24(1):45-49.[19]　.[J].,2006,22(2):85-86.责任编辑:赵　多　(:2008-06-02)·27·