固定化耐冷菌用于内循环复合生物反应器处理生活污水及其微生物学研究

固定化耐冷菌用于内循环复合生物反应器处理生活污水及其微生物学研究固定化耐冷菌用于内循环复合生物反应器处理生活污水及其微生物学研究贲岳,陈忠林,徐贞贞and姜安玺Citation:科学通报53,2661(2008);doi:10.1360/csb2008-53-21-2661Viewonline::《中国科学》杂志社Articlesyoumaybeinterestedin膜生物反应器去除污水中病毒的试验研究ChineseScienceBulletin50,117(2005);含萘酰亚胺基团的亲水性荧光聚合物作为微生物反应器的荧光探针的研究ScienceinChinaSeriesB-Chemistry(inChinese)39,663(2009);膜生物反应器处理垃圾渗滤液的效能及有机污染物的分子量分布ChineseScienceBulletin51,1757(2006);构造微生物学:地球生物学研究的新理念SCIENTIASINICATerrae48,956(2018);固定化微生物处理垃圾渗滤液ScienceinChinaSeriesB-Chemistry(inChinese)38,721(2008);2008年第53卷第21期:2661~2667SCIENCEINCHINAPRESS,*,,,,150090*,E-mail:[e-mail]2008-07-02,2008-09-17(:E01-22)(:2003AA4CS122),6,.COD63.67%,,(ICCBR).,12,ICCBR,,,.:ICCBR4~10COD85.79%,COD86.66%,COD60mg·L−1,B.,110000±30000·mL−135000±20000·mL−1,.(),,,[1,2].,.,.,.,0~40,20,,20,15.,.,,(10).[3~6][7],[8][9],[10][11,12].,[13,14],.,10,,.,,.,,[15,16][17][18].:2019-07-2109:10:17年11月第53卷第21期,,.,,,,,,,.11.141,LB,4,.[19]..LB,4.,25,.,(4.0~6.0mm,0.06kg·L−1,90%~98%),30%.1.2(ICCBR)1.0.5t·d−1,,112.75L,,.,,,,,.,,,.ICCBR1,4~28.,4~624~26.1.3,,1.1.4(COD).,4℃.COD5B-1COD(,);OlympusBX51();HitachiS-52011,;2,;3,;4,;5,;6,;7,;8,;9,;10,;11,;12,;13,;14,2662DownloadedtoIP:192.168.0.24On:2019-07-2109:10:17~586mg·L−150~300mg·L−12.1~6.7mg·L−17.5~8.5().(DO)leve2().400.:50mL,,.22.1().6,H1,H2,H3,H4,H5,H6.2.().,RNA[4].,,,,.,,,,COD,2.2:,COD63.67%;,,2CODCOD9.55,COD,,COD.,COD6.02%,,.2.2,,.().2H1a)H2H3H4H5H6/μm0.23×1.030.43×0.860.4×0.960.67×0.710.31×2.540.43×1.86−−−++−+++++++++−++++−+−+V.P+−−−−−M.R+−−+−−−−−−−++−−−−+++−−−+a)H12663DownloadedtoIP:192.168.0.24On:2019-07-2109:10:17年11月第53卷第21期,.ICCBR,(4~1020~28,4~10),.3,COD145~586mg·L−1,COD21~59mg·L−1,49.40mg·L−1,86.66%.3ICCBR1COD,.3,ICCBR,.COD85.79%,87.53%.4,,.,ICCBR,B(GB18918-2002).4(a)(b)(×4000)().,.,,DO.4,5,COD,.,.5()DO.ICCBR,.,[20].,DO,(,,),(4),COD300mg·L−1,CODDO6.DO2.0~4.0mg·L−14.0~8.0mg·L−1,COD82%,.()COD.,.7CODCOD,,COD,,.2664DownloadedtoIP:192.168.0.24On:2019-07-2109:10:17(4),COD,COD,,7,COD800mg·L−1COD1000mg·L−1,,COD100mg·L−1.2.3.8,,,,,,.Wilen[21]204,,.,,9,10,11.,.,,8(a)(b)(×10)9(a)(b)(×3000)(c)(×4000)10(×40)2665DownloadedtoIP:192.168.0.24On:2019-07-2109:10:17年11月第53卷第21期11(×40),,,;,().110000±30000·mL−135000±20000·mL−1.,3..21421mg81972mg,121347mg.,,4,(2),(1),(1),(2).ICCBR1,,,(3).,,.3(mg·g−1)2581214211755197213473(1)6,,COD63.67%.(2),DO.ICCBR,COD86.66%.(3).,,B.1CavicchioliR,ThomasT.Encylopediaofmicrobiology,2nded.In:LederbergJ,AlexanderM,BloomBR,etal,eds.SanDiego:Aca-demicPress,2000.317—3372RussellNJ,HamamotoT.Extremophiles:Microbiallifeinextremeenvironments.In:HorikoshiK,GrantWD,eds.NewYork:Wiley-Liss,1998.25—453RusselNJ.Molecularadaptationsinpsychrophilicbacteria:Potentialforbiotechnologicalapplications.AdvBiochemEngBiotechnol,1998,61:1—214朱非,王珊,周培瑾.低温酶冷适应的分子机制及其在生物技术中的应用.微生物学报,2002,42(5):640—6445GianeseG,ArgosP,PascarellaS.Structuraladaptationofenzymestolowtemperatures.ProteinEng,2001,14(3):141—148[doi]6RussellNJ.Towardsamolecularunderstandingofcoldactivityofenzymesfrompsychrophiles.Extremophiles,2000,4(2):83—90[doi]7PapaleoE,RiccardiL,VillaC,etal.Flexibilityandenzymaticcold-adaptation:Acomparativemoleculardynamicsinvestigationoftheelastasefamily.BiochimicaetbiophysicaActa,2006,1764:1397—14068IrwinJA,AlfredssonGA,LanzettiAJ,etal.PurificationandcharacterisationofaserinepeptidasefromthemarinepsychrophilestrainPA-43.FEMSMicrobiolLett,2001,201:285—290[doi]9RicardoC,KhawarSS,DavidA,etal.Low-temperatureextremophilesandtheirapplications.CurrOpinBiotechnol,2002,13(3):253—2666DownloadedtoIP:192.168.0.24On:2019-07-2109:10:17(4):257—264[doi]11MargesinR,SchinnerF.Efficiencyofindigenousandinoculatedcold-adaptedsoilmicroorganismsforbiodegradationofdieseloilinalpinesoils.ApplEnvironMicrobiol,1997,63(7):2660—266412MargesinR,SchinnerF.Low-temperaturebioremediationofawastewatercontaminatedwithanionicsurfactantandfueloil.ApplMicrobiolBiotechnol,1998,49(4):482—486[doi]13UedaM,AsanoT,NakazawaM,etal.Purificationandcharacterizationofnovelraw-starch-digestingandcold-adaptedα-amylasesfromEiseniafoetida.CompBiochemPhysiol,PartB,2008,150:125—130[doi]14YangYR,ZhuH,FangN,etal.Cold-adaptedmaturationofthermophilicWF146proteasebymimickingthepropeptidebindingin-teractionsofpsychrophilicsubtilisinS41.FEBSLetters,2008,582:2620—2626[doi]15ChevalierP.Nitrogenandphosphorasremovalbyhighlatitudematfo