复合型微生物絮凝剂产生菌生长周期中参数变化杜宝山

　45　1()Vo.l45　No.1　20071JOURNALOFJILINUNIVERSITY(SCIENCEEDITION)Jan　20071,2,1,1,　3(1.,130061;2.,028000;3.,130021):研究复合型微生物絮凝剂产生菌的生长曲线变化在不同时期的pH值、基质-葡萄糖、硫酸铵含量、絮凝活性、产量变化、细胞絮凝效果变化等.结果表明,在整个生长周期中,pH值基本不变;碳氮源变化显著,并存在相关性;进入稳定期后,60h时,絮凝活性达到最大值97.35%,此后,絮凝活性数值下降,但不明显,絮凝剂产量最大值为3.02g/L;细胞絮凝效果与细胞生长呈正相关性.鉴定结果表明,3种单菌分别为Brevibacteriummcbrellneri,Burkholderiaglumae,Cryptococcusalbidusvaraerius.:复合型微生物;絮凝剂产生菌;生长周期;参数变化:X172　　:A　　:1671-5489(2007)01-0138-05ParameterChangeinGrowthCycleofCompoundBioflocculantProductBacteriumDUBao-shan1,2,ZHANGYu-ling1,ZHANGLan-ying1,YAOJun3(1.CollegeofEnvironmentandResources,JilinUniversity,Changchun130061,China;2.CareerCollegeinTongliao,Tongliao028000,InnerMongoliaAutonomousRegion,China;3.Dredging-projectCo.Ltd.,Changchun130021,China)Abstract:Thechangesinthecontentofbothsubstrate-glucoseandammoniumsulfate,pHandflocculationactivityandoutputofcompoundmicrobialflocculantindifferentperiodsofgrowthcyclecompoundbioflocculantproducing-bacteriumwerestudied.Inthewholegrowthcycle,pHwasstable,thechangeofcarbonandnitrogensourcewasobvious,thecontentofnitrogenwasconsistentwiththesourceofcarbon;themaximumofflocculation-activityreachedto97.35%at60hpointaftersteadyperiod,thenthenumericalvalueofflocculationactivityfelldown,butnotobvious.Flocculationactivityhadawideregionoflargestvalueandthemaximumoutputofflocculation(3.02g/L)wasinthisregion.Theresultofcellflocculationandgrowthofcellgotpositivepertinence.Appraisalresultis:3bacteriaarerespectivelyBrevibacteriummcbrellneri,BurkholderiaglumaeandCryptococcusalbidusvaraerius.Theexperimentalresultofferedafoundationtofurtherinvestigationsofcontributingfactoronflocculationandmechanism.Itprovidedgistintheoryforcultureinindustrialapplication,too.Keywords:compoundmicrobial;flocculationproduct-bacterium;growthcycle;changeofparameter:2006-03-10.:(1963～),,,,,.:(1973～),,,,,E-mall:[e-mail].:973(:2004CB418505)(:419070200045).,,[1～3],DOI:牨牥牨牫牬牨牫牤jcnkijdxblxb牪牥牥牱牥牨牥牪牴.[4～6],.、、,3,21.,、、、、,,、.1　1.1　1.1.1　复合型微生物絮凝剂产生菌的最佳培养条件　(g/L):2.0g;K2HPO43H2O5.0g;KH2PO41.0g;MgSO47H2O0.20g;(NH4)2SO47H2O0.20g;1L,pH=7.:0.06MPa,20～30℃,100～140r/min.1.1.2　复合型微生物絮凝剂产生菌生长周期中各参数测试所用试液提取步骤　1000mL,500mL、1.0mL,120r/min、30℃,,OD660nm、pH,(6000r/min,10min),,、、、.1.2　:VIS-723G,660nm,.pHpH.[7].[8].:250mL,0.5h,(6000r/min),,PBS,,40℃,,.:250mL,pH=7.05.0g/L50mL,1%CaCl21.00mL,0.50mL,,pH6.0～7.03min,2min,752OD550nm,OD550nm,OD550nm:(%)=(A-B)/A×100%,AOD550nm;BOD550nm.250mL,pH=7.05.0g/L50mL,1%CaCl21.00mL,0.50mL(),.1.3　、BiologMicrostation.250mL,30℃(120r/min)60h,,,;,,(10×100).:(1);(2);(3);(4);(5);(6);(7);(8)V.P;(9).2　2.1　　　1.1,,40h139　1　　　　　　,:　　　Fig.1　Changeofgrowthcycleoncompoundbacteria,43h,,,,.2.2　2.2.1　碳源含量随复合型微生物絮凝剂产生菌生长周期的变化　VIS-723G,:　ΔC=0.355;　R=1.000;　C=132.0A-6.343.2.2,0～56h,,,,2540.3mg/L242.6mg/L.,,3.2923mg/L.,,3.2923mg/L17.213mg/L,,,,,.2.2.2　氮源含量随复合型微生物絮凝剂产生菌生长周期的变化　,.,:ΔC=0.045;　　R=0.999;　　C=4.537A-0.494.3.3,.2,0～56h,,,42.22mg/L3.78mg/L,,,.,,、、,.,,,,,.Fig.2　ChangeofglucoseconcentrationingrowthcycleFig.3　Changeofnitrogensourceingrowthcycle2.3　pH,,,,.、,,,;,pH,、,,.pH4.4,,pH..2.4　5.5,24h,077.55%,,140　　()　　　45　.,60h97.35%.,.,,,.Fig.4　ChangeofpHingrowthcycleFig.5　Changeofflocculationactivityingrowthcycle2.5　,.6.6,3.02g/L,43%.5,,,,,.2.6　,3,,,7.,,92.4%.40h,,87h,,.Fig.6　ChangeofoutputingrowthcycleFig.7　Changeofcellflocculatedresultingrowthcycle2.7　、,1Brevibacteriummcbrellneri,;2Burkholderiaglumae,;3Cryptococcusalbidusvaraerius,.78.5%,79.3%,95.2%.38.Fig.8　Microscopemorphologiesofbacteria1(A),bacteria2(B)andbacteria3(C)31.1,141　1　　　　　　,:　　　,.、;、、H2S,V.P.3.Table1　PhysiologicalandbiochemicalcharactersofproductbacteriaReactionofphysiologyandbiochemistryPhenomena　　ExperimentonhydrolyzingasylumsItisunseentransparentcircleasiodineliquorwasdroppedaroundbacteriumgroup　　ExperimentonfluidizingglutinThespeedoffluidizingglutinbecamequickbypressureincreasedandfluidizedspeedofcompoundbacteriaismorequickthanhaploidone,it'soverin3d　　ExperimentonhydrolyzingfibrinNonin10d　　ExperimentofcheeseTransparentcirclewasappearedin12hcultureandmultiplebacteri-umismorequickthanhaploidone　　ExperimentofH2SNonofblacksedimentationwasappeared　　ExperimentofV.PExperimentmasculinepresented　　ExperimentofcontactenzymeItpresentedairbubbleafterHOwasaddedinto　　ExperimentofmilkwhichcurdlingtopeptoneFlocculatedbacteriumgrowinginmilkandnotcurdingtopeptone　　,,pH,pH=7;,,,;.,60h,97.35%,3.02g/L,43%.、,.[1]　ZHENGHuai-li,ZHANGHai-yan,QIANL.iResearchProcessofMicrobialFlocculants[J].ModernChemicalIndustry,2003,23(10):22-24.(,,　.[J].,2003,23(10):22-24.)[2]　.[M].:,1993.[3]　FujitaM,IkeM,HiraoT.Bioflocculation-productionfromLower-molecularFattyAcidsasaNovelStrategyforUtilizationofSludgeDigestionLiquor[J].WatSciTech,2001,44(10):231-236.[4]　MAFang,LIUJun-liang,LIShu-geng,eta.lDevelopmentofCompoundBioflocculant[J].ChinaWaterWasteWater,2003,19(4):1-4.(　,,,.[J].,2003,19(4):1-4.)[5]　CHENGShu-pei,CUIYi-bin,YANGLiu-yan.BreedingTechnologyonHighYieldFlocculantandStudyingProcess[J].AdvancesinEnvironmentalScience,1995,1(3):65-69.(,,.[J].,1995,1(3):65-69.)[6]　DONGDe-ming,KANGChun-li,