固定化解脂耶氏酵母处理油脂废水的性能研究

24环境工程学报Vol.2,No.420084ChineseJournalofEnvironmentalEngineeringApr.2008(Yarrowialipolytica)吴兰1,2万金保2*(1.,330047;2.,,330047),,)))(Yarrowialipolyt-ica),,,:25~35epH4~8100~200r/min,2000mg/L,6165@106/mL,4mL/150L,pHYPD30h,:82%,20%,X703A1673-9108(2008)04-0482-05InvestigationoncapabilityofdisposinggreasewastewaterwithimmobilizedYarrowialipolyticaWuLan1,2WanJinbao2(1.CollegeofLifeScience,NanchangUniversity,Nanchang330047;2.KeyLaboratoryofPoyangLakeEcologyandBio-resourceUtilization,MinistryofEducation,CollegeofEnvironmentalScienceandEngineering,NanchangUniversity,Nanchang330047)AbstractTheoptimalconditionshavebeenstudiedindisposinggreasewastewaterwithpredominantdispo-singoilbacteria)))immobilizedYarrowialipolyticaembeddedintomicroorganismswhenusingsodiumalginateasimmobilizedcarrierandcalciumchlorideascrosslinker.Theresultsshowthattheoptimalconditionsofdispo-singsaladoilforimmobilizedYarrowialipolyticaare:temperature25~30e,pH4~8,theshakingspeed100~200r/min,primaryoilconcentration2000mg/L,embeddedbacteriaconcentration6165@106entries/mL,embeddingquantity4mL/150L.Incontrastwithsuspendedbacteria,theembeddedmicroorganismsrepresentmoreadaptablecapabilityofalargerrangeoftemperatureandanenhancementofheatresistance,apHavertiontoacidity.TheresultofplungingtheimmobilizedYarrowialipolyticaintoYPDliquidsubstratetodomesticatefor30hbeforedisposingsaladoilshowstha:tafterdomestication,disposingrateofsaladoilfortheimmobilizedm-icroorganismscanbe82%,withanincreaseof20%comparedwiththeonesthathavent'beendomesticatedwiththesamecondition,furthermore,themechanicalstrength,thestabilityandtherepetitionforutilizationarepro-motedobviously.Keywordsimmobilization;sodiumalginate;Yarrowialipolytica;reasewastewater:(2007-41):2007-08-24;:2007-09-30:(1969~),,,,E-mai:[e-mail]*,,,,,,4:(Yarrowialipolytica),[1](Yarrowialipolytica),[2,3],,,11.11.1.1实验菌种W291.1.2化学试剂(30~60e),1.1.3培养基YPD,:1%2%2%pH1.1.4废水来源,()1.21.2.1解脂耶氏酵母的活化与培养YPD,30e160r/min18h,,4e1.2.2固定化微生物的制备:4%,4%,4e24h[4]1.2.3油含量的测定(CJ/T57-1999)1.2.4降解率的计算=(-)/@100%1.2.5接种菌浓度记数22.1[2],2000mg/L,600nmOD[5],5h,11Fig.1GrowthofYarrowialipolytica:,405h,OD010225;5h20h,OD01022511119;20h35h;35h,OD,,35h50h,OD1113701996,,,2.24mL2000mg/L5000mg/L150mL50h,pH150r/min,,2:30e,,[6]483225~30e,25~35e,30e,,,30e40e,38%,7%,2Fig.2EffectoftemperatureondegradationofimmobilizedYarrowialipolytica2.3pH2000mg/L5000mg/L150mL50h,30e,150r/min,pH,3:pH6~8pH,pH,pH,,pH4,13%2pH6~10,60%,;,pH4~8,,,3pHFig.3EffectofpHondegradationofimmobilizedYarrowialipolyticapH8,,pH=10,1317%,2.4,,,,2000mg/L5000mg/L150mL50h,30e,pH6,,44Fig.4EffectofrotaryvelocityondegradationofimmobilizedYarrowialipolytica4:,,4844:(Yarrowialipolytica)50r/min200r/min,27147%76145%;44167%52138%,,,,,,,150r/min2.5,1000mg/L2000mg/L3000mg/L4000mg/L5000mg/L50h,55Fig.5EffectofoilconcentrationondegradationofimmobilizedYarrowialipolytica:5,,,,1000mg/L,70%,5000mg/L70%2000mg/L3000mg/L,66%32%,30%5000mg/L,,5:2000mg/L,,2000mg/L2.6,6165@106/mL24681012mL,2000mg/L150mL50h,6:,[4mL,,,,4mL12mL,8%[2]4mL/150mL(215%),6Fig.6EffectofquantitycellsondegradationofimmobilizedYarrowialipolytica2.7,YPD,30e160r/min485230h,2000mg/L,77Fig.7Effectofacclimationondegradation:,,20h,10%,20%,,:,,50h82%,20%,10%,,,,,,1270%,12,,(),3(1),,:,(2):25~35epH4~8100~200r/min,2000mg/L,6165@106/mL,4mL/150L,pH(3)YPD[1],,,..,2004,27(3):102~104[2],,,..(),2006,30(4):379~382[3],,,..,2006,19(5):122~125[4],..,2000,21(6):80~84[5],..:,2002.75~77[6],,,.UP-2.,2006,26(supp.l):92~96486