复合垂直流人工湿地微生物特征对典型污水的响应差异

29820168ＲesearchofEnvironmentalSciencesVol．29No．8Aug．2016．J．20162981200-1206．CHANGJunjunWUSuqingLIANGKangetal．Ｒesponsesofmicrobialfeaturesinintegratedvertical-flowconstructedwetlandsIVCWsfortreatmentoftwotypesofrepresentativewastewaterJ．ＲesearchofEnvironmentalSciences20162981200-12[e-mail]．*1971-、[e-mail]12131411*1．4300722．6500913．3300294．100049．IVCW、．IVCW、、2.8～4.6、7.9～10.3、19.3～41.74.5～10.8．IVCW1.4～5.31.3～3.9IVCW．IVCW．IVCW．IVCWX1721001-6929201608-1200-07ADOI10.13198j．issn．1001-6929．2016．08．13ＲesponsesofMicrobialFeaturesinIntegratedVertical-FlowConstructedWetlandsIVCWsforTreatmentofTwoTypesofＲepresentativeWastewaterCHANGJunjun12WUSuqing13LIANGKang14WANGFeihua1LIANGWei1*1．StateKeyLaboratoryofFreshwaterEcologyandBiotechnologyInstituteofHydrobiologyChineseAcademyofSciencesWuhan430072China2．SchoolofEcologyandEnvironmentalScienceYunnanUniversityKunming650091China3．JiangxiAcademyofEnvironmentalSciencesNanchang330029China4．UniversityofChineseAcademyofSciencesBeijing100049ChinaAbstractWastewaterqualityisoneoftheimportantinfluencingfactorsformicrobialfeaturesinconstructedwetlandsCWandmicroorganismsplayakeyroleinthepollutantremovalperformanceofCW．Twogroupsofintegratedvertical-flowconstructedwetlandsIVCWswereappliedtotreatsyntheticdomesticwastewaterDWandtailwaterTWfromasewagetreatmentplant．EnzymeactivitiesnitrificationanddenitrificationratestogetherwithmicrobialcommunitystructureintheIVCWswerecomparativelyinvestigatedtorevealtheresponsedistinctionsofmicrobialfeaturestothetwotypesofwastewater．Theresultsshowedthattheactivitiesofdehydrogenasecatalaseureaseandnitratereductaseinthedown-flowchamberfedwithDWwereallsignificantlyhigherthanthoseinotherchamberswithmeanvaluesof2.8-4.67.9-10.319.3-41.7and4.5-10.8timeshigherthantheothers．Thenitrificationratesinthedown-flowandup-flowchamberofIVCWstreatingDWwere3.8and3.0timeshigherthanthoseinthesystemfedwithTWrespectively．Nosignificantdifferencesweredetectedforthedenitrificationratesincorrespondingchamberof8IVCWstreatingthetwotypesofwastewater．Ｒelativelylessinfluenceofwastewatertypeonsignaturefattyacidsandmicrobialcommunitystructureinthedown-flowchambersofIVCWswasfoundbothwithanaerobicandgram-positivebacteriadominantlypresented．Fattyacidscompositionsinup-flowchamberswereaffectedbythewastewatertypetosomeextentandtherelativeabundanceoffungiwashigher．TheeffectofwastewatertypeonenzymeactivitiesandnitrificationratewasgreaterthanthoseondenitrificationrateandmicrobialcompositionsintheIVCWs．Keywordsintegratedvertical-flowconstructedwetlandsIVCWsenzymeactivitiesnitrificationanddenitrificationratesratiosofsignaturefattyacidsmicrobialcommunitystructure、、、、1-4．5-67-9．———、10-11．、、、11-13．．、．11.14IVCW1m×1m×1m1m×1m×1m．、45cm、10～20mm4030cm、2～10mmArundodonaxvar．versicolorCannaindica8m2．PVC．1.IVCWρTN、ρTN．2d125mmd2.5d．20115—20121．1Table1InfluentwaterqualitycharacteristicsofthedomesticwastewaterandtailwaterfromsewagetreatmentplantpH6.6～8.26.7～8.3ρTNmgL8.8～15.011.69.7～14.712.1ρDOmgL0.5～6.92.61.6～8.55.8ρNH4+-NmgL4.4～10.27.10～0.90.1μScm343～550410320～490393ρNO3－-NmgL0～2.31.18.6～13.811.8℃5.7～34.321.95.7～34.221.9ρNO2－-NmgL0～0.40.10～0.30.1ρCODCrmgL82.0～148.5108.212.0～32.519.2ρTPmgL0.8～1.51.10.8～1.61.1．1.21.2.11Orion5-starThermoFisherScientificCompanyUSAρDO、pH、EC、14ρTN、ρNH4+-N、ρNO3－-N、ρNO2－-N、ρTPρCODCrDＲ2010HachCompanyUSA．1.2.220116、8、102012110cm、－20℃．102129TTCgTTC24hTPFμgμgg·24h．KMnO4g1hH2O20.02molLKMnO4mLmLg·h．g48hNH4+-Nmgmgg·48h．g24hNO2－-Nμgμgg·24h．15．16g1hNO2－-Nμgμgg·h．17gNO3－-Nmgmgg·24h．18Agilent6890GC5973MSAgilentTechnologiesInc．USA．HP-5MS31.4m×0.25mm×0.25μm250℃0.8mLmin99.999%．80℃2min50℃min150℃2min2.5℃min195℃3min2.5℃min240℃5min．4minEI70eV150℃230℃280℃SIM．19．1.33SPSS17.0IVCW．P＜0.05．22.1IVCWIVCW2.2IVCWCODCr10—1CODCr82.3%．10—1IVCWTN．2ρDO20-21NH4+-N．5—10CNIVCWNO3－-NLIN22IVCW．2IVCWTable2EffluentwaterqualitiesandpollutantremovalperformanceoftheIVCWs5—66—88—1010—1℃25.324.729.729.522.122.810.110.2pH7.27.47.67.87.47.57.67.8ρDOmgL1.41.51.31.71.72.42.23.5μScm504448495441514452595570CODCr%86.978.488.168.687.767.982.354.4TN%34.761.946.278.656.879.733.729.2NH4+-N%23.0—26.3—9.1—13.3—NO3－-N%—62.0—78.7—81.3—30.8．2.2IVCWIVCW1．1IVCW、、2.8～4.6、7.9～10.3、19.3～41.74.5～10.8P＞0.05．．IVCW23IVCW．20218110—1IVCWCODCr．NH4+-NIVCW．411．1IVCWFig.1DifferencesofresponsesofenzymeactivitiestothetwotypesofwastewaterintheIVCWs2.3IVCWIVCW2.2IVCW1.4～5.31.3～3.9．ρNH4+-NρNH4+-N24．ρNH4+-N．ρNO3－-N25．ρNO3－-NIVCW．IVCW、P＞0.05．1．2.42.4.1C11～C2026．26-27．3021292IVCWFig.2DifferencesofresponsesofnitrificationanddenitrificationratestothetwotypesofwastewaterintheIVCWstc18∶2ω9c12∶02OH、12∶03OH、i15∶0、a15∶0、15∶0、14∶03OH、i16∶0、16∶1ω9、i17∶0、cy17∶0、17∶0、18∶1ω9c、18∶1ω9tcy19∶0i15∶0、a15∶0、i16∶0、i17∶0G+12∶02OH、12∶03OH、14∶03OH、16∶1ω9、cy17∶0、18∶1ω9c、18∶1ω9tcy19∶0G－G+G－．3．3IVCW1IVCWρDO．IVCWCOD．WU28．18∶1ω9t18∶1ω9cIVCW、．IVCWIVCW．27．3G+G－1G+．G+G－1．2.4.226．Findlay29GroupⅠGroupⅡC14～C16GroupⅢC17～C19GroupⅣ．IVCW4．30-31．4IVCW45.9%～53.4%IVCW11.0%～22.0%．．0.1%～0.3%．