复合芽孢杆菌在海水养殖水体中脱氮的研究石玉新

JOURNALOFHEBEIUNIVERSITYOFTECHNOLOGY406Vol.40No.6201112December20111007-2373(2011)06-0034-06300130W01Y01W01Y0178%83%Y01W0182%70%W01Y01=1370%pH6.535200r/min80%X55ADigestinginorganicnitrogeninmariculturewaterwithcompositeBacillusSHIYu-xinQIShu-tingLÜYu-shanDUYun-fu(EngineeringResearchCenterofSeawaterUtilizationTechnologyofMinistryofEducation,HebeiUniversityofTechnology,Tianjin300130,China)AbstractTwokindsbacillusstrainsnamedW01andY01wereisolatedfromthemudofmarineaquaculturecompany.TheresultsshowthatammoniaremovalbyW01andY01strainswascarriedoutsimultaneouslywiththegrowth.Atsta-tionaryphase,ammonianitrogenremovalrateofW01andY01strainswere78%and83%.Y01wasunabletogrowinthemediumwherenitriteornitratenitrogenwastheonlynitrogensource,butnitriteandnitratenitrogenremovalratesofW01straincouldreach82%and70%.ThetwostrainswithdifferentdenitrificationcapacitieswerecombinedwhentheratioofW01Y01(containedincellnumberpermilliliterratio)was13,thecomplexstrainsontheinorganicnitrogenremovalwasupto70%.Withtheuseof9(34)orthogonalexperimentaltabletodeterminethebestconditionsforammoniaremovalbycomplexBacillus,speed200r/min,theammonianitrogenremovalofcomplexstrainswasupto80%undertheconditionsofpH6.5,temperature35.Keywordsammonia;inorganicnitrogen;denitrification;Bacillussp.;compositestrain[1][2-3][4](Paracoccusdenitrificans)(Alcaligenesfaecalis)(Pseudomonasputida)(Bacillussp.)(Arthrobactersp.)(Rhodococcus2010-11-15Z20094151968-DOI:10.14081/j.cnki.hgdxb.2011.06.010356sp.)[5-10],[11][12-14]pH11.11.23g10g1LpH7.0~7.210g3g3gMgSO40.1gK2HPO42g1LpH=7.85g5g5gK2HPO44gMnSO40.0154g1L3%NH42SO40.382g5gK2HPO40.2g1LpH=7.0~7.25g0.2gK2HPO40.1g1LpH=7.0~7.25g0.2gK2HPO40.1g1LpH=7.0~7.23%NH42SO40.367g0.2g1g0.1gK2HPO40.0175g1LpH=7.0~7.212125min1.3GB/T12763.4-2007GB/T12763.4-2007GB/T12763.4-2007OD7221.44g100mL120r/min3012h6025min101,102,103,104,1055104105100L303301.51.5.1100mL250mL30150r/min12h400~700nmOD36401.5.2100mL250mL30150r/min24h5%100mL250mL30150r/min3hOD6h=14/18+14()(1)mg/Lmg/Lmg/L1.5.3100mL250mL6h1.6108/mLW01Y0111122113315%30150r/min30h(1)1.7pH39341SPSS17.022.1W01Y0121Tab.1OrthogonalfactorleveltableApHB/C/rmin116.5157527.52515038.5352002W01Y01Tab.2W01andY01physiologicalandbiochemicalcharacteristicsofstrainsW01Y01++++-+++++++++376Y01W01Y01W01Y012.2W01Y012.2.1W01Y011600nmW01Y01600nmOD2.2.2W01Y01W01Y0122W01Y023h3~21h24hY01W012.2.3W01Y01W01Y01336~30h100mg/L36hW0178%Y0183%Y01W013W01Y01W0118h5.76mg/L24h48h0.87mg/LW01Y0148h22.14mg/LY01W01436hW0176.96%Y0176.14%W01Y012.2.4W01524h36h1W01Y01Fig.1ThemaximumabsorptionwavelengthofW01andY012W01Y01Fig.2ThegrowthcurveofW01andY01strainsinheterotrophicnitrificationliquidculturemedium3W01Y01Fig.3ThedegradationcurveofammoniaandtheaccumulationcurveofnitritenitrogenofW01andY01012243648120100806040200/h/(mgL1)W01W01Y01Y014005006007000.480.470.460.450.440.43/nmW01Y0109182736450.70.60.50.40.30.20.10/hW01Y014W01Y01Fig.4DegradationcurveofinorganicnitrogenofW01andY010122436481059075604530150/h/(mgL1)W01Y0138402.9mmol/LW0124h0.5mmol/L36h0.35mmol/LW0182%2.35mmol/LW0124h0.7mmol/L36h0.57mmol/LW0170%W0136h2.32.3.1W01Y01=11122113315%100mg/L40mg/L36h36h33Y0179.76%49.32%W0169.37%Y0167.19%Y01W01Y01=1378.21%Y0171.22%W01Y01W01Y01=13[15-17]336hTab.3Thecontentofthevariousnitrogenafter36hwhencomplexBacillusaccessedtheartificialwater/(mgL1)/(mgL1)/(mgL1)/(mgL1)/%/%W0131.639.474.3845.9369.3767.19Y0120.2438.1612.5570.9579.7649.321128.1825.4310.0163.6271.8254.561227.6215.378.3951.8372.3862.982126.1916.289.5252.9973.8162.151321.7912.436.7040.2978.2171.223125.2120.7410.4656.3174.7959.782.3.2436h40%RR4pHpH=6.535200r/min82.38%pH6.5~8.515~35pHpH5W01Fig.5W01strainofmediumnitritenitrogenandnitratenitrogencontentchangescurvewithtime06121824303642483.02.01.00/h/(mgL1)39631W01Y01W01Y012W01Y01W01Y01()=133934pH3pH=0.05[1]B115[J]20063369-11[2]XiaSLiJWangRNitrogenremovalperformanceandmicrobialcommunitystructuredynamicsresponsetocarbonnitrogenratioinacompactsuspendedcarrierbiofilmreactor[J]EcologicalEngineering2008323256-262[3]KimJKParkKJChoKSetalAerobicnitrificationdenitrificationbyheterotrophicBacillusstrains[J]BioresourceTechnology200596171897-1906[4][J]200727567-71[5][J]2009362255-260[6]Hung-SooJooMitsuyoHiraiMakotoShodaCharacteristicsofammoniumremovalbyheterotrophicnitrification-aerobicdenitrificationbyAlcaligenesfaecalisNo.4[J]TheSocietyforBiotechnology2005100209-212[7]CastignettiDHollocherTCHeterotrophicnitrificationamongdenitrifiers[J]ApplEnvironMicrobiol1984474620-623[8]DaumMZimmerWPapenHetalPhysiologicalandmolecularbiologicalcharacterizationofammoniaoxidationoftheheterotrophicnitrifierPseudomonasputida[J]CurrMicrobiol1998374281-288[9][J]200933395-399[10][J]20082731146-1150[11]TAYLORShaunaMHEYiliangZHAOBinHUANGJueHeterotrophicammoniumremovalcharacteristicsofanaerobicheterotrophicnitrifying-denitrifyingbacteriumProvidenciarettgeriYL[J]JournalofEnvironmentalSciences,20092171336-1341[12][J]200630988-91[13]De[J]20084315-19[14][J]200838626-30[15][J]2006374607-610[16]WXZ28/[J]200931717-24[17]JoongKyunKimKyoungJooParkKyoungSookChoetalAerobicNitrificationDe