高盐废水处理的耐盐菌株及其高效除磷特性研究邓若男

880(51208007):20120921;:20121106;:20130528:(),49,5,20139ActaScientiarumNaturaliumUniversitatisPekinensis,Vol.49,No.5(Sept.2013)邓若男陈倩倪晋仁†,,100871;†,E-mail:[e-mail]-116SrRNA,(Salinivibriosp.)HG-1,1%~13%,3%,4HG-1:pH,pH6.5~7.0,30,10%,9,24100%,,,;;X172EfficientPhosphorusRemovalfromWastewaterbyaNewlyIsolatedBacteriumunderHighSalinityConditionDENGRuonan,CHENQian,NIJinren†KeyLaboratoryofWaterandSedimentSciences(MOE),DepartmentofEnvironmentalEngineering,PekingUniversity,Beijing100871;†Correspondingauthor,E-mail:[e-mail]tionand16SrRNAgenesequenceanalysis,theisolatewasidentifiedasSalinivibriosp.(namedHG-1).Salinitytoleranceandphosphorusremovalefficiencyunderdifferentsalinityconditionsofthestrainwerefurtherinvestigated.TheresultsshowedthatHG-1grewwellwiththesaltcontentvaryingfrom1%to13%andachievedthehighestphosphorusremovalefficiencyundersaltcontentof3%.Furthermore,thesingle-factorandorthogonalexperimentresultsindicatedthattheoptimalphosphorusremovalperformancewasobtainedundertheconditionswithaninitialpHof6.5–7.0,C/Nratioof9,temperatureof30°Candinoculationratioof10%.Undersuchacondition,thephosphorusremovalefficiencycouldreach100%in24hours.ThestrainHG-1canindependentlycompletephosphorusremovalprocess,andthuscouldprovideanovelandpromisingalternativeforbiologicalphosphorusremovalunderhighsalinityconditions.Keywordsphosphorusremoval;highsalinitywastewater;salttolerantstrain(NaCl)1%,,,Cl−,Na+,Ca2+SO42−,,[1–5],,,,Woolard[6]DOI10.13209/j.0479-8023.2013.117881SBR,,HG-1,16SrRNA,,,11.1A:0.94g/L,0.153g/LNH4Cl,0.035g/LKH2PO4,0.1g/LMgSO4·7H2O,0.006g/LFeSO4·7H2O,30~150g/LNaCl,pH7.0~7.5B:5g/L,10g/L,30~150g/LNaCl,pH7.0~7.52%A,,B,,1.21.2.1:1mL100mLB250mL,30150r/min(DDHZ-300,)5,10−1,10−2,…,10−6,10−4,10−510−60.1mL,(NaCl)3%,5%,8%,10%15%B(SPX,)30,,,B,,,HG-1HG-11LB,8,,30150rpm,,3,(OD600)1~21.2.21mL,0.2mLB,,3,30,HG-1,(Nikon,)5mL,DNADNAPCRPCR(50μL):1μLDNA,1μL27F(5’-AGAGTTTGATCCTGGCTCAG-3’),1μL1492R(5’-GTATTACCGCGGCTGCTGG-3’),0.25μLDNA(TaKaRaPremixTaqTMHotStartVersion),5μLdNTP(10mmol/L),5μL10×PCR,50μLPCR:95C5,94C155C3072C30,30,72C8PCR1%(Biorad,),−20C1.2.3104B,303,100,+++;100,50,++;50,+;,−1.2.410mL(NaCl)1%,3%,5%,8%,10%13%100mLA,30,150rpm,C/N(/)9,pH6.5~7.0,(KH2PO4-P)10mg/L3,62mL,8000rpm10,8821.2.5:pH(6.5~7.0,7.0~7.5,7.5~8.0);(20,30,40);C/N(3.7,7.5,9)(5%,10%,15%),10mL(NaCl)3%100mLA,30,150rpm,10mg/L1.2.41.2.6(GB1189389)(OD600)(SHIMADZU,)600nmpHpH201pH(HANNA,),pH22.1HG-1,,,(0.6~1.2)μm×(1.5~6.0)μm,(1)BLAST()HG-116SrRNA(1409bp)GenBank16SrRNA,HG-1Salinivibriosp.,99%,(Salinivibriosp.)2.2HG-11,1%~10%NaCl1HG-1Fig.1OpticalmicrographofstrainHG-11Table1CapabilityofNaCltolerancebystrainHG-11%NaCl3%NaCl5%NaCl8%NaCl10%NaCl13%NaCl15%NaClHG-1+++++++++++++++++−:+++,++,+,−,13%NaCl,15%NaCl,HG-12.3HG-12,HG-110mg/L,1%,3%,5%,8%,10%13%,247.27,0.00,1.35,2.47,2.345.06mg/L;,HG-1,48HG-126.08%,77.00%,54.74%,57.20%,67.70%31.51%1%~13%,HG-1281.70%;3%,HG-1,24100%24,HG-1,,:,,;2HG-1Fig.2EffectsofchlorideconcentrationonphosphorusremovalbyHG-1883;,Uygur[7]SBR,,06%,84%22%[8],HG-11%~13%,,2.4HG-1,pHC/N2.4.1pHpH3,pH6.5~7.0,7.0~7.57.5~8.0,482.30,2.538.10mg/LpH6.5~7.0,HG-124100%;pH7.5~8.0,HG-14818.99%,HG-1pH,pH7.5~8.0,pH6.5~7.02.4.24,HG-1,20,3040,482.27,2.302.83mg/L30,HG-1,24100%2.4.3C/N5,C/N3.7,7.59,483HG-1pHFig.3PhosphorusremovalbystrainHG-1atdifferentpHvalues4HG-1Fig.4PhosphorusremovalbystrainHG-1atdifferenttemperatures4.04,3.162.30mg/L;C/N9,HG-1C/N3.77.5,C/N,HG-1,2.4.465%,10%15%,481.71,2.302.80mg/L;10%,HG-15HG-1C/NFig.5PhosphasremovalbystrainHG-1atdifferentC/Nvalues6HG-1Fig.6PhosphorusremovalbystrainsHG-1underdifferentinoculations8845%15%,2.4.5pHC/NHG-1,,,,,2,pHC/N4HG-1:pHHG-1A1B2C3D2,pH6.5~7.0,30,9,10%pH6.5~8.0,pH,,C/N,,3.77.5,,C/N7.59,20~40,5%15%,,,HG-1,,HG-13HG-1,(Salinivibriosp.)HG-11%~13%NaCl;,81.70%3%,24100%4HG-1:pH,pH6.5~7.0,30,10%,9,,[1],..,1999,19(5):17–18[2],,,.NaCl.,2004,22(1):19–21[3],,,..,1999,20(3):104–106[4],,..,2008,27(3):89–92[5],,,..,2002,24(5):293–294[6]WoolardCR,IrvineRL.Treatmentofhypersalinewastewaterinthesequencingbatchreactor.WaterResearch,1995,29(4):1159–1168[7]UygurA,KargiF.Saltinhibitiononbiologicalnutrientremovalfromsalinewastewaterinasequencingbatchreactor.EnzymeandMicrobialTechnology,2004,34:313–318[8]YoshieS,MakinoH,HirosawaH,etal.Molecularanalysisofhalophilicbacterialcommunityforhigh-ratedenitrificationofsalineindustrialwastewater.AppliedMicrobiologyandBiotechnology,2006,72(1):182–1892HG-1()Table2ResultsoforthogonalexperimentofHG-1(phosphasremovalefficiency)HG-1ABCD24pHT/C/N/%TP/%11(6.5~7.0)1(20)1(3.7)1(5)53.0921(6.5~7.0)2(30)2(7.5)2(10)93.8431(6.5~7.0)3(40)3(9)3(15)92.4942(7.0~7.5)1(20)2(7.5)3(15)58.8352(7.0~7.5)2(30)3(9)1(5)80.9462(7.0~7.5)3(40)1(3.7)2(10)53.6573(7.5~8.0)1(20)3(9)2(10)29.3783(7.5~8.0)2(30)1(3.7)3(15)25.0893(7.5~8.0)3(40)2(7.5)1(5)31.1710.7980.4710.4390.55120.6450.6660.6130.59030.2850.5910.6760.5880.5130.1950.2370.039