复合藻菌净化系统处理麦秆造纸废液初步研究饶本强

DOI:10.3969/j.issn.1003-0972.2009.04.031-饶本强1,2＊,张列宇2,陈　坤1(1.,464000;2.,430072)　:在室内模拟条件下,采用正交设计法建立了一种由蛋白核小球藻(Chlorellapyrenoidosa)、鱼腥藻7120(Anabaenasp.PCC7120)、硝化细菌(Nitratebacteria)和荚膜红假单胞菌(Rhodopseudomonascapsulata)组成的复合藻-菌净化系统去除造纸废液中有机质和氨态氮的最优化模型,确定了藻类与细菌的最优化数量配比关系为蛋白核小球藻∶鱼腥藻7120∶硝化细菌∶荚膜红假单胞菌=1∶2.17∶2.83∶5.09.比较了复合藻-菌净化系统最优化模型与单藻、单菌去除造纸废液中有机质和氨态氮的代谢速率的差异.结果显示,复合藻-菌系统代谢有机质、氨态氮的速率远高于单藻、单菌,其去除率分别为91.3%、79.2%;39.9%、22.7%;58.6%、68.9%.研究了光照强度和温度等生态因子对复合藻-菌净化系统最优化模型代谢有机质和氨态氮的影响.结果表明,在光照与黑暗条件下,复合藻-菌系统代谢有机质和氨态氮的速率存在明显差异,黑暗有利于有机质代谢而光照利于氨态氮的去除.藻-菌系统对有机质和氨态氮的代谢能力与系统的环境温度相关.:复合藻-菌净化系统;麦秆造纸废液;藻-菌数量配比;有机质代谢;氨态氮去除:S932.9　　:A　　:1003-0972(2009)04-0593-05PrimaryStudiesonPurificationEffectsofTreatmentModelofAlga-bacteriaSystemonWheatStalkPaper-makingWasteWaterRAOBen-qiang1,2＊,ZHANGLie-yu2,CHENKun1(1.CollegeofLifeSciences,XinyangNormalUniversity,Xinyang464000,China;2.StateKeyLaboratoryofFreshwaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China)Abstract:Laboratoryexperimentswereconductedtoinvestigateamostoptimizedpurificationmodelofalgae-bac-teriasystemwhichconsistedofChlorellapyrenoidosa,Anabaenasp.PCC7120,NitratebacteriaandRhodopseudomonascapsulateappliedtoremoveorganicmatter(CODcr)andNH+4-Ninwheatstalkpaper-makingwastewaterbymeansoftheorthogonaltestdesign,andmostoptimizedratioofalgaeandbacteria(i.e.,Chlorellapyrenoidosa∶Anabaenasp.PCC7120∶Nitratebacteria∶Rhodopseudomonascapsulate)was1∶2.17∶2.83∶5.09.ThedifferencesofmetabolicratesforremovalofCODcrandNH+4-Nbetweenthemostoptimizedpurificationmodelofalgae-bacteriasystem,thesingleal-gaeandthesinglebacteriawerecompared.Theresultsindicatedthattherewerehighermetabolicratesforalgae-bacte-riasystemtoremoveCODcrandNH+4-Nthanthoseofthesinglealgaeandthesinglebacteria,andtheirremovalrateswere91.3%,79.2%,39.9%,22.7%,58.6%and68.9%,respectively.Theeffectsofsomeecologicalfactorsuchasilluminationintensityandtemperatureonthealgae-bacteriasystemappliedtoremoveCODcrandNH+4-Nwereinvestigated.ItshowedthattherewasobviousdifferenceinthemetabolicratesofCODcrandNH+4-Nbetweendarkcon-ditionandlightconditionwhenthealgae-bacteriasystemwasusedtodealwithwheatstalkpaper-makingwastewater,andtheconditionoflightwasprovedtoavailtoremoveNH+4-Nforthealgae-bacteriasystembutthedarkconditionwasmorebeneficialforthesysteminremovingCODcr.Also,itwasfoundinthestudythatthemetabolicrateofthealgae-bacteriasystemforremovalofCODcrandNH+4-Nwascorrelativewithtemperature.Keywords:algae-bacteriapurificationsystem;wheatstalkpaper-makingwastewater;algaeandbacteriaamountratios;metabolismoforganicmatter;removalofNH+4-N　:2009-04-07;:2009-07-31;＊.:E-mail:[e-mail]　:(20060206);　:(1974-),,,,,.·593·:JournalofXinyangNormalUniversity22　4　200910NaturalScienceEditionVol.22No.4Oct.2009　　,.,、,,、、COD[1]..,.,、[2].、、,,[3].,-.,、、,-.、,,-,-、,-.1　1.1　1.1.1　藻、菌来源及其增殖培养(Chlorellapyrenoidosa)7120(Anabaenasp.PCC7120)(FACHB-Collection),2、.(Rp.capsulata)(Nitratebacteria).RCVBN[4].、.7120BG-11[5]、25±1℃、40μE·m-2·s-1、.HB-4[6],25±1℃、60μE·m-2·s-1、.1.1.2　实验用水制备,.CODcr125.96mg·L-1,NH4+-N24.38mg·L-1[7].1520min4℃.1.21.2.1　复合藻-菌净化系统最优化模型的建立434、-,-(1).1mL、7120、、,1.12×106cfu·mL-1、1.03×106cfu·mL-1、1.46×106cfu·mL-1、1.98×106cfu·mL-1;,2mL/,3mL/.500mL/2000mL,(PGX,),28℃、30μE·m-2·s-1.9-,10h,/CODcrNH4+-N.1.2.2　藻、菌代谢实验-()、(+7120)、(+).200mL1000mL,28℃、40μE·m-2·s-148h,/CODcrNH4+-N,CODcrNH4+-N./.3.1.2.3　光照强度对复合藻-菌净化系统最优化模型的作用()、40μE·m-2·s-1、100μE·m-2·s-13,-,72h.CODcrNH4+-N,.(Li-185B、Li-COR,·594·22　4:　)./.1.2.4　温度对复合藻-菌净化系统最优化模型的影响10、20、30℃3,-48h.12h,CODcrNH4+-N(mg·L-1·h-1)./.1　-(×106cfu·mL-1)Tab.1Amountratiosofwaterpurificationmodelofalgae-bacteriasystembasedonorthogonaltestdesign712011(1.03)1(1.12)1(1.46)1(1.98)1∶1.09∶1.42∶1.7022(2.06)1(1.12)2(2.92)2(3.96)1∶0.54∶1.42∶1.7033(3.09)1(1.12)3(3.38)3(5.94)1∶0.36∶1.42∶1.7041(1.03)2(2.24)2(2.92)3(5.94)1∶2.17∶2.83∶5.0952(2.06)2(2.24)3(3.38)1(1.98)1∶1.08∶2.12∶0.8563(3.09)2(2.24)1(1.46)2(3.96)1∶0.72∶0.47∶1.1371(1.03)3(3.36)3(3.38)2(3.96)1∶3.26∶4.25∶3.4082(2.06)3(3.36)1(1.46)3(5.94)1∶1.63∶0.71∶2.5593(3.09)3(3.36)2(2.92)1(1.98)1∶1.09∶0.94∶0.572　2.1　-4、-,-(2).,7120、、、,:7120∶∶=1∶2.17∶2.83∶5.09-,.、,,,.-,,,.,,[8-9].,-、,.,-,、,,-[2,10].2　-(mg/L)Tab.2Effectsofwaterpurificationmodelsofalgae-bacteriasystemindifferentamountratiosonremovingCODcrandNH4+-N(mg/L)/hC1C2C3CODcrNH+4-NCODcrNH+4-NCODcrNH+4-N0126.0124.40125.9324.36125.9924.381292.7820.3892.4420.4598.0521.202461.2517.1357.8116.4169.1718.253647.1212.8443.2611.9855.3613.694839.865.2635.097.6347.598.616018.432.1919.022.7718.963.05/hC4C5C6CODcrNH+4-NCODcrNH+4-NCODcrNH+4-N0125.9524.39126.0324.41125.9424.371290.7320.1295.7820.3899.8621.252453.9616.3764.2517.2373.5818.043638.2412.5950.1113.8461.2614.994831.153.3039.8610.2647.2211.306013.820.9522.435.1225.016.02/hC7C8C9CODcrNH+4-NCODcrNH+4-NCODcrNH+4-N0125.9624.35125.9124.33125.9524.391293.8220.4693.0620.5298.1221.302454.9816.3961.5316.4177.0118.123647.1512.0849.2812.1465.9414.724838.033.4241.067.5556.3811.056016.751.2919.333.2323.495.562.2　-、1,-、,-、.91.3%、79.2%;39.9%、22.7