高浓度基质冲击后厌氧氨氧化菌活性恢复研究杨瑞丽

38720187ActaScientiaeCircumstantiaeVol．38No．7Jul．2018No．2015J05115No．IUEMS201404SupportedbytheNaturalScienceFoundationofFujianProvinceNo．2015J05115andtheFrontierＲesearchProjectofIUE－CASNo．IUEMS2014041989—[e-mail]*[e-mail]1989—[e-mail]*[e-mail]10．13671/j．hjkxxb．2017．0518．2018．J．3872615-2621YangＲLWangXJGuoYetal．2018．StudyontheactivityrecoveryofanammoxbacteriainfluencedbythehighconcentrationmatrixJ．ActaScientiaeCircumstantiae3872615-2621121121*1．3610212．1000492017-11-202017-12-262017-12-26Anammox、EPSAnammox．Anammox57d2．21kg·m－3·d－1AnammoxEPS、EPSEPSTN500mg·L－1EPSAnammoxTN700mg·L－12．4×1010copies·g－1VSS．EPS0253-2468201807-2615-07X172AStudyontheactivityrecoveryofanammoxbacteriainfluencedbythehighconcentrationmatrixYANGＲuili12WANGXiaojun1GUOYan12CHENShaohua1*1．CASKeyLaboratoryofUrbanPollutantConversionInstituteofUrbanEnvironmentChineseAcademyofSciencesXiamen3610212．UniversityofChineseAcademyofSciencesBeijing100049Ｒeceived20November2017receivedinrevisedform26December2017accepted26December2017AbstractTheactivityrecoveryperformanceoftheanaerobicammoniumoxidationAnammoxbioreactorwhichhadsufferedfrominhibitionofhighsubstratewasinvestigatedlongtermdynamicchangeextracellularpolymersEPScontentsandabundanceofanammoxbacteriainanammoxreactionwereevaluatedinthisstudywithstagewiseimprovingnitrogenloadingrate．Ｒesultsshowedthattherecoveryexperimentofanammoxreactorwassucceedandfinalnitrogenremovalratewasapproximately2．21kg·m－3·d－1within57daysitisbetterthanthenitrogenremovalperformanceofthereactorbeforedamagedWheninfluentconcentrationoftotalnitrogenwaslessthan500mg·L－1thecontentofEPSratioofproteinandpolysaccharidePN/PSofbothtightlyboundTBEPSandlooselyboundLBEPSweredecreasedgraduallywhiletheconcentrationwas500～1000mg·L－1thecontentofEPSTB-PN/PSandLB-PN/PSwereincreasedslightlyAbundanceofanammoxbacteriawaschangedinvolatilitywiththepeakof2．4×1010copies·g－1VSSat700mg·L－1asforthehighsensitivitytonitrogenofwastewaterthehighest．KeywordsanammoxactivityrecoveryabundanceofanammoxbacteriaEPS1IntroductionAnaerobicammoniumoxidizingAnammoxNO－2NH+4N2Wretal．2007．Anammox、、Jinetal．2012a2012bZhangetal．2013．Anammox、、Jinetal．2012bWretal．2007Anammox．38Heetal．2016Lietal．2017bZhangetal．2013NO－2-NNH+4-NAnammoxAnammoxAnammoxLietal．2017a2012b．NO－2-NAnammoxNH+4-NPuyoletal．2014Carvajalarroyoetal．2015NH+4-N1000mg·L－1AnammoxNO－2-N280mg·L－1NO－2-NAnammoxDapena-Moraetal．2007Isakaetal．2007Wangetal．2016Strous1999．AnammoxAnammox．ExtracellularPolymericSubstancesEPSEPSTB-EPSLB-EPSBadireddyetal．2010Shengetal．2010Zhuetal．2009．EPSProteinPNPolysaccharidePSPSEPSPN、EPSPSEPSFlemmingetal．2010．PNPS、Houetal．2015Yinetal．2015Zhuetal．2009Anammox．Anammox、EPSAnammoxAnammoxAnammox．2Materialsandmethods2．11．1．5L4．35±2℃pH0．1mol·L－17．5Anammox．1Fig．1Schematicdiagramoftheexperimentaldevice2．21．g·L－1EDTA20、ZnSO4·7H2O0．43、CoCl2·6H2O0．24、MnCl2·4H2O0．99、CuSO4·5H2O0．25、NaMoO4·2H2O0．043、NiCl2·6H2O0．20、KH2PO420、H3BO30．014．0．45μm、N-1-NH+4-N、NO－2-NNO－3-NHendrickxetal．2014．pHpHFG2-FKMETTLEＲTOLEDOUSAMLSS、MLVSS《》2002．616271Table1ThecompositionofsyntheticwastewaterNH4Cl2NaNO22KHCO31．25g·L－1MgSO4·7H2O0．2g·L－1FeSO4·7H2O0．023g·L－11．25mL·L－11．TNNH+4-N350mg·L－1NO－2-N350mg·L－1700mg·L－1HＲT9hNH+4-NNO－2-N94%83%．TN700mg·L－1NO－2-N20．92mg·L－1320．24mg·L－11NH+4-NNO－2-N9．67%8．75%MLSS7．46g·L－1MLVSS3．96g·L－1．Anammox5TN160、320、500、7001000mg·L－12．2Table2Ｒeactoroperationparametersofeachphase/dHＲT/hNH+4/mg·L－1NO－2/mg·L－1Ⅰ1～12128080Ⅱ13～171216016018～259Ⅲ26～369207273Ⅳ37～479302398Ⅴ48～5794315692．3EPSEPSYuetal．20094℃1．5hNa3PO4∶NaH2PO4∶NaCl∶KCl=2∶4∶9∶1pH=7．04℃2000g15min2000g15minLB-EPS20kHz、480WScienta-IIDChina10min1s-2s4℃2000g20minTB-EPS．LB-EPSTB-EPS0．45μm．EPSBCAP0010S2015-2016．2．4DNAPCＲ500mgFastDNATMSPINKitforSoilLLCMPBiomedicalsUSADNA．PCＲＲocheLightCycler480ⅡＲocheDiagnosticsLtdＲotkreuzSwltzerland20μLSYBＲGreenⅠMasterLightCycler480mannheimGermany10μL0．8μLDNA1μL7．4μL．341F534ＲHouetal．2014AnammoxAmx808F5'-AＲCYGTAAACGATGGGCACTAA-3'Amx1040Ｒ5'-CAGCCATGCAACACCTGTＲATA-3'Houetal．2014Mengetal．2010．PCＲ95℃5min95℃30s45℃30s72℃30s3572℃10min．3Ｒesultsanddiscussion3．123aNO－3-NNO－2-NNH+4-N5mg·L－148hNH+4-N．TNⅠ～Ⅱ91．74%Ⅲ～Ⅴ78．8%．NO－2-NⅠ～Ⅱ98%Ⅲ～ⅤⅤ83．8%．NH+4-N98%．3bⅠ～ⅤNitrogenＲemovalＲateNＲＲNitrogenLoadingＲateNLＲ716238NＲＲ0．21、0．64、1．05、1．552．21kg·m－3·d－1Zhang2015Anammox．NLＲShengetal．2010Anammox．23Ⅰ～ⅣNLＲ24hⅣ．Ⅱ18dΔNO－3-N/ΔNH+4-NⅢHＲT12h9h．ⅤNLＲ72hNH+4-NNO－2-NⅣ2．44%10．23%NO－2-NAnammoxCODTianetal．2013．NO－2-N/NH+4-N1．32Ⅰ～ⅤNH+4-N96%NO－2-NΔNO－3-N/ΔNH+4-NNO－2-N0．79mg·L－191．00mg·L－1NO－2-NAnammoxNO－2-NAnammoxFernándezetal．2012Kimuraetal．2010Tangetal．2010．NO－2-N750mgL－190%AnammoxKimuraetal．2010．1000mg·L－1TNNH+4-N+NO－2-N50%AnammoxLottietal．2012．2Fig．2Nitrogenvariationofinfluentandeffluentduringrecoverystageofanammoxreactor3a、bFig．3NitrogenremovalperformanceoftheanammoxreactorincludingnitrogenremovalefficiencyanitrogenloadingrateΔNO－2-N/ΔNH+4-NandΔNO－3-N/ΔNH+4-Nb3．2EPS4EPSNLＲⅠ～ⅤEPS150．56、33．51、8．42、10．0510．21mg·g－1VSSTB-EPSLB-EPSTB-EPSLB-EPSPNPSJia2017Pellice