固定化光合细菌对水体富营养化的去除效果常会庆

26320105WATERRESOURCESPROTECTIONVol.26No.3May2010　　:([2002]168):(1974—),,,,。E-mail:[e-mail],,(,　471003):为了探索对富营养化水体的微生物修复方法,采用固定化光合细菌进行人工模拟的富营养化水体处理试验。试验结果表明,接种固定化光合细菌比对照可明显地降低水体中的养分。19d的处理时间,TN、NH4-N、NO3-N、TP和COD的去除率分别达到了65.94%,79.84%,78.80%,62.95%,78.06%,并且接种光合细菌对水体中的藻类也起到一定的抑制作用。接种光合细菌增加了水体的DO和pH值,同时也提高了硝化率以及光合细菌数,而水体中这些因素的变化都与水体中养分的去除效果密切相关。因此富营养化水体修复中接种固定化光合细菌可以起到去除养分的目的。:富营养化;固定化光合细菌;养分去除:X171　　　:A　　　:1004-6933(2010)03-0064-04EffectsofimmobilizedphotosyntheticbacteriaoneutrophicwaterCHANGHui-qing,WANGShi-huang,KOUTai-ji(CollegeofAgriculture,HenanUniversityScienceandTechnology,Luoyang471003,China)Abstract:Inordertofindafeasiblebiotechnologytoremediateeutrophicwater,anexperimentoftreatingtheartificialeutrophicwaterwithimmobilizedphotosyntheticbacteriawascarriedout.Theresultsshowedthattheinoculationofimmobilizedphotosyntheticbacteriacouldreducethenutrientsinwatereffectively.TheremovalratesofTN,NH4-N,NO3-N,TP,andCODwere65.94%,79.84%,78.80%,62.95%,and78.06%,respectively,after19daysoftreatment.Furthermore,immobilizedphotosyntheticbacteriainhibitedalgaegrowthinthewater.TheinoculationofimmobilizedphotosyntheticbacteriaalsoincreasedDO,pHvalue,therateofnitrification,andthenumberofphotosyntheticbacteria,andthechangeofallthesefactorshadanimpactonnutrientremovalefficiencyinwater.Therefore,theinoculationofimmobilizedphotosyntheticbacteriacouldachievethegoalofnutrientremovalintheeutrophicwaterremediation.Keywords:eutrophication;immobilizedphotosyntheticbacteria;nutrientremoval　　,[1],,,。2048,61%TN、TPEPA[2]。N、P(GB18918—2002)15mL/L1.5mL/L,30。,,[3-4]。,、,。。,,。,·64·,[5-6],,[7-8]。,,[9-10]。。,,。1　1.1　,120(1.2352),。WFZUV-2100、FA1004A、250D、BCD-185。1.2　1.2.1　试验用品配制a.,:1.0g,0.5g,1.0g,0.1g,1.0g,1.0g,1g,0.5g,3.0g,0.5g,1L。b.(1mL/L),:FeCl2.4H2O1.8g,CoCl2.6H2O0.25g,NiCl6H2O0.01g,CuCl2.H2O0.01g,MnCL24H2O0.7g,ZnCL20.1g,0.5g,Na2SeO3.5H2O0.01g,NaMoO42H2O0.03g,1L。c.(1mL/L),:Biotin0.1g,0.35g,0.3g,0.2g,0.1g,B120.05g,0.1g,1L。pH6.80,30℃3d,(4℃,4000r/min20min),2。1.2.2　细胞固定:50mL4%5min(73.5kPa)45℃,(OD600=0.5)50mL,1mm,5%CaCl2,24h,,,。1.3　2,:①CK-;②P-。3,60L(0.6m,0.6m,0.28m2)。,、NH4NO3NaH2PO4,TN、TP、NH4-N、CODa40.2mg/L、7.99mg/L、19.85mg/L、41.26mg/L75.46μM/L,pH7.02。50g0.5mm,0.3m,3。22.4～35.3℃,23.20～32.6℃,19d。1.4　0d、2d、6d、12d、19d,8:00,100mL0.35m3。[11]。:NO-xNH+4[12]。,2。24hNO-x。NO3-N[13]。24hNO-x。NH3-NNO3-N,NO3-N。2　2.1　、,,TN。TN(13.06±0.85)mg/L18.81mg/L。NH4-N,2NH4-N(10.96±1.04)mg/L(4.01±0.76)mg/L,NH4-N35.02%。NH4-N,NH3-N。NH4-N,NH3-NNH4-N。TN,19d2(0.31±0.22)mg/L(4.23±0.059)mg/L。NO3-N,·65·DO,,NO3-N,98.45%。(1),,、、、、pH[14-15]。,NH3-N,,TN。1CK-P-TN、NH3-N、NO3-N。1　CK-P-0d,10d,19dmg/(L·h)/dCK-P-CK-P-00.832±0.150.104±0.0040.615±0.040.515±0.087100.209±0.010.365±0.0270.577±0.010.248±0.038191.150±0.531.546±0.1340.990±0.060.189±0.0251　CK-P-TN、NH3-N、NO3-N2.2　TP22TP。22pHTP,TP。CK-P-TP(3.94±0.17)mg/L(2.96±0.15)mg/L。TP,2　CK-P-TP。pHP[16],PpHCa2+。,[17-18]。2.3　CODaCOD(3)。COD78.06%,COD59.39%。COD,(2),,C/N,,[19]。a,a。。3　CK-P-CODa2　CK-P-/dlgc/(·mL-1)CK-P-01.14±0.064.70±0.01101.78±0.034.84±0.01190.55±0.134.00±0.01　　:c。·66·2.4　pHDOpH7.02,pH。,pH2(4)。pH。DO2.64mg/L,DO,。6,DO。2DO。4　CK-P-pHDO3　　　,。:19dTN、NH4-N、、TPCOD65.94%、79.84%、78.80%、62.95%、78.06%。,。。:[1]CARPENTARSR,CARACONF,CORRELLDF,etal.Nonpointpollutionofsurfacewaterswithnitrogenandphosphorus[J].EcologicalApplications,1998,8(3):559-568.[2]SMITHVH,SCHWARTZGE,ALEXANDERRB.Regionalinterpretationofwaterqualitymonitoringdata[J].WaterResourceResaerch,1997,33(12):2781-2798.[3],,,.[J].,2008,3(1):56-59.[4].[J].,2008(2):16-18;22.[5].[J].,2008,24(6):93-95.[6],,.[J].,1998,18(5):36-38.[7],.[J].,2008,28(2):86-88.[8],.[J].,2008,6(4):55-58.[9],,,.[J].,2006,26(6):4-7.[10]NAGADOMIH,KITAMURAT,WATANABEM,etal.Simultaneousremovalofchemicaloxygendemand(COD),phosphate,nitrateandH2Sinthesyntheticsewagewastewaterusingporousceramicimmobilizedphotosyntheticbacteria[J].BiotechnologyLetters,2000,22:1369-1374.[11].[M].4.:,2002:200-284.[12]BELSERW,MAYSEL.Useofnitrifieractivitymeasurementstoestimatetheefficiencyofviablenitrifiercountsinsoilandsediments[J].ApplEnvironMicrobiol,1982,43:945-948.[13]ANDERSENJM.Ratesofdenitrificationofundisturbedsedimentfromsixlakesasafunctionofnitrateconcentration,oxygenandtemperature[J].ArchHydrobiol,1977,80:147-159.[14]KEMPMC.Assessmentandenhancementofnitrogentransformationandremovalinasubsurfaceflowconstructedwetlandssystemtreatingmunicipalwastewater[D].Tennessee:TennesseeTechnologicalUniversity,1995.[15]BEROUNSKYV,NIXONS.TemperatureandtheannualcycleofnitrificationinwatersofNarragansettBay[J].LimnolOceanogr,1990,35:1610-1617.[16]REDDYKR.Fateofnitrogenandphosphorusinawastewaterretentionreservoircontainingaquaticmacrophytes[J].JEnvironQual,1983,12:137-141.[17]GUMBRICHTT.Nutrientremovalprocessesinfreshwatersubmersedmacrophytesystems[J].EcolEng,1993,2:1-30.[18]GUMBRICHTT.Nutrientremovalcapacityinsubmersedmacrophytepondsystemsinatemperateclimate[J].EcolEng,1993,2:49-61.[19],,,.SBRDOC/N[J].:,2003,21(2):7-10.(:2008-12-05　:)·67·