高效原油降解菌的筛选及其在生物活性炭中的应用冯晋阳

:,,1973,,。＊(No.07JK290)。高效原油降解菌的筛选及其在生物活性炭中的应用＊冯晋阳　吴小宁(,　710032)　　　,、6SY1～SY6,55%。,SY1(Microbacteriumsp.),SY2(Nocardiasp.),SY3SY5(Pseudomonassp.),SY4SY6(Bacillussp.)。SY2、SY4、SY5SY6SY,SY(BAC),BAC,COD75%,80%,。　　　　　　Screeningofhighefficientoil-degradingstrainsandtheirapplicationinbiologicalactivatedcarbon　FENGJinyang,WUXiaoning.(SchoolofMaterialandChemicalEngineering,Xi'anTechnologicalUniversity,Xi'anShaanxi710032)Abstract:　Sixoildegradingstrains(namedSY1toSY6)wereisolatedandscreenedfromoil-pollutedsoilwithcrudeoilassinglecarbonsource.Theoildegradingrateofthesixstrainswereallhigherthan55%.Sixstrainswereidentifiedbaseontheirphysiologicalcharacteristicsandmorphologyobservation,resultsshowedthatSY1belongedtoMicrobacteriumsp.,SY2belongedtoNocardiasp.,SY3andSY5belongedtoPseudomonassp.,SY4andSY6be-longedtoBacillussp..SY2,SY4,SY5andSY6wereselectedfortheconstructionofoildegradationbacterialcom-munitySY.ThebacterialcommunitySYwasinoculatedintothebiologicalactivatedcarbon(BAC)reactorfortreat-mentofoilcontainingwastewater,BACreactorpresentedperfecttreatmentperformanceafterthestableoperation,theremovalrateofoilandCODwereupgo80%and75%,respectively.Keywords:　oil-pollutedsoil;oildegradationbacterium;screening;biologicalcarbon　　,,,[1]。,、、、[2-4]。,,。,,[5,6]。,10mg/L。,。　　,,[7]。,,。,,。,,。(BAC),,,。[8][9]BAC,。　　、,BAC,,,。1　1.1　原油降解菌的筛选1.1.1　2.5kg,24h,2h·39·　DOI:10.15985/j.cnki.1001-3865.2011.06.011,,。　　、,。(60～90℃),,100mg/mL。1.1.2　:NaCl10g,NH4Cl0.50g,KH2PO40.50g,K2HPO41.0g,MgSO40.50g,CaCl20.02g,KCl0.10g,FeCl2·4H2O0.02g,1000mL,1mL,pH=7.2。　　:+。　　:1000mL,3g,18g。,。　　:1g,10g,NaCl5g,3g,15g,1000mL,pH7.2～7.4。,。　　100mL250mL,,10mL,200r/min、30℃,。3d。,30℃5d,5。。,,4℃。1.1.3　。　　500mg/L,50mL200mL,30℃、200r/min,((OD))(),、,。1.1.4　,,[10,11],,。1.2　BAC对含油废水的处理1.2.1　,,SY。SYBAC。1.2.2　,1。1　Table1　CharacteristicsofrawwaterCOD/(mg·L-1)/(mg·L-1)pH100～12010～157～91.2.3　BAC,,2。2　Table2　ParallelcontrolgroupSYBACSY　　,75mL,100mg/L,SY(5mL)(20mL),30℃,pH7～8。5d,,。3,。1.2.4　1。BAC15cm,57cm。BAC,。0.9～1.1mm。30℃,pH7～8。1　Fig.1　Schemeticdiagramofexperimentapparatus1.3　分析方法751G227nm(A227),·40·　　33　6　20116(c,mg/L),(η,%):η=(c0-c)/c0×100%(1):c0,mg/L。　　722460nm,,OD[12]。　　COD。2　2.1　高效原油降解菌筛选结果2.1.1　13(1#～13#),。13。(30℃)5d,OD,3。3　Table3　Thegrowthofstrainsandtheiroildegradationcapacity(OD)/%(OD)/%1#0.05922.38#0.09559.92#0.07233.19#0.09967.63#0.07030.910#0.07938.34#0.12066.111#0.05112.55#0.08355.612#0.12876.26#0.09157.113#0.05619.77#0.06516.70.0052.8　　3,930%,9#12#(OD),,67%。,,55%6(4#、5#、6#、8#、9#、12#,SY1～SY6),6。2.1.2　,,6,4。2.2　高效原油降解菌对含油废水的处理结果SY2、SY4、SY5SY6SY[13]。2.2.1　BAC,2。　　2,,SY,,。,,BAC,BAC。,SY,,SY2d45.0mg/L,62.0mg/L,BACSY4　6Table4　TheidentifyingcharacteristicsofthesixoildegradationstrainsSY1SY2SY3SY4SY5SY6,,,,,,,,,,,,,,,,,,/μm(0.1～0.2)×(0.4～0.6)(0.5～0.6)×(2.0～2.5)(0.2～0.3)×(1.0～1.5)(0.5～0.6)×(1.0～1.2)(0.3～0.5)×(1.2～1.5)(0.5～0.6)×(0.9～1.3)Microbacteriumsp.Nocardiasp.Pseudomonassp.Bacillussp.Pseudomonassp.Bacillussp.·41·　2　Fig.2　Theresultsofparallelcontrolgroupexperimental,36.0mg/L,。3d,,;SYBAC,SY,。,BACSY,BAC,SY,,。　　5d,:25.0%,SY83.1%,65.0%,BAC91.5%。BAC。2.2.2　BAC3。3,BAC3。,,26.5mg/L。,BAC,,BAC。,,,。26,3　Fig.3　Thevarietyofoilcontentandoilremovalrate,,,,。(640),2.5mg/L,80%。2.2.3　CODCOD4。4,COD。BAC,COD230mg/L,。COD,6,25mg/L。,,COD。,COD,75%。4　CODFig.4　ThevarietyofCODandCODremovalrate2.2.4　5。5,,,1d,,5,,,5　Fig.5　Thevarietyofbiomass·42·　　33　6　20116。,,。　　,。,,,,,。,,,,,BAC。3　　(1)6(SY1～SY6),55%。,SY1、SY2、SY3SY5、SY4SY6。(2)BAC,SY。,,,。BAC,80%,COD75%,。:[1]　,,,.[J].,2008,34(10):26-29.[2]　ALFUTAISIA,JAMRAHA,YAGHIB,etal.AssessmentofalternativemanagementtechniquesoftankbottompetroleumsludgeinOman[J].JournalofHazardousMaterials,2007,141(3):557-564.[3]　MACHNRAMREZC,OKOHAI,MORALESD,etal.Slur-ry-phasebiodegradationofweatheredoilysludgewaste[J].Chemosphere,2008,70(4):737-744.[4]　,,,.[J].,2008,27(8):1155-1161.[5]　.[J].,2006,14(3):34-38.[6]　POULOPOULOSSG,VOUTSASEC,GRIGOROPOULOUHP,etal.Strippingasapretreatmentprocessofindustrialoilywastewater[J].JournalofHazardousMaterials,2005,117(2/3):135-139.[7]　,.[J].,2008,27(1):230-233.[8]　,,,.[J].,2004,20(2):187-190.[9]　,,,.[J].,2006,27(5):918-923.[10]　,.[J].,2008,34(3):9-12.[11]　.[M].:,1987.[12]　,,.[J].,2000,19(7):325-329.[13]　,.[J].,2009,32(3):57-61.编辑:陈泽军　(:2010-12-23)(上接第38页),Al3+0.8g/kg,、,,;Al3+,,,Al3+。(2)pHAl3+。(3)pHAl3+,pH,Al3+。。:[1]　.[J].,1998,17(4):178-180.[2]　,,,.[J].,2009,20(7):1698-1704.[3]　,.[J].,2005,31(2):111-118.[4]　,.[J].,2009,25(13):94-98.[5]　HAMELRD,APPANNAVD.ModulationofTCAcycleen-zymesandaluminumstressinPseudomonasfluorescens[J].J.Lnorg.Biochem.,2001,87(1/2):1-8.[6]　CHENIERD,BERIAULTR,MAILLOUXR,etal.Involve-mentoffumaraseCandNADHoxidaseinmetabolicadaptationofPseudomonasfluorescenscellsevokedbyaluminumandgal-liumtoxicity[J].Appl.Environ.Microbiol.,2008,74(13):3977-3984.[7]　PINARG,CERVANTESC.Microbialinteractionswithalu-minium[J].BioMetals,1996,9(3).[8]　.[M].3.:,2005:30-86,106-163.[9]　,.[M].:,1986:102-125.[10]　,,.[J].,2005,36(5):735-738.[11]　,,,.[J].,2003,23():16-20.[12]　,,.[J].,2009,25(5):42-45.编辑:卜岩枫　(:2011-03-23)·43·