铬离子对序批式好氧生物反应器出水中溶解性微生物产物的影响

26120071ENVIRONMENTALCHEMISTRYVo.l26,No.1January2007(2006810).*(50478068).**:gthuang@ecusteducn*黄光团**金刚吴景辉刘勇弟(,,200237)(SBR),(SMP)DNA.2mgl-1,SMP:SMP=2494e0593C.SMP,1kDa50%.SMPDNA,=005,DNA.,,,,,DNA.,(SMP)[15].SMP,,[6].SMP,CODBOD,;.,SMP[7],.(SBR),SMPDNA,SMP,.111,,,.1000mgl-1(COD),(SRT)7d,35,pH68(DO)47mgl-1.,(1L),,,,.SMP,,34,SMP.937mgl-1,COD1000mgl-1,NH4Cl200mgl-1,KH2PO4400mgl-1,Na2HPO4,630mgl-1,CaCl220mgl-1,MgSO47H2O20mgl-1,FeCl36H2O05mgl-1.pH,.24h,.1000,(68h),;,2h15min,830,1000.,1:67,24h.12045m,.:045m,1kDa10kDa,(TOC1)(TOC2,TOC3).:MW1kDa(TOC2TOCR),1kDaMW10kDa(TOC3TOC2),MW10kDa(TOC1TOC3),SMP(TOC1TOCR).TOCR,MW1kDa.DNA,045m.:[8].(Bradford)[9].DNA[10].[11].2210520mgl-1,30d,.:30mgl-115d,50mgl-112d,70mgl-110d,110mgl-18d,150mgl-14d.,,2mgl-1,,2mgl-1.1,(30d).1,,.,,,,.,,7d,,,,,,,,,.1Fig1RelationshipofconcentrationsofCr6+andremovalefficiencyofsubstrate22SMP1,SMPSMP.68261SMPTable1AmountandmolecularweightdistributionofSMPundervariousconcentrationsofCr6+Cr6+(mgl-1)SMP(mgl-1)1kDa1kDa10kDa10kDaTOC/mgl-1/%TOC/mgl-1/%TOC/mgl-1/%0253713275234671847432930533111734523641194936283104551217847910082211365300155795339358510541821348233208445595770610761271412167SMP,1,SMP.SMP,,SMP:SMP=2494e0593C,R=09978.SMP,1,SMP,10kDa1kDa,1kDa50%.05mgl-1,,,,SMP.20mgl-1,,,,,,,.23SMPDNA2,SMPDNA.2,=005,n=5,R005,3=0878.2DNA,3.2SMPDNATable2Contentsofpolysaccharide,proteinandDNAundervariousconcentrationsofCr6+Cr6+/mgl-1005101520/mgl-11816181916/mgl-12725303030DNA/mgl-1144140126109993SMPDNATable3CorrelativeofconcentrationsofCr6+withcontentsofpolysaccharide,proteinandDNAinSMPRR005,3RR005,3Y=-002C+176011790878Y=022C+262075550878DNAY=-242C+14780984908782,,DNA.3,,=005,n=5,SMP,DNA,,DNA,,,,,,1:69DNA,DNA.,DNA,,DNA.32mgl-1.,SMP:SMP=2494e0593C.SMP,10kDa1kDa,1kDa50%.=005,n=5,SMP,DNA,.[1]NogueraDR,ArakiN,RittmannBE,SolubleMicrobialProducts(SMP)inAnaerobicChemostats.Biotech.Bioeng.,1994,44(9)10401047[2]GaffneyPE,HeukelekianH,BiochemicalOxidationoftheLowerFattyAcids.J.WaterPollut.ControlFed.,1961,24(11)11691184[3]SiberS,EckenfelderJrWW,EffluentQualityVariationfromMulticomponentSubstratesintheActivatedSludgeProcess.WaterRes.,1980,14(5)471476[4]NamkungE,RittmannBE,SolubleMicrobialProducts(SMP)FormationKineticsbyBiofilms.WaterRes.,1986,20(6)795806[5]SchienerP,NachaiyasitS,StuckeyDC,ProductionofSolubleMicrobialProducts(SMP)inanAnaerobicBaffledReactor:Composition,BiodegradabilityandtheEffectofProcessParameters.Environ.Technol.,1998,19(4)391400[6]GaudyJrAF,BlachlyTR,AStudyoftheBiodegradabilityofResidualCOD.J.WaterPollut.ControlFed.,1985,57(4)332338[7]AquinoSF,StuckeyDC,SolubleMicrobialProductsFormationinAnaerobicChemostatsinthePresenceofToxicCompounds.WaterRes.,2004,38(2)255266[8]DuboisM,ColorimetricMethodforDeterminationofSugarsandRelatedSubstances.Anal.Chem.,1956,28(3)350356[9]HolmeDJ,PeckH,AnalyticalBiochemistry,3rded.AddisonWesleyLongmanLimited:Harlow,England,1998,pp393395[10]BourtonK,AStudyoftheConditionsandMechanismoftheDiphenylamiceReactionfortheColorimetricEstimationofDeoxyribonucleicAcid.Biochem.J.,1956,62(2)315323[11]SalomonLL,JohnsonJE,EnzymaticMicrodeterminationofGlucoseinBloodandUrine.Anal.Chem.,1959,31(3)435456EFFECTOFCr6+ONSOLUBLEMICROBIALPRODUCTSINEFFLUENTSFROMAEROBICSEQUENCINGBATCHREACTORSHUANGGuangtuanJINGangWUJinghuiLIUYongdi(CollegeofResourcesandEnvironmentalEngineering,EastChinaUniversityofScienceandTechnology,Shangha,i200237,China)ABSTRACTTheeffectsofCr6+onamoun,tmoleculeweight(MW)distribution,andcontentofpolysaccharide,protein,DNAinsolublemicrobialproducts(SMP)wereinvestigatedinaerobicsequencingbatchreactors.ThemicrobialpermissibleconcentrationofCr6+was2mgl-1underthisexperimentalcondition.BelowthemicrobialpermissibleconcentrationofCr6+,theamountofSMPwasanexponentialfunctionofconcentrationofCr6+,inwhichSMP=2494e0593C.TheMWdistributionofSMPmanifestedabimodalpatternwithmorethan50%ofSMPhavingaMWlessthan1kDA.ThecontentofDNAinSMPreducedwiththeincreaseofconcentrationofCr6+.Bythetestofsignificance,itwasfoundthatthecontentofDNAwasinlinearrelationwithconcentrationofCr6+atthe5%significantleve.lKeywords:sequencingbatchreactor,solublemicrobialproduc,tpolysaccharide,protein,DNA.