构筑湿地厌氧氨氧化脱氮研究董泽琴

2520066Vol.25Suppl.JournalofLiaoningTechnicalUniversityJun.2006No:20337010200330411969-E-mail:[e-mail]1008-0562(2006)1-0290-031,211.1100162.100039Fluorescentinsituhybridization,FISHX505ANitrogenremovalbyAnammoxinconstructedwetlandsDONGZe-qin1,2SUNTie-heng11.InstituteofAppliedEcology,ChineseAcademyofSciences,Shenyang110016,China;2.GraduateSchooloftheChineseAcademyofSciences,Beijing100039,ChinaAbstractInordertoimprovenitrogenremovalinconstructedwetlands,theprocessofconventionalnitrificationwasamelioratedtostudythefeasibilityofAnammox.Theunsaturatedlayerandsaturatedlayerreedbedfollowedbyahorizontalflowreedbedandconventionalverticalflowreedbedfollowedbythesamedesignhorizontalflowreedbedwerechoosenascomparison.Withfluorescentinsituhybridization(FISH)technology,Anammoxbacteriawerevalidated.Full-scaleexperimentsresultsshowthatAnammoxcanberealisedinconstructedwetlands,theimprovedprocesshashighernitrogenremovalefficiencythantheconventionalone.Keywordsconstructedwetlandsanammoxautotrophicnitrogenremovalfluorescentinsituhybridization(FISH)Anammox[1-4]AINEvieuPhragmitesaustralisEvieuVF1-2VF1-3VF1-2HF3-2VF1-3HF3-2VF1-21Tab.1designparametersofreedsbeds/m2/%VF1-2()2825cm(2~6mm)55cm(2~6mm)5-15cm(15~25mm)15cm(30~60mm)40VF1-32860cm(2~6mm)5-15cm(15~25mm)15cm(30~60mm)40HF3-27×760cm(2~6mm)4020059200512100d90dEvieu101h24h90d2910.174-0.445m•d-10.307-0.706m•d-1VF1-2pHEhpH24h2Cemagref-Lyon224(mg•L-1)Tab.2inletqualitiesduringtheperiodofsampling/(mg•L-1)CODSSTPPO43-KNNH4+NO3-NO2-TN240~51168~1914.2~9.43.0~6.930.7~78.823.5~63.00.05~0.350.006~0.5331.2~78.9375.4122.96.74.855.241.50.150.0955.5SD80.736.11.61.414.712.80.100.1514.8(1)100dVF1-2-25cm-40cmVF1-3-10cm-20cm-30cmHF3-2-10cm-15cm6-20(2)Fluorescentinsituhybridization,FISHIWA[5]Cemagref-AntonyAOBAnammox[6](1)VF1-2VF1-3VF1-3KNNH4+-N56.21%51.85%VF1-2KNNH4+-N39.72%33.05%VF1-3VF1-225cmVF1-2TN37.1%VF1-3TN21.18%VF1-2VF1-31VF1-3VF1-2100dVF1-2-25cm-50cmAnammox1VF1-2VF1-3/(g•m-3•d-1)Fig.1averageremovalloadofammonium,nitrateandnitriteinVF1-2andVF1-3/(g•m-3•d-1)(2)VF1-2VF1-3FISHVF1-2VF1-3FISH23VF1-2AOBAnammox10µmVF1-3AOBAOB20µm-50µmVF1-3AnammoxVF1-3AnammoxAnammoxAOB(3)VF1-2VF1-2VF1-219.75.914.3VF1-2VF1-2pH7.82~8.2625cmpH6.81~7.11pHAnammoxVF1-29.99-11.88-0.26-1.2514.09-0.66-15-10-505101520VF1-2VF1-3(g•m-3•d-1)NH4-NNO3-NNO2-N2VF1-2Fig.2VF1-23VF1-3Fig.3VF1-3292pH6.94~7.18pHHF3-2AnammoxpHVF1-2Eh–148.34~120.18mVEhAnammox(1)HF3-2VF1-2VF1-3VF1-2VF1-3HF3-2VF1-2HF3-256.54%VF1-3HF3-23.67%HF3-2VF1-253.94%VF1-345.52%4HF3-2VF1-3VF1-2HF3-2VF1-2HF3-24VF1-2HF3-2VF1-3HF3-24VF1-2VF1-3HF3-2/(g•m-3•d-1)Fig.4Averageremovalloadofammonium,nitrateandnitriteinHF3-2followedbyVF1-2/VF1-3/(g•m-3•d-1)(2)HF3-2FISHHF3-2AnammoxFISH55HF3-2AnammoxFig.5HF3-2(Anammoxbacteria)HF3-2AOBAnammoxVF1-2AnammoxHF3-2Anammox5µmHF3-2Anammox(3)HF3-2HF3-2FISH-10cm-15cmFISHAnammoxAnammoxVF1-2HF3-2AnammoxpH6.94~7.18VF1-2HF3-2HF3-2VF1-2CNO3-N516.67VF1-3CNO3-N8.43HF3-2VF1-2HF3-2FISHpH6.81~7.18Eh–148.34mV~120.18mV[1]Mulder,A.(2003).Thequestforsustainablenitrogenremovaltechnologies.WaterScience&Technology,48(1):67-75.[2]vandeGraaf,A.A.,Mulder,A.,DeBruijn,P.,Jetten,M.S.M.,Robertson,L.A.&Kuenen,J.G.(1995).Anaerobicoxidationofammoniaisabiologicallymediatedprocess.Applied&EnvironmentalMicrobiology,61:1246-1251.[3]Strous,M.(2000).MicrobiologyandApplicationofAnaerobicAmmoniumOxidation.PhDthesis,TUDelft,144pp.[4]Sliekers,O.,Derwort,N.,Gomez,J.L.C.,Strous,M.,Kuenen,J.G.,andJetten,M.S.M.(2002).Completelyautotrophicnitrogenremovalovernitriteinonesinglereactor.WwaterResearch,36:2475-2482.[5]Amann,R.I.(1995).InsituidentificationofmicroorganismsbywholecellhybridizationwithrRNA-targetednucleicacidprobes.InMolecularMicrobialEcologyManual,pp.MMEM-3.3.6/1-MMEM-3.3.6/15.EditedbyA.D.L.Akkermans,J.D.vanElsas&F.J.deBruijn.London:Kluwer.[6]Alm,E.W.,D.B.Oerther,N.Larsen,D.A.Stahl,andL.Raskin.(1996).Theoligonucleotideprobedatabase.Appl.Environ.Microbiol.62:3557-3559.2.760.091.630.1780.019-0.08-0.500.511.522.53HF3-2(+VF1-2)HF3-2(+VF1-3)(g•m-3•d-1)NO2-NNO3-NNH4-N