复合脱氮菌群的构建及其脱氮特性研究尹明锐

28320105()JournalofBeijingTechnologyandBusinessUniversity(NaturalScienceEdition)Vol.28No.3May2010　　:1671-1513(2010)03-0055-05尹明锐,　汪　苹,　刘健楠,　王　磊,　酒卫敬(北京工商大学化学与环境工程学院,北京　100048)　:利用筛选得到的5株性能良好的异养硝化-好氧反硝化菌,采用两两组合方式,构建出了优于单一菌株脱氮活性的复合菌群F1.研究了该复合菌群的生长曲线,探讨了盐度对复合菌群F1的脱氮性能的影响.研究表明:复合菌群F1在盐度2.0%,3.5%的条件下均能生长.在盐度0,2.0%,3.5%的条件下,NH+4-N和TN去除率在48,84,96h时均达到80%以上,值得一提的是,复合菌群F1在盐度2.0%时,NH+4-N和TN去除率仍很高,均在94%以上.复合菌群F1在处理实际味精废水方面具较高应用价值.:异养硝化;好氧反硝化;复合菌群;脱氮:X172;TS208;TS264.2+3　　　　　:A:2009-11-12:“”(2007BAK36B07);“”.:(1985—),,,,;　(1953—),,,,..　　,.、、,.-.,,,;,,,,.,,.,-,、[1-5],.,,,.,-.-,,,.1　1.1　1).(NH+4-NCODCr200mg/L1500mg/L,NH+4-N15mg/L,TN75%,CODCr100mg/L).LH1-x,LH2-x.A、B(CODCr90%,TN80%),AWYLW1-x;BWYLW2-x.552).BTB(g/L)[6]:20,KNO31,KH2PO41,FeCl2·6H200.5,CaCl2·7H200.2,MgSO4·7H2O1,8.5,(BTB)(1%)1mL,1mol/LNaOHpH7.0～7.3,121℃20min,.LB(g/L):KNO31,KH2PO41,FeCl2·6H2O0.05,CaCl2·7H2O0.02,MgSO4·7H2O1,8.5,121℃20min,.DM(g/L):KNO30.72,KH2PO41,MgSO4·7H2O1,2.8,121℃20min,.(g/L):(NH4)2SO40.47,KH2PO41.0,FeCl2·6H2O0.5,CaCl2·7H2O0.2,MgSO4·7H2O1.0,4.08,121℃20min,.(g/L):(NH4)2SO40.47,5.62,50mL,121℃20min,.(g):K2HPO45.0,FeSO4·7H2O0.05,NaCl2.5,MgSO4·7H2O2.5,MnSO4·4H2O0.05,1L.1.2　1.2.1　好氧反硝化菌株的筛选,0.1mLBTB,,30℃2～3d,,,.,4℃.DM,.NO-3-N60%,.1.2.2　异养硝化菌株的筛选1.2.1NO-3-N60%,30℃、160r/min(DO5～7mg/L),5%()200mL250mL,30℃、160r/min,.NH+4-N、NO-3-N、NO-2-N,NH+4-NTN(NH+4-N+NO-3-N+NO-2-N),-.(8000r/min),.NH+4-N60%,.,-.1.2.3　异养硝化-好氧反硝化复合脱氮菌群的构建-,30℃、160r/min(DO5～7mg/L)24h.、,,2.5%()200mL250mL,30℃、160r/min,.,,NH+4-N、NO-3-N、NO-2-N、CODOD600,、,1.1　-Tab.1　Multipleheterotrophicnitrification-aerobicdenitrificationmicroorganismsF1WYLW1-10+WYLW2-4F2WYLW1-10+LH1-1F3WYLW1-10+LH2-1F4WYLW1-10+LH2-3F5WYLW2-4+LH1-1F6WYLW2-4+LH2-1F7WYLW2-4+LH2-3F8LH1-1+LH2-1F9LH1-1+LH2-3F10LH2-1+LH2-31.2.4　高效脱氮菌群的耐盐性研究1.2.32%,3.5%().30℃、160r/min.NH+4-N、NO-3-N、NO-2-N、CODOD600.1.3　NH+4-N:[7];NO-2-N:N-(1-)-[7];NO-3-N:[7];,721600nm,OD600;COD:5B-1COD.2　2.1　-1.2.1,2556()　　　　　　　　　　　　　20105,WYLW1-1～WYLW1-10,WYLW2-1～WYLW2-9,LH1-1～LH1-3,LH2-1～LH2-3.,60%15,1.2.2,10,5,WYLW1-10、WYLW2-4、LH1-1、LH2-1、LH2-3,2.2　-Tab.2　Performanceofoptimizedheterotrophicnitrification-aerobicdenitrificationstrains%NO-3-NTNNH+4-NTNCODWYLW1-1065.3864.2965.3865.0478.95WYLW2-462.6561.5372.1370.1682.23LH1-192.7091.5192.7090.6377.32LH2-188.8787.6370.5669.3566.89LH2-397.3095.9897.3095.4489.82　　:、96h.2.2　-5-,3.3,F1、F4、F639hTN95.18%,92.54%,92.16%.F2、F5、F739h,TN90%,48hTN96.58%,95.23%,94.17%.23:F1,96h39h,(TN),78.95%82.23%95.18%.F1、F2、F4、3　-Tab.3　Performanceofmultipleheterotrophicnitrification-aero-bicdenitrificationmicroorganisms%NH+4-NTNCOD/hF196.2095.1869.7140F297.2496.5880.6448F340.8534.8445.9248F494.2092.5471.4840F597.5295.2367.9648F693.1892.1668.3140F796.3994.1773.6048F859.2656.3645.3748F977.9276.9560.3948F1068.3565.2450.2948　　:NH+4-N,NH+4-N、NO-3-NNO-2-N,TN=NH+4-N+NO-3-N+NO-2-N.F5、F6、F7,,,,,,.,,,.EM(effectivemicroorganisms),,,,,,,.-WYLW1-10、WYLW2-4,F1WYLW1-10、WYLW2-4,.,、,F1.2.3　F12.3.1　复合菌群F1的生长曲线F1,OD600NH+4-N,F1,1.1,F1,2h,,OD600,,2～40h,.,,,,,40h.57283　　　　　　　　　　:1　F1Fig.1　GrowthcurveandcharacteristicsofmultiplemicroorganismsF11,40h,F1,NH+4-N3.15mg/L,NH+4-N,96.06%,NH+4-N,48hNH+4-N6.28mg/L,OD600,,.2.3.2　复合菌群F1的耐盐性研究,1%～3.5%,F1,.,F12%,3.5%.2NH+4-N.2,F10,2.0%,3.5%,48,84,96h,NH+4-N6mg/L.NH+4-NTN,3.3,NH+4-NTN48,84,96h80%.,F12%,NH+4-NTN,94%.:,.,.[8],,NH+4-NNO-2-N　NO-3-N,4,NO-3-NNarNO-2-NNirNONorN2ONosN2(5,3).,,,.,88%,,F1.,.2　NH+4-N-Fig.2　Ammonianitrogenconcentration-timevariationsfigureinconditionofdifferentsalinity3　NH+4-NTNFig.3　Ammonianitrogenandtotalnitrogenremovalofdifferentsalinity3　　1)-10,,5-WYLW1-10、WYLW2-4、LH1-1、LH2-1、LH2-3,596h60%,.2)WYLW1-10、WYLW2-4、LH1-1、LH2-1、LH2-35,,10,F1、F4、F6,39hTN95.18%,92.54%,92.16%.58()　　　　　　　　　　　　　20105,,,.3)F1,F12.0%,3.5%.0,2.0%,3.5%,NH+4-NTN48,84,96h80%,,F12.0%,NH+4-NTN,94%.,F1.:[1]　,,,.[J].,2007(1):11-13.[2]　,.-[J].,2008,21(3):121-125.[3]　,,,.[J].,2009,29(1):47-52.[4]　,,,.[J].,2009,34(1):6-9.[5]　KimYJ,YoshizawaM,TakenakaS,etal.IsolationandcultureconditionsofaKlebsiellapneumoniaestrainthatcanutilizeammoniumandnitrateionssimultaneouslywithcontrolledironandmolybdateionconcentrations[J].BiosciBiotechnolBiochem,2002,66(5):996-1001.[6]　TakayaN,MariaAntoninaBCS,YasushiS,etal.Aer-obicdenitrificationbacteriathatproducelowlevelsofni-trousoxide[J].AppandEnvirMicrobio,2003,69(6):3152-3157.[7]　《》.[M].4.:,2003.[8]　Hung-SooJoo,MitsuyoHirai,MakotoShoda.Character-isticsofammoniumremovalbyheterotrophicnitrification-aerobicdenitrificationbyalcaligenesfaecalisNo.4[J].JournalofBioscienceandBioengineering,2005,100(2):184-191.STUDYONCONSTRUCTIONANDCHARACTERISTICSOFMULTIPLEHETEROTROPHICNITRIFICATION-AEROBICDENITRIFICATIONMICROORGANISMSYINMing-rui,　WANGPing,　LIUJian-nan,　WANGLei,　JIUWei-jing(CollegeofChemicalandEnvironmentalEngineering,BeijingTechnologyandBusinessUniversity,Beijing100048,China)Abstract:MultiplemicroorganismsF1withhigherefficiencywereconstructedwithfivestrainswhichhadgoodperformanceofheterotrophicnitrification-aerobicdenitrification.Thecharacteristicsofmultiplemi-croorganismsF1builtbytwo-twocombinationsisbetterthanasinglestrain.